##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088445_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3155851
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.272925432791347	31.0	31.0	33.0	30.0	34.0
2	31.495668838611202	31.0	31.0	34.0	30.0	34.0
3	31.599978579470324	31.0	31.0	34.0	30.0	34.0
4	35.311888932652394	37.0	35.0	37.0	33.0	37.0
5	35.189256717126376	37.0	35.0	37.0	33.0	37.0
6	35.24917114274407	37.0	35.0	37.0	32.0	37.0
7	35.080565907579285	37.0	35.0	37.0	32.0	37.0
8	35.13286939085527	37.0	35.0	37.0	32.0	37.0
9	36.64995495668205	39.0	35.0	39.0	32.0	39.0
10	36.49993488285727	38.0	35.0	39.0	32.0	39.0
11	36.52911908705449	38.0	35.0	39.0	32.0	39.0
12	36.38611011736612	38.0	35.0	39.0	32.0	39.0
13	36.40437777322187	38.0	35.0	39.0	32.0	39.0
14	37.32453179823762	39.0	36.0	41.0	32.0	41.0
15	37.34412873104592	39.0	36.0	41.0	32.0	41.0
16	37.19642910897885	39.0	36.0	40.0	31.0	41.0
17	37.29342988626522	39.0	36.0	40.0	32.0	41.0
18	37.168894539064105	39.0	36.0	40.0	31.0	41.0
19	37.26503532644602	39.0	36.0	40.0	31.0	41.0
20	37.17385358180725	39.0	36.0	40.0	31.0	41.0
21	37.02249821046684	39.0	36.0	40.0	31.0	41.0
22	36.93460686198429	39.0	36.0	40.0	31.0	41.0
23	36.736277156304276	39.0	35.0	40.0	30.0	41.0
24	36.9170521675453	39.0	36.0	40.0	31.0	41.0
25	36.88879734816378	39.0	36.0	40.0	30.0	41.0
26	36.6979071572137	39.0	35.0	40.0	30.0	41.0
27	36.63151080326669	39.0	35.0	40.0	30.0	41.0
28	36.43399197237132	38.0	35.0	40.0	30.0	41.0
29	36.25113289569121	38.0	35.0	40.0	30.0	41.0
30	36.06261607407954	38.0	35.0	40.0	29.0	41.0
31	35.783900127097255	38.0	34.0	40.0	28.0	41.0
32	35.63646224108806	38.0	34.0	40.0	27.0	41.0
33	35.415945176118896	38.0	34.0	40.0	27.0	41.0
34	35.298906063689316	38.0	34.0	40.0	26.0	41.0
35	35.05619625261142	38.0	33.0	40.0	25.0	41.0
36	34.90265731810532	38.0	33.0	40.0	25.0	41.0
37	34.72226762290108	38.0	33.0	40.0	24.0	41.0
38	34.63643499011835	38.0	33.0	40.0	24.0	41.0
39	34.52742002078045	38.0	33.0	40.0	24.0	41.0
40	34.390137240319646	38.0	33.0	40.0	23.0	41.0
41	34.15836489111812	38.0	33.0	40.0	22.0	41.0
42	33.91872968654097	37.0	33.0	40.0	21.0	41.0
43	33.57564473100916	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	0.0
14	4.0
15	14.0
16	41.0
17	118.0
18	315.0
19	777.0
20	1638.0
21	3190.0
22	5841.0
23	9875.0
24	15948.0
25	23928.0
26	34353.0
27	47647.0
28	63541.0
29	82012.0
30	102260.0
31	125435.0
32	150415.0
33	183137.0
34	220742.0
35	265175.0
36	333912.0
37	438647.0
38	546489.0
39	500394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.28154339352523	21.76164210540992	13.995020677465444	22.961793823599404
2	19.324961793189857	23.925178977080982	34.55882422839355	22.191035001335617
3	19.98963195664181	25.18563138754016	32.469783902978946	22.35495275283909
4	13.983676669145659	16.595491992492676	36.16035738062412	33.260473957737545
5	12.499259312305936	39.06692679724107	34.709655177002965	13.724158713450032
6	32.28688553420297	37.51539600570496	13.446642442878323	16.75107601721374
7	26.05103979877377	32.976778688220705	21.51676362413815	19.455417888867377
8	27.326702052790196	34.32684242697136	18.767552713990614	19.578902806247825
9	24.579392373087323	14.402454361755353	21.765444566299234	39.25270869885809
10	16.695338278011224	27.596043032449884	33.1354363688273	22.573182320711595
11	33.14250260864661	22.628983434262263	23.418659499450385	20.809854457640746
12	21.183509614363924	27.451010836696664	30.735069558100175	20.630409990839237
13	31.543377681645936	20.948961151841452	26.311920302954732	21.19574086355788
14	20.567352514424794	23.001941473155735	27.75818630220502	28.672519710214456
15	22.744324747904766	29.533396855554965	25.68977432711494	22.032504069425332
