##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088447_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2182366
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.325571421109018	31.0	31.0	34.0	30.0	34.0
2	31.550121748597622	31.0	31.0	34.0	30.0	34.0
3	31.659006326161606	31.0	31.0	34.0	30.0	34.0
4	35.37011482033719	37.0	35.0	37.0	33.0	37.0
5	35.231292093076966	37.0	35.0	37.0	33.0	37.0
6	35.28625491782771	37.0	35.0	37.0	32.0	37.0
7	35.12910483392795	37.0	35.0	37.0	32.0	37.0
8	35.1632508021111	37.0	35.0	37.0	32.0	37.0
9	36.70123068266276	39.0	35.0	39.0	32.0	39.0
10	36.5336382623263	38.0	35.0	39.0	32.0	39.0
11	36.57607523211047	38.0	35.0	39.0	32.0	39.0
12	36.42149483633818	38.0	35.0	39.0	32.0	39.0
13	36.44659099344473	38.0	35.0	39.0	32.0	39.0
14	37.38865295738662	39.0	36.0	41.0	32.0	41.0
15	37.40216810562481	39.0	36.0	41.0	32.0	41.0
16	37.26955881827338	39.0	36.0	41.0	32.0	41.0
17	37.342415525168555	39.0	36.0	40.0	32.0	41.0
18	37.21257250158773	39.0	36.0	40.0	31.0	41.0
19	37.30499054695684	39.0	36.0	40.0	31.0	41.0
20	37.21239792042215	39.0	36.0	40.0	31.0	41.0
21	37.04848041071021	39.0	36.0	40.0	31.0	41.0
22	36.96349100013472	39.0	36.0	40.0	31.0	41.0
23	36.776121878731615	39.0	35.0	40.0	30.0	41.0
24	36.96145651096104	39.0	36.0	40.0	31.0	41.0
25	36.932661157660995	39.0	36.0	40.0	31.0	41.0
26	36.753769074481546	39.0	35.0	40.0	30.0	41.0
27	36.687315509864064	39.0	35.0	40.0	30.0	41.0
28	36.49800904156315	39.0	35.0	40.0	30.0	41.0
29	36.32026708627242	38.0	35.0	40.0	30.0	41.0
30	36.144422154670664	38.0	35.0	40.0	29.0	41.0
31	35.88816724600731	38.0	35.0	40.0	29.0	41.0
32	35.74552985154644	38.0	34.0	40.0	28.0	41.0
33	35.53973806410107	38.0	34.0	40.0	27.0	41.0
34	35.4246267582981	38.0	34.0	40.0	27.0	41.0
35	35.191710739628455	38.0	34.0	40.0	26.0	41.0
36	35.05004476792619	38.0	33.0	40.0	25.0	41.0
37	34.88176960234901	38.0	33.0	40.0	25.0	41.0
38	34.806870616569356	38.0	33.0	40.0	24.0	41.0
39	34.71806058195555	38.0	33.0	40.0	24.0	41.0
40	34.59854946420536	38.0	33.0	40.0	24.0	41.0
41	34.39522884795676	38.0	33.0	40.0	23.0	41.0
42	34.18022366550799	38.0	33.0	40.0	22.0	41.0
43	33.84358673109827	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	1.0
13	3.0
14	4.0
15	19.0
16	38.0
17	80.0
18	204.0
19	474.0
20	1071.0
21	2044.0
22	3863.0
23	6578.0
24	10452.0
25	15782.0
26	22699.0
27	31567.0
28	41952.0
29	54728.0
30	68342.0
31	84207.0
32	102573.0
33	124720.0
34	151035.0
35	182568.0
36	227796.0
37	300324.0
38	382908.0
39	366332.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.52630676980855	21.35860804283058	13.422863076129302	23.692222111231573
2	19.106373541376655	23.624039230816464	34.66398395136288	22.605603276444004
3	19.52633059715923	25.16809737688362	32.2707556844269	23.03481634153025
4	13.753238457710577	16.534806718946317	36.66424421934726	33.047710603995846
5	12.563703796705045	38.723018961988956	35.10240720392454	13.610870037381448
6	32.29971507987203	37.92878921317506	13.752734417599981	16.01876128935293
7	26.373119815832908	32.676691260769275	21.725365956031204	19.224822967366613
8	27.15566499844664	34.324764956932064	19.031913070493218	19.48765697412808
9	24.953651220739324	14.302183959977382	21.276724435772916	39.46744038351037
10	16.366732252976814	27.52741749092499	33.67950197171327	22.42634828438493
11	33.539378820967706	22.66365953281897	23.207839564949236	20.589122081264097
12	21.240800122435925	27.36979040179328	30.635282991029	20.75412648474179
13	31.153298759236534	21.02080952507508	25.908211546550852	21.917680169137533
14	20.854842863204432	22.72648125933047	27.489889413599734	28.928786463865364
15	23.033808261309055	29.450788731129425	25.24604030671299	22.26936270084853
16	22.259327720464853	28.226292015179855	26.195880984216213	23.31849928013908
17	21.991865709051552	26.865429538400065	26.86006838449646	24.28263636805192
18	22.932908595533473	25.844794136272288	29.11276110423275	22.1095361639615
19	23.077338998133218	24.99704449207878	29.941861264334214	21.983755245453786
20	23.614370825058675	24.974683439899632	29.85727416941063	21.553671565631063
