##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088448_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	689348
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.38434288632157	31.0	31.0	34.0	30.0	34.0
2	31.610900445058228	31.0	31.0	34.0	30.0	34.0
3	31.726155729762034	31.0	31.0	34.0	30.0	34.0
4	35.427685291028624	37.0	35.0	37.0	33.0	37.0
5	35.29517166946158	37.0	35.0	37.0	33.0	37.0
6	35.34676534928657	37.0	35.0	37.0	33.0	37.0
7	35.19578355199406	37.0	35.0	37.0	32.0	37.0
8	35.227632487509936	37.0	35.0	37.0	32.0	37.0
9	36.77830355640402	39.0	37.0	39.0	32.0	39.0
10	36.6104986160836	39.0	35.0	39.0	32.0	39.0
11	36.64915543383023	39.0	35.0	39.0	32.0	39.0
12	36.507338818709854	38.0	35.0	39.0	32.0	39.0
13	36.5227780453414	38.0	35.0	39.0	32.0	39.0
14	37.49260170479932	39.0	36.0	41.0	32.0	41.0
15	37.50434468512275	39.0	36.0	41.0	32.0	41.0
16	37.37490063074093	39.0	36.0	41.0	32.0	41.0
17	37.439779327712564	39.0	36.0	41.0	32.0	41.0
18	37.31345996506844	39.0	36.0	41.0	32.0	41.0
19	37.397181104463925	39.0	36.0	41.0	32.0	41.0
20	37.31378781109106	39.0	36.0	41.0	31.0	41.0
21	37.141664297278005	39.0	36.0	40.0	31.0	41.0
22	37.05351288463882	39.0	36.0	40.0	31.0	41.0
23	36.868552603329526	39.0	36.0	40.0	30.0	41.0
24	37.0626737148726	39.0	36.0	40.0	31.0	41.0
25	37.02226886855406	39.0	36.0	41.0	31.0	41.0
26	36.85853299059401	39.0	36.0	40.0	30.0	41.0
27	36.79202521803211	39.0	35.0	40.0	30.0	41.0
28	36.614667773026106	39.0	35.0	40.0	30.0	41.0
29	36.45048944800014	39.0	35.0	40.0	30.0	41.0
30	36.26846092249488	38.0	35.0	40.0	30.0	41.0
31	36.00566042115158	38.0	35.0	40.0	29.0	41.0
32	35.876526805038964	38.0	35.0	40.0	29.0	41.0
33	35.66192257031282	38.0	34.0	40.0	27.0	41.0
34	35.54845883356447	38.0	34.0	40.0	27.0	41.0
35	35.303436000394576	38.0	34.0	40.0	26.0	41.0
36	35.16239257965498	38.0	34.0	40.0	25.0	41.0
37	35.00085152927114	38.0	33.0	40.0	25.0	41.0
38	34.94209310827043	38.0	33.0	40.0	25.0	41.0
39	34.84616913373216	38.0	33.0	40.0	24.0	41.0
40	34.73528754707347	38.0	33.0	40.0	24.0	41.0
41	34.53858718673297	38.0	33.0	40.0	23.0	41.0
42	34.320903230298775	38.0	33.0	40.0	22.0	41.0
43	33.99505764867672	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	1.0
14	1.0
15	3.0
16	9.0
17	24.0
18	62.0
19	138.0
20	295.0
21	562.0
22	1128.0
23	1908.0
24	3023.0
25	4609.0
26	6822.0
27	9325.0
28	12820.0
29	16421.0
30	20751.0
31	25843.0
32	31552.0
33	38704.0
34	46906.0
35	57321.0
36	72072.0
37	95162.0
38	122417.0
39	121468.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.61439505155597	21.292873846011013	13.374086818268857	23.718644284164167
2	19.070048799735403	23.41531418093619	34.785913645937896	22.72872337339051
3	19.616217063079898	25.117647400152027	32.25758252725764	23.008553009510436
4	13.70120751782844	16.452793074035174	36.824796764478904	33.02120264365748
5	12.496590981623216	38.65812332813035	35.348474210413315	13.496811479833118
6	32.21174210993576	37.92757794321591	13.832490991487608	16.02818895536072
7	26.3219738071337	32.70365620847526	21.857610379663104	19.116759604727946
8	27.093282347957782	34.35652239507477	19.132861776635313	19.41733348033214
9	24.875969756929734	14.367779408948747	21.42720367651752	39.329047157603995
10	16.476148476531446	27.395306869679754	33.74318921647702	22.38535543731178
11	33.544740827564596	22.61049571479137	23.282725125771016	20.562038331873016
12	21.14360236049136	27.279980503316175	30.67956968033562	20.896847455856836
13	30.937059366241726	21.086591968062578	26.0895802990652	21.886768366630495
14	20.7680300805979	22.698985127976	27.506571426913546	29.026413364512553
15	23.016531563158228	29.5576399728439	25.19598229051219	22.229846173485672
16	22.293964731891585	28.2741082878314	26.150942629847336	23.28098435042968
17	21.947985632800847	26.93574217956678	26.750204541102608	24.366067646529764