16	21.836994205366477	28.189924049012454	26.706742491961755	23.266339253659314
17	21.81478149633807	26.660257407589903	27.093547825927146	24.431413270144883
18	22.85247307303165	25.53013434411194	29.608178586378127	22.009213996478287
19	22.8276303285548	24.712922124650373	30.72283830890622	21.736609237888608
20	23.636287011015412	24.495326300259425	30.59029085973958	21.278095828985588
21	22.483856176986812	25.35221086166616	31.134930007785538	21.029002953561495
22	21.35005740131584	25.79047616633358	30.540320186219184	22.319146246131393
23	22.19350660091367	26.048092891584552	31.49001648049924	20.268384027002543
24	22.23165795850311	26.145942885136215	28.982737144434257	22.63966201192642
25	22.09480105366191	26.214830801580934	30.14188565936731	21.548482485389837
26	21.91358210511206	26.863879188212625	30.212548057560383	21.00999064911493
27	20.975546690892568	26.94243169275102	29.71344337866395	22.368578237692464
28	21.34093149518149	26.614437753873677	29.976003303071025	22.068627447873805
29	21.87752210101174	26.51402109922173	29.328792772535838	22.279664027230687
30	21.53517387227724	26.690613720356254	30.987045966365333	20.787166441001176
31	22.212360469489845	26.466965645716478	30.794990004280937	20.525683880512737
32	21.725740537179988	26.198828778671746	29.76550540567346	22.309925278474807
33	21.36399975790999	26.438130317305852	30.330709529695792	21.867160395088362
34	20.94233853245923	26.858302245574965	30.701322717707523	21.49803650425828
35	20.396812143539094	28.94236768465938	29.650164092030963	21.01065607977056
36	22.607753027630263	26.60366411468729	29.554278703272114	21.23430415441033
37	21.480576871341515	27.21494138981847	29.51397261784539	21.79050912099462
38	21.382219883004616	25.867190814775476	30.815428231560997	21.93516107065891
39	20.89902216549514	25.730999340589904	31.47686630325703	21.893112190657924
40	19.841906351091986	26.56706542862765	31.05102237082803	22.540005849452335
41	21.253886827990296	25.3394092433388	31.093863430180956	22.312840498489948
42	20.00468336432867	27.27657294339942	30.44754014051994	22.271203551751967
43	20.389714216545713	26.94205144666209	29.80343495304436	22.86479938374784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	175.0
1	396.0
2	617.0
3	1482.5
4	2348.0
5	2348.0
6	3788.5
7	5229.0
8	6014.0
9	6799.0
10	10127.5
11	13456.0
12	13456.0
13	25944.0
14	38432.0
15	61476.5
16	84521.0
17	76267.5
18	68014.0
19	68014.0
20	71720.0
21	75426.0
22	59050.5
23	42675.0
24	47263.0
25	51851.0
26	51851.0
27	56609.5
28	61368.0
29	67596.5
30	73825.0
31	82110.0
32	90395.0
33	90395.0
34	101978.0
35	113561.0
36	125794.0
37	138027.0
38	147413.5
39	156800.0
40	156800.0
41	162278.0
42	167756.0
43	180497.0
44	193238.0
45	203854.5
46	214471.0
47	214471.0
48	272368.5
49	330266.0
50	312277.5
51	294289.0
52	271504.0
53	248719.0
54	248719.0
55	220194.5
56	191670.0
57	162359.0
58	133048.0
59	117597.5
60	102147.0
61	102147.0
62	90031.5
63	77916.0
64	67757.0
65	57598.0
66	49511.5
67	41425.0
68	41425.0
69	35365.5
70	29306.0
71	24608.5
72	19911.0
73	16509.0
74	13107.0
75	13107.0
76	10540.5
77	7974.0
78	6196.0
79	4418.0
80	3459.0
81	2500.0
82	2500.0
83	1867.0
84	1234.0
85	947.5
86	661.0
87	508.0
88	355.0
89	355.0
90	283.5
91	212.0
92	134.5
93	57.0
94	46.5
95	36.0
96	36.0
97	23.5
98	11.0
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3155851.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.944325179545636
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.15815541049184	36.36868032792647
2	11.61284102803519	10.670882889008356
3	3.8239946928453845	5.270725669588352
4	1.6302903128634867	2.9961035308505286
5	0.8736954490336823	2.0070673909146324
6	0.5448730919461588	1.5020295910775276
7	0.3619451414014165	1.1640527691592313
