##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088446_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3450561
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.323641575964025	31.0	31.0	34.0	30.0	34.0
2	31.539173485123143	31.0	31.0	34.0	30.0	34.0
3	31.648122145935112	31.0	31.0	34.0	30.0	34.0
4	35.35445975306624	37.0	35.0	37.0	33.0	37.0
5	35.2266095281318	37.0	35.0	37.0	33.0	37.0
6	35.28572687165942	37.0	35.0	37.0	32.0	37.0
7	35.12237923050773	37.0	35.0	37.0	32.0	37.0
8	35.16086543608416	37.0	35.0	37.0	32.0	37.0
9	36.68919604667183	39.0	35.0	39.0	32.0	39.0
10	36.53892627894421	38.0	35.0	39.0	32.0	39.0
11	36.57391740067774	38.0	35.0	39.0	32.0	39.0
12	36.41766947461587	38.0	35.0	39.0	32.0	39.0
13	36.441428799548824	38.0	35.0	39.0	32.0	39.0
14	37.372652157141985	39.0	36.0	41.0	32.0	41.0
15	37.391233193674886	39.0	36.0	41.0	32.0	41.0
16	37.248723903156616	39.0	36.0	40.0	32.0	41.0
17	37.33337361663799	39.0	36.0	40.0	32.0	41.0
18	37.21081673385864	39.0	36.0	40.0	31.0	41.0
19	37.29332940353757	39.0	36.0	40.0	31.0	41.0
20	37.2012304665821	39.0	36.0	40.0	31.0	41.0
21	37.043947346532924	39.0	36.0	40.0	31.0	41.0
22	36.95539305057931	39.0	36.0	40.0	31.0	41.0
23	36.75954083988082	39.0	35.0	40.0	30.0	41.0
24	36.94810264186027	39.0	36.0	40.0	31.0	41.0
25	36.912073717867905	39.0	36.0	40.0	30.0	41.0
26	36.727334192903704	39.0	35.0	40.0	30.0	41.0
27	36.664968971712135	39.0	35.0	40.0	30.0	41.0
28	36.47133234276977	39.0	35.0	40.0	30.0	41.0
29	36.28987692146291	38.0	35.0	40.0	30.0	41.0
30	36.10932396210355	38.0	35.0	40.0	29.0	41.0
31	35.83816660537229	38.0	35.0	40.0	28.0	41.0
32	35.69676640986784	38.0	34.0	40.0	27.0	41.0
33	35.484642352359515	38.0	34.0	40.0	27.0	41.0
34	35.35861675826047	38.0	34.0	40.0	26.0	41.0
35	35.11518416860331	38.0	34.0	40.0	25.0	41.0
36	34.966816120625026	38.0	33.0	40.0	25.0	41.0
37	34.79433721067386	38.0	33.0	40.0	24.0	41.0
38	34.720026685515776	38.0	33.0	40.0	24.0	41.0
39	34.62590546870494	38.0	33.0	40.0	24.0	41.0
40	34.49327225341039	38.0	33.0	40.0	23.0	41.0
41	34.281600586107594	38.0	33.0	40.0	22.0	41.0
42	34.03872037039774	38.0	33.0	40.0	21.0	41.0
43	33.71287654384316	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	10.0
15	21.0
16	64.0
17	148.0
18	334.0
19	777.0
20	1726.0
21	3405.0
22	6102.0
23	10633.0
24	16972.0
25	25465.0
26	37066.0
27	51183.0
28	67839.0
29	88502.0
30	110621.0
31	135047.0
32	162761.0
33	196341.0
34	238498.0
35	288424.0
36	360966.0
37	475135.0
38	599975.0
39	572539.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.852324882823396	21.09929370905195	13.57950199981974	23.46887940830491
2	19.143785604717607	23.446622157962143	34.80529687781204	22.604295359508207
3	19.842164795811463	24.82749906464485	32.37302571958588	22.9573104199578
4	13.84183615359937	16.030349847459586	36.2482796275736	33.879534371367434
5	12.601400178115965	38.558193870503956	34.960170244780485	13.880235706599594
6	32.66503620715588	37.20221146648328	13.257641293691083	16.87511103266976
7	26.46627606351547	32.66703588199136	21.26749824159028	19.599189812902885
8	27.470170792517507	33.96424523432566	18.74176402040132	19.82381995275551
9	24.692767350004825	14.263622639912757	21.38176951516	39.661840494922416
10	16.568899955688366	27.18891217978758	33.32394355584498	22.918244308679082
11	33.4796283850655	22.10854988507666	23.476878107646844	20.934943622210998
12	21.62836709740822	26.86325498955097	30.585287435869123	20.923090477171684
13	31.922200476965916	20.621400404166163	26.021565768580817	21.434833350287096
14	20.920366282468272	22.448088875982776	27.332135267279728	29.299409574269227
15	22.9363573053773	29.16905395963149	25.34390784570973	22.550680889281484
16	22.40754474417348	27.864773293386207	26.15826817726161	23.5694137851787
17	22.226356815601868	26.2037100633781	26.57208494502778	24.997848175992253
18	23.581237949423297	25.072618626362498	29.160736471547672	22.185406952666536
19	23.213587587641545	24.13201795302271	30.181324138306785	22.473070321028956
20	24.19991995504499	23.95111983239827	30.07328373560125	21.775676476955486