18	22.80444710073867	25.88010699965765	29.10677915943761	22.20866674016607
19	22.873787985168594	25.137521251965627	29.879683410991255	22.109007351874524
20	23.654235596534697	25.023935660943387	29.815419787973564	21.506408954548355
21	22.78022131057173	25.67846718928611	30.095684617928825	21.445626882213336
22	21.74097843179352	26.272506774517367	29.628721632615168	22.357793161073943
23	22.622971271404282	26.213030283688354	30.404527176404372	20.759471268502992
24	22.534481858219653	26.40524089429432	28.204622338789697	22.855654908696334
25	22.347638638249474	26.4570289606991	29.446665544833667	21.74866685621776
26	22.254362092876168	27.02104017129229	29.338592409058993	21.386005326772544
27	21.475945386074958	27.072393043861737	28.778643007595583	22.67301856246772
28	21.668010932069144	26.8250578807801	29.221090073518745	22.285841113632014
29	22.238695114804134	26.767177100680644	28.65388744146643	22.340240343048794
30	21.972356487579567	26.822301653156316	29.94960455386829	21.255737305395822
31	22.496619994545572	26.76064919315063	29.698642775492207	21.044088036811594
32	21.962927287813994	26.482850461595596	28.95779780314152	22.596424447448893
33	21.645670981855318	26.574676360851125	29.644968869134313	22.134683788159247
34	21.378026773124752	26.90005628506937	30.020976342863108	21.70094059894277
35	20.93528957797803	28.784590656678482	28.90644493057208	21.373674834771407
36	22.87712447123949	26.86248455061885	28.78821727197294	21.472173706168725
37	21.820328774436135	27.346565160122317	28.76210564185288	22.071000423588664
38	21.687594654659183	26.121349449044608	29.985145383753924	22.20591051254229
39	21.24094071499446	26.103796631019456	30.651427145650672	22.003835508335413
40	20.35329035552435	26.829699948357	30.254965561661162	22.562044134457487
41	21.57052751295427	25.377022926011243	30.417002733017284	22.6354468280172
42	20.342990768088107	27.27229207889194	29.951200264597848	22.433516888422105
43	20.895396809739058	26.620516778173	29.332064501528983	23.15202191055896
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	73.0
2	106.0
3	279.5
4	453.0
5	453.0
6	720.0
7	987.0
8	1089.5
9	1192.0
10	1808.0
11	2424.0
12	2424.0
13	4609.0
14	6794.0
15	10838.5
16	14883.0
17	13720.5
18	12558.0
19	12558.0
20	13396.0
21	14234.0
22	11498.0
23	8762.0
24	9997.0
25	11232.0
26	11232.0
27	12617.5
28	14003.0
29	15710.5
30	17418.0
31	19381.0
32	21344.0
33	21344.0
34	24016.5
35	26689.0
36	29563.5
37	32438.0
38	34881.0
39	37324.0
40	37324.0
41	38445.5
42	39567.0
43	42042.5
44	44518.0
45	45567.0
46	46616.0
47	46616.0
48	56856.0
49	67096.0
50	64117.0
51	61138.0
52	56658.0
53	52178.0
54	52178.0
55	47027.5
56	41877.0
57	36009.5
58	30142.0
59	26733.0
60	23324.0
61	23324.0
62	20719.5
63	18115.0
64	15663.0
65	13211.0
66	11387.5
67	9564.0
68	9564.0
69	8232.5
70	6901.0
71	5727.5
72	4554.0
73	3875.0
74	3196.0
75	3196.0
76	2594.5
77	1993.0
78	1570.5
79	1148.0
80	891.0
81	634.0
82	634.0
83	483.5
84	333.0
85	256.5
86	180.0
87	135.5
88	91.0
89	91.0
90	74.5
91	58.0
92	38.5
93	19.0
94	14.5
95	10.0
96	10.0
97	6.0
98	2.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	689348.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.25247261576359
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.14183927163567	52.505112844015
2	7.355850912829371	8.864164113817825
3	1.9511910421642151	3.526922545083677
4	0.8997555658449454	2.1684999036781374
5	0.5155580068477913	1.5531822344717097
6	0.35385499015318383	1.2792382862493603
7	0.275851593869634	1.16345184239514
8	0.2097275429179911	1.0109282429150113
9	0.15266256363445233	0.8278467241353396
>10	0.989940296212473	11.237992944489752
>50	0.08993996100949586	3.720534780452796
>100	0.058025684437138826	6.488202053376855
>500	0.003384831592146445	1.307866769236465
>1k	0.0016924157960732226	1.6143661769523845