21	22.693535364828815	25.681347674954612	30.09298165385641	21.53213530636016
22	21.67734468003992	26.196110093357394	29.65524572871828	22.471299497884406
23	22.57096197429762	26.202570971138663	30.382988004761803	20.843479049801914
24	22.627551932169034	26.433833738245553	28.092767207700263	22.845847121885146
25	22.45764459307009	26.26750050174902	29.352867484189176	21.921987420991712
26	22.188303886699114	27.051832735663954	29.388745975697933	21.371117401938996
27	21.46450228788388	27.06635825521475	28.912886289467487	22.556253167433876
28	21.75450863878928	26.667387596764247	29.29036651047533	22.287737253971148
29	22.284575547822868	26.754953110523168	28.65000646087778	22.310464880776184
30	22.022932908595532	26.860022562668224	29.95835712249916	21.158687406237085
31	22.502183410115443	26.752432909970185	29.784188353374276	20.961195326540093
32	22.031639055960365	26.43603318600088	29.027669969198566	22.504657788840184
33	21.702913260195587	26.578859824612373	29.649838752986433	22.068388162205608
34	21.426378526791563	26.887561481438038	29.922386987333933	21.76367300443647
35	20.849664996613768	28.741833404662646	28.960403525348177	21.44809807337541
36	22.886903479984568	26.854798874249326	28.69949403537262	21.55880361039349
37	21.8583867234002	27.25514418754691	28.802089108792934	22.084379980259957
38	21.676932283585796	26.18094306821129	30.050138244455788	22.09198640374713
39	21.230810963880486	26.148134639194343	30.572644551830447	22.048409845094728
40	20.372705586505653	26.77406081289756	30.27539835206377	22.577835248533017
41	21.53680913284023	25.512356772420393	30.451995678085158	22.49883841665422
42	20.369177305731487	27.220640350885233	29.93998256937654	22.470199774006744
43	20.750277451169968	26.654053444747582	29.479014977322777	23.116654126759673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	124.0
1	236.5
2	349.0
3	853.0
4	1357.0
5	1357.0
6	2192.0
7	3027.0
8	3421.0
9	3815.0
10	5738.5
11	7662.0
12	7662.0
13	14746.5
14	21831.0
15	35028.5
16	48226.0
17	44115.0
18	40004.0
19	40004.0
20	42646.5
21	45289.0
22	36761.0
23	28233.0
24	32010.0
25	35787.0
26	35787.0
27	40195.0
28	44603.0
29	49231.5
30	53860.0
31	61140.0
32	68420.0
33	68420.0
34	77106.0
35	85792.0
36	94343.0
37	102894.0
38	109482.0
39	116070.0
40	116070.0
41	119538.0
42	123006.0
43	131100.0
44	139194.0
45	142959.0
46	146724.0
47	146724.0
48	178146.0
49	209568.0
50	200759.0
51	191950.0
52	178905.0
53	165860.0
54	165860.0
55	149480.0
56	133100.0
57	114325.0
58	95550.0
59	85700.5
60	75851.0
61	75851.0
62	67326.5
63	58802.0
64	51065.5
65	43329.0
66	37367.5
67	31406.0
68	31406.0
69	26717.0
70	22028.0
71	18601.0
72	15174.0
73	12553.5
74	9933.0
75	9933.0
76	7925.5
77	5918.0
78	4706.5
79	3495.0
80	2732.0
81	1969.0
82	1969.0
83	1500.0
84	1031.0
85	785.5
86	540.0
87	422.0
88	304.0
89	304.0
90	240.0
91	176.0
92	125.5
93	75.0
94	55.5
95	36.0
96	36.0
97	20.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2182366.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.86101914933815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.41697113856587	43.566450895861806
2	10.431386568895858	11.028274503451055
3	2.9909472799968744	4.743135643277268
4	1.244399555396492	2.6312091490896736
5	0.6545592197894894	1.7300333725834018
6	0.39641676158303646	1.2572996415095703
7	0.2673430195650659	0.9892417133669583
8	0.2009700434502064	0.8498785052211746
9	0.15895761994773194	0.7562395612791248
>10	1.0394732221830294	10.81303770403405
>50	0.1088738962792083	3.975314764504117
>100	0.07839358560722526	8.125796817666185
>500	0.007306765032023884	2.678871413824861
>1k	0.003479411920011372	3.230371306331132
>5k	2.609558940008529E-4	0.8620576942394178
