FastQCFastQC Report
Wed 25 May 2016
SRR2088446_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088446_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3450561
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT418521.2129042205021154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT354001.0259201329870709No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT300930.8721190554231616No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143600.4161642121382581No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG112830.326990306793591No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109450.31719479817919466No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA96360.2792589378944467No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG93690.271521065704968No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79780.23120878025341385No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG75690.21935563521409995No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA64270.18625956764711593No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA47150.13664444709135704No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT44390.12864574774942394No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43980.12745753516602085No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA43820.12699384245054643No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC43270.12539989874110327No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG42100.12200914575919684No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37580.10890982654704555No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC37030.10731588283760235No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36710.1063884974066536No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36550.1059248046911792No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34810.1008821464103953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119400.022.838361
GTATTGG21600.018.071761
GTATTAG9000.017.0611111
ATACTGG8350.016.6167666
ATTGGAC24400.016.377053
TAATACT9950.016.1758794
ATTAGAG9100.015.8571423
TTGGACC29550.015.7140454
ACGTTTA1659.767973E-1015.6969726
ACCGTTA4600.015.6847838
TTAACGG6600.015.41666735
TGGACCC29550.015.2758045
TATTAGA9900.015.1363642
TACTGGT9800.014.9132657
TGTTACG3600.014.90277916
ACCGCCT8700.014.88505812
GTATCAA186050.014.6269832
CTAATAC9000.014.5944443
TTATGCG3550.014.5915494
TCTAATA8750.014.5885722
Produced by FastQC (version 0.11.5)