21	23.108677110765466	24.828455430870516	30.478319322568126	21.584548135795888
22	21.725713586863122	25.236418078103824	30.083658860109995	22.95420947492306
23	22.82808505631403	25.461251083519464	31.044604051341217	20.666059808825292
24	22.82782422916158	25.48800035704339	28.334204206214586	23.34997120758045
25	22.66364802708893	25.65310394454699	29.544123404860834	22.13912462350325
26	22.545058615106356	26.340586356827195	29.578233800242913	21.536121227823536
27	21.453902713210983	26.538786011897773	29.05327568473648	22.95403559015476
28	21.973789189641916	26.00435117651883	29.280021422603454	22.741838211235795
29	22.383374761379383	25.940593428141106	28.74949899451133	22.926532815968187
30	22.143355819531955	26.106682362665083	30.417807423198724	21.33215439460424
31	22.88468454839662	25.79852957243764	30.30185526353541	21.014930615630327
32	22.3524812342109	25.56619054119026	29.13091523378372	22.950412990815117
33	22.054152933392572	25.755985765792865	29.787156349358845	22.40270495145572
34	21.431616482073494	26.49186610525071	30.156400654850035	21.920116757825756
35	20.89497910629605	28.704897551441633	28.956074099255165	21.44404924300715
36	23.255696682365564	26.14015518056339	28.927875785995376	21.676272351075664
37	22.0951027963279	26.906059623348206	28.817082207791717	22.181755372532177
38	21.96793506910905	25.38381440003524	30.21360874362169	22.434641787234018
39	21.56898544903278	25.226419703926407	30.939896440028157	22.264698407012656
40	20.365644890787323	26.0764553937751	30.525007382857456	23.032892332580126
41	22.015028860524417	24.749453784471566	30.531991754384286	22.703525600619727
42	20.50950555576325	26.832564327945512	30.087658209780958	22.570271906510275
43	20.958765835468494	26.514094374798763	29.195745271565986	23.331394518166757
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	197.0
1	378.5
2	560.0
3	1452.5
4	2345.0
5	2345.0
6	3671.5
7	4998.0
8	5706.0
9	6414.0
10	9732.5
11	13051.0
12	13051.0
13	24585.5
14	36120.0
15	58995.5
16	81871.0
17	75114.5
18	68358.0
19	68358.0
20	71890.5
21	75423.0
22	59160.5
23	42898.0
24	48103.0
25	53308.0
26	53308.0
27	58167.5
28	63027.0
29	70007.5
30	76988.0
31	85301.5
32	93615.0
33	93615.0
34	104855.5
35	116096.0
36	128503.0
37	140910.0
38	151417.0
39	161924.0
40	161924.0
41	169662.0
42	177400.0
43	189796.5
44	202193.0
45	214559.0
46	226925.0
47	226925.0
48	296725.0
49	366525.0
50	349826.5
51	333128.0
52	309940.5
53	286753.0
54	286753.0
55	255440.0
56	224127.0
57	189335.5
58	154544.0
59	137824.0
60	121104.0
61	121104.0
62	107579.5
63	94055.0
64	82148.0
65	70241.0
66	60518.0
67	50795.0
68	50795.0
69	43892.5
70	36990.0
71	31646.5
72	26303.0
73	21843.5
74	17384.0
75	17384.0
76	14052.0
77	10720.0
78	8488.0
79	6256.0
80	4846.0
81	3436.0
82	3436.0
83	2603.5
84	1771.0
85	1344.5
86	918.0
87	693.0
88	468.0
89	468.0
90	356.0
91	244.0
92	180.0
93	116.0
94	78.5
95	41.0
96	41.0
97	29.5
98	18.0
99	10.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3450561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.39132807176124
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4307341245867	35.60075183558195
2	11.853659103140796	10.76106658402966
3	3.9327935578591795	5.3554416786988535
4	1.797842355095318	3.264258086457578
5	0.9335647469121902	2.1187871851660987
6	0.5701573596003139	1.5528119857288196
7	0.37366991033541336	1.1872961443406247
8	0.2790110749229025	1.0131746589984176
9	0.20067680421056336	0.819808799068285
>10	1.3775560599333756	12.079109701818888
>50	0.14129127276333536	4.422540301161217
>100	0.09341667365128122	8.460247937639595
>500	0.008902241891392207	2.784985825925408
>1k	0.0060202211347044135	5.2041733566754775
>5k	3.2022452844172417E-4	1.1912936759257746
>10k+	3.84269434130069E-4	4.184252242783487
