FastQCFastQC Report
Wed 25 May 2016
SRR2088447_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088447_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2182366
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT239431.0971120334536004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT195670.8965957130930376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT165750.7594968030110439No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84620.3877443105326971No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52340.23983144898701683No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA50520.23149187624807205No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG45940.2105054789160022No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA41480.19006894352276382No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA40240.18438703682150473No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG37610.17233589599544716No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA28650.1312795378960266No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25900.11867853513113748No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA21860.10016651652380948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71550.021.2278141
ACGTTTA1151.3460522E-1020.91304426
CGAATTA909.4880306E-820.55555515
TATGTCG555.14513E-420.1818185
GTATTGG7900.019.9050641
TATACCG953.6093952E-617.5263165
GGACCGT3750.016.7733336
TGTTACG2003.6379788E-1215.72516
TAATACT7250.015.5655174
AGTTCCG1802.0190782E-1015.41666632
TTGGACC12050.015.1991714
TTATGCG3050.015.1639344
TGGACCC11650.015.0858375
ATTGGAC10250.014.8000013
CCGAATT1252.9615112E-614.80000114
GAACCGT750.004105977714.86
CGTTATT2151.2732926E-1114.62790710
CTAATAC6350.014.5669293
CGTGTCT1803.3342076E-914.38888835
TACTGGT6050.014.3719017
Produced by FastQC (version 0.11.5)