FastQCFastQC Report
Wed 25 May 2016
SRR2088448_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088448_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences689348
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT73661.0685459303573812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61010.8850391964581024No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT52850.7666664732471843No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26600.3858718673297087No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG16760.24312828934007205No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA15610.2264458589855922No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14260.20686213639555057No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13680.1984483889124216No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA12590.18263634622861022No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG11320.16421314053279332No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8840.128237116811828No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7990.11590662481069068No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7210.10459158509199998No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA6970.10111003440932591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGAGC400.001930406823.12500237
GCGCTAT450.003824278720.5555556
TGCACCG656.89701E-519.9230775
GGTATCA20400.019.8602941
GTAAGAT1052.2555469E-819.380953
CTAATAC2250.018.9111123
CATATAG609.2317007E-418.53
ATTAGAC609.2317007E-418.53
CGAGTAA609.2317007E-418.510
CGTAGTA902.1496744E-618.52
AATACTG2600.018.55
CTTACTC609.2317007E-418.53
TTGCGAT500.00703209618.49999811
GCGATAA500.00703209618.49999813
ACCGTTA1002.8707836E-718.4999988
AGTAACG500.00703209618.49999812
TAATCGG500.00703209618.49999816
TAATACT2600.017.7884624
CTACACT953.6021502E-617.5263164
TAAGATT1603.45608E-1117.343754
Produced by FastQC (version 0.11.5)