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41852	1.2129042205021154	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35400	1.0259201329870709	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	30093	0.8721190554231616	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14360	0.4161642121382581	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11283	0.326990306793591	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10945	0.31719479817919466	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9636	0.2792589378944467	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9369	0.271521065704968	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7978	0.23120878025341385	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7569	0.21935563521409995	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6427	0.18625956764711593	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4715	0.13664444709135704	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4439	0.12864574774942394	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4398	0.12745753516602085	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4382	0.12699384245054643	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4327	0.12539989874110327	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4210	0.12200914575919684	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3758	0.10890982654704555	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3703	0.10731588283760235	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3671	0.1063884974066536	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3655	0.1059248046911792	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3481	0.1008821464103953	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	2.8980794717148894E-5	0.0	0.0	0.0	0.0
6	5.796158943429779E-5	0.0	0.0	0.0	2.8980794717148894E-5
7	5.796158943429779E-5	0.0	0.0	0.0	2.8980794717148894E-5
8	1.1592317886859558E-4	0.0	0.0	0.0	2.8980794717148894E-5
9	1.1592317886859558E-4	0.0	0.0	8.694238415144668E-5	2.8980794717148894E-5
10	1.1592317886859558E-4	0.0	0.0	1.7388476830289336E-4	2.8980794717148894E-5
11	1.4490397358574446E-4	0.0	0.0	2.898079471714889E-4	2.8980794717148894E-5
12	1.4490397358574446E-4	0.0	0.0	3.477695366057867E-4	2.8980794717148894E-5
13	1.4490397358574446E-4	0.0	0.0	3.477695366057867E-4	2.8980794717148894E-5
14	1.4490397358574446E-4	0.0	0.0	4.926735101915312E-4	2.8980794717148894E-5
15	1.4490397358574446E-4	0.0	0.0	7.535006626458712E-4	2.8980794717148894E-5
16	1.4490397358574446E-4	0.0	0.0	0.0012751549675545514	2.8980794717148894E-5
17	1.4490397358574446E-4	0.0	0.0	0.0017678284777460824	2.8980794717148894E-5
18	1.4490397358574446E-4	0.0	0.0	0.002405405961523358	2.8980794717148894E-5
19	1.4490397358574446E-4	0.0	0.0	0.0036225993396436116	2.8980794717148894E-5
20	1.7388476830289336E-4	0.0	0.0	0.005883101327581225	2.8980794717148894E-5
21	1.7388476830289336E-4	0.0	0.0	0.010288182124587857	2.8980794717148894E-5
22	1.7388476830289336E-4	0.0	0.0	0.015330840405371764	2.8980794717148894E-5
23	1.7388476830289336E-4	0.0	0.0	0.01933019007633831	2.8980794717148894E-5
24	1.7388476830289336E-4	0.0	0.0	0.02463367550957656	2.8980794717148894E-5
25	1.7388476830289336E-4	0.0	0.0	0.02935754504847183	2.8980794717148894E-5
26	2.0286556302004225E-4	0.0	0.0	0.03677662849606195	2.8980794717148894E-5
27	2.0286556302004225E-4	0.0	0.0	0.0814360331551884	2.8980794717148894E-5
28	2.0286556302004225E-4	0.0	0.0	0.17411661466063055	2.8980794717148894E-5
29	2.0286556302004225E-4	0.0	0.0	0.290793294191872	2.8980794717148894E-5
30	2.0286556302004225E-4	0.0	0.0	0.44720264328032455	2.8980794717148894E-5
31	2.0286556302004225E-4	0.0	0.0	0.628680379799111	2.8980794717148894E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11940	0.0	22.83836	1
GTATTGG	2160	0.0	18.07176	1
GTATTAG	900	0.0	17.061111	1
ATACTGG	835	0.0	16.616766	6
ATTGGAC	2440	0.0	16.37705	3
TAATACT	995	0.0	16.175879	4
ATTAGAG	910	0.0	15.857142	3
TTGGACC	2955	0.0	15.714045	4
ACGTTTA	165	9.767973E-10	15.69697	26
ACCGTTA	460	0.0	15.684783	8
TTAACGG	660	0.0	15.416667	35
TGGACCC	2955	0.0	15.275804	5
TATTAGA	990	0.0	15.136364	2
TACTGGT	980	0.0	14.913265	7
TGTTACG	360	0.0	14.902779	16
ACCGCCT	870	0.0	14.885058	12
GTATCAA	18605	0.0	14.626983	2
CTAATAC	900	0.0	14.594444	3
TTATGCG	355	0.0	14.591549	4
TCTAATA	875	0.0	14.588572	2
>>END_MODULE