>10k+	2.609558940008529E-4	2.762787313760157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23943	1.0971120334536004	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19567	0.8965957130930376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16575	0.7594968030110439	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8462	0.3877443105326971	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5234	0.23983144898701683	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5052	0.23149187624807205	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4594	0.2105054789160022	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4148	0.19006894352276382	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4024	0.18438703682150473	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3761	0.17233589599544716	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2865	0.1312795378960266	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2590	0.11867853513113748	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2186	0.10016651652380948	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	4.582182823596042E-5	0.0	0.0	0.0
3	0.0	4.582182823596042E-5	0.0	0.0	0.0
4	0.0	9.164365647192084E-5	0.0	4.582182823596042E-5	0.0
5	0.0	9.164365647192084E-5	0.0	4.582182823596042E-5	4.582182823596042E-5
6	0.0	9.164365647192084E-5	0.0	4.582182823596042E-5	9.164365647192084E-5
7	0.0	9.164365647192084E-5	0.0	4.582182823596042E-5	9.164365647192084E-5
8	1.3746548470788125E-4	9.164365647192084E-5	0.0	4.582182823596042E-5	9.164365647192084E-5
9	1.3746548470788125E-4	9.164365647192084E-5	0.0	2.749309694157625E-4	9.164365647192084E-5
10	1.3746548470788125E-4	9.164365647192084E-5	0.0	2.749309694157625E-4	9.164365647192084E-5
11	1.3746548470788125E-4	9.164365647192084E-5	0.0	5.040401105955646E-4	9.164365647192084E-5
12	1.3746548470788125E-4	9.164365647192084E-5	0.0	5.49861938831525E-4	9.164365647192084E-5
13	1.3746548470788125E-4	9.164365647192084E-5	0.0	6.873274235394063E-4	1.3746548470788125E-4
14	1.3746548470788125E-4	9.164365647192084E-5	0.0	8.247929082472876E-4	1.3746548470788125E-4
15	1.3746548470788125E-4	9.164365647192084E-5	0.0	9.622583929551689E-4	1.3746548470788125E-4
16	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.0013288330188428522	1.3746548470788125E-4
17	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.0018328731294384169	1.3746548470788125E-4
18	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.0023369132400339813	1.3746548470788125E-4
19	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.003161706148281269	1.3746548470788125E-4
20	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.004857113793011804	1.3746548470788125E-4
21	1.3746548470788125E-4	1.3746548470788125E-4	0.0	0.008522860051888638	1.3746548470788125E-4
22	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.013654904814316205	1.3746548470788125E-4
23	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.016220927195529988	1.3746548470788125E-4
24	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.020390713565002386	1.3746548470788125E-4
25	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.024514678106238825	1.3746548470788125E-4
26	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.030883912231037324	1.3746548470788125E-4
27	1.3746548470788125E-4	1.8328731294384168E-4	0.0	0.07065725913985096	1.3746548470788125E-4
28	1.3746548470788125E-4	2.291091411798021E-4	0.0	0.15359476824693932	1.3746548470788125E-4
29	1.3746548470788125E-4	2.291091411798021E-4	0.0	0.2608178463190867	1.8328731294384168E-4
30	1.8328731294384168E-4	2.291091411798021E-4	0.0	0.4084557768953512	1.8328731294384168E-4
31	1.8328731294384168E-4	2.291091411798021E-4	0.0	0.5820288622531693	1.8328731294384168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7155	0.0	21.227814	1
ACGTTTA	115	1.3460522E-10	20.913044	26
CGAATTA	90	9.4880306E-8	20.555555	15
TATGTCG	55	5.14513E-4	20.181818	5
GTATTGG	790	0.0	19.905064	1
TATACCG	95	3.6093952E-6	17.526316	5
GGACCGT	375	0.0	16.773333	6
TGTTACG	200	3.6379788E-12	15.725	16
TAATACT	725	0.0	15.565517	4
AGTTCCG	180	2.0190782E-10	15.416666	32
TTGGACC	1205	0.0	15.199171	4
TTATGCG	305	0.0	15.163934	4
TGGACCC	1165	0.0	15.085837	5
ATTGGAC	1025	0.0	14.800001	3
CCGAATT	125	2.9615112E-6	14.800001	14
GAACCGT	75	0.0041059777	14.8	6
CGTTATT	215	1.2732926E-11	14.627907	10
CTAATAC	635	0.0	14.566929	3
CGTGTCT	180	3.3342076E-9	14.388888	35
TACTGGT	605	0.0	14.371901	7
>>END_MODULE