FastQCFastQC Report
Wed 25 May 2016
SRR2088445_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088445_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3155851
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT419331.3287382705964255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT344281.0909260291439615No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT282310.8945606113850115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152210.48231047663530374No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG91930.2913001912954699No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA85310.27032328205609196No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG80300.2544480078432093No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA75200.23828754906362817No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA70480.2233312028989962No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG63940.20260779105223917No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46810.14832766185729301No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA46220.146458118586714No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38600.12231249193957508No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC38270.12126681519501396No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37370.11841496952802903No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA37360.11838328235395144No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35300.11185572449396375No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG34420.10906725317513406No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC32670.10352199771155228No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119000.020.8163851
TATACCG2050.018.9512185
GTATTGG14550.017.03781
TCTATAC3150.016.4444453
GTATACG1504.6802597E-916.0333331
ACCGTTA5700.015.5789478
AATACTG8300.015.1566275
CGAACTA6350.014.85826824
TAATACT8900.014.7584284
CCGTTTA5650.014.73451227
TGGACCC19950.014.5588975
TTCGTTA3350.014.35820930
GTTTAGG4550.014.230771
TTGGACC21350.014.21077254
ATTGGAC18600.014.0241933
CGTTTAC5950.013.99159728
GGACCGT7600.013.8756
CCTATCG800.006301835613.87513
TTAACGG4550.013.82417735
TACTGGT7950.013.7295597
Produced by FastQC (version 0.11.5)