>5k	7.253210554599525E-4	2.7316905387304735
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	7366	1.0685459303573812	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6101	0.8850391964581024	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5285	0.7666664732471843	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2660	0.3858718673297087	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1676	0.24312828934007205	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1561	0.2264458589855922	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1426	0.20686213639555057	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1368	0.1984483889124216	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1259	0.18263634622861022	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1132	0.16421314053279332	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	884	0.128237116811828	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	799	0.11590662481069068	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	721	0.10459158509199998	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	697	0.10111003440932591	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4506461177808596E-4	0.0
2	0.0	0.0	0.0	1.4506461177808596E-4	0.0
3	0.0	0.0	0.0	1.4506461177808596E-4	0.0
4	0.0	0.0	0.0	2.901292235561719E-4	0.0
5	0.0	0.0	0.0	4.351938353342579E-4	0.0
6	0.0	0.0	0.0	4.351938353342579E-4	0.0
7	0.0	0.0	0.0	4.351938353342579E-4	0.0
8	0.0	0.0	0.0	4.351938353342579E-4	0.0
9	2.901292235561719E-4	0.0	0.0	5.802584471123438E-4	0.0
10	4.351938353342579E-4	0.0	0.0	5.802584471123438E-4	0.0
11	4.351938353342579E-4	0.0	0.0	8.703876706685158E-4	0.0
12	4.351938353342579E-4	0.0	0.0	0.0010154522824466017	0.0
13	4.351938353342579E-4	0.0	0.0	0.0011605168942246877	0.0
14	4.351938353342579E-4	0.0	0.0	0.0014506461177808596	0.0
15	4.351938353342579E-4	0.0	0.0	0.0014506461177808596	0.0
16	4.351938353342579E-4	0.0	0.0	0.0018858399531151175	1.4506461177808596E-4
17	4.351938353342579E-4	0.0	0.0	0.0026111630120055473	1.4506461177808596E-4
18	4.351938353342579E-4	0.0	0.0	0.003046356847339805	1.4506461177808596E-4
19	4.351938353342579E-4	0.0	0.0	0.004061809129786407	1.4506461177808596E-4
20	4.351938353342579E-4	0.0	0.0	0.006382842918235782	1.4506461177808596E-4
21	4.351938353342579E-4	0.0	0.0	0.011024910495134533	1.4506461177808596E-4
22	4.351938353342579E-4	0.0	0.0	0.01682749496625797	1.4506461177808596E-4
23	4.351938353342579E-4	0.0	0.0	0.019583722590041604	1.4506461177808596E-4
24	5.802584471123438E-4	0.0	0.0	0.02553137167294313	1.4506461177808596E-4
25	5.802584471123438E-4	0.0	0.0	0.030028374638063793	1.4506461177808596E-4
26	5.802584471123438E-4	0.0	0.0	0.03626615294452149	1.4506461177808596E-4
27	5.802584471123438E-4	0.0	0.0	0.07630398579527321	1.4506461177808596E-4
28	5.802584471123438E-4	0.0	0.0	0.1621822359679001	1.4506461177808596E-4
29	5.802584471123438E-4	0.0	0.0	0.2718510824721331	1.4506461177808596E-4
30	5.802584471123438E-4	0.0	0.0	0.4111131097790956	1.4506461177808596E-4
31	5.802584471123438E-4	0.0	0.0	0.5725700226881053	1.4506461177808596E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGAGC	40	0.0019304068	23.125002	37
GCGCTAT	45	0.0038242787	20.555555	6
TGCACCG	65	6.89701E-5	19.923077	5
GGTATCA	2040	0.0	19.860294	1
GTAAGAT	105	2.2555469E-8	19.38095	3
CTAATAC	225	0.0	18.911112	3
CATATAG	60	9.2317007E-4	18.5	3
ATTAGAC	60	9.2317007E-4	18.5	3
CGAGTAA	60	9.2317007E-4	18.5	10
CGTAGTA	90	2.1496744E-6	18.5	2
AATACTG	260	0.0	18.5	5
CTTACTC	60	9.2317007E-4	18.5	3
TTGCGAT	50	0.007032096	18.499998	11
GCGATAA	50	0.007032096	18.499998	13
ACCGTTA	100	2.8707836E-7	18.499998	8
AGTAACG	50	0.007032096	18.499998	12
TAATCGG	50	0.007032096	18.499998	16
TAATACT	260	0.0	17.788462	4
CTACACT	95	3.6021502E-6	17.526316	4
TAAGATT	160	3.45608E-11	17.34375	4
>>END_MODULE