8	0.2590042124268615	0.9519819006889474
9	0.2073058581047089	0.8572074980748742
>10	1.2648979401948222	11.296105237181358
>50	0.1455564515127384	4.649414977947161
>100	0.10153116688163362	9.224516485773352
>500	0.009822229066260723	3.1352219399392838
>1k	0.005395308923694572	4.613721851854975
>5k	4.150237633611209E-4	1.4846334476622125
>10k+	2.766825089074139E-4	3.807654492352784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41933	1.3287382705964255	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34428	1.0909260291439615	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28231	0.8945606113850115	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15221	0.48231047663530374	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	9193	0.2913001912954699	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8531	0.27032328205609196	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8030	0.2544480078432093	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	7520	0.23828754906362817	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7048	0.2233312028989962	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6394	0.20260779105223917	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4681	0.14832766185729301	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4622	0.146458118586714	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3860	0.12231249193957508	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3827	0.12126681519501396	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3737	0.11841496952802903	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3736	0.11838328235395144	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3530	0.11185572449396375	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3442	0.10906725317513406	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3267	0.10352199771155228	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.1687174077610125E-5	0.0	0.0	0.0	0.0
4	3.1687174077610125E-5	0.0	0.0	0.0	0.0
5	3.1687174077610125E-5	3.1687174077610125E-5	0.0	0.0	0.0
6	3.1687174077610125E-5	3.1687174077610125E-5	0.0	0.0	0.0
7	3.1687174077610125E-5	3.1687174077610125E-5	0.0	0.0	0.0
8	6.337434815522025E-5	3.1687174077610125E-5	0.0	0.0	0.0
9	9.506152223283039E-5	3.1687174077610125E-5	0.0	1.267486963104405E-4	0.0
10	9.506152223283039E-5	3.1687174077610125E-5	0.0	2.218102185432709E-4	0.0
11	1.267486963104405E-4	3.1687174077610125E-5	0.0	4.1193326300893165E-4	0.0
12	1.267486963104405E-4	3.1687174077610125E-5	0.0	5.703691333969823E-4	0.0
13	1.267486963104405E-4	3.1687174077610125E-5	0.0	6.020563074745925E-4	0.0
14	1.267486963104405E-4	3.1687174077610125E-5	0.0	7.604921778626431E-4	0.0
15	1.267486963104405E-4	3.1687174077610125E-5	0.0	8.238665260178633E-4	0.0
16	1.267486963104405E-4	3.1687174077610125E-5	0.0	0.0012991741371820153	0.0
17	1.267486963104405E-4	6.337434815522025E-5	0.0	0.001742794574268557	0.0
18	1.5843587038805065E-4	6.337434815522025E-5	0.0	0.0023765380558207596	0.0
19	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.003295466104071453	0.0
20	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.0059888759006683145	0.0
21	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.011090510927163545	0.0
22	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.018093376398315382	0.0
23	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.022814765335879294	0.0
24	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.02969088211072069	0.0
25	1.9012304446566078E-4	6.337434815522025E-5	0.0	0.034919265833526364	0.0
26	2.218102185432709E-4	6.337434815522025E-5	0.0	0.04467891544943028	0.0
27	2.218102185432709E-4	6.337434815522025E-5	0.0	0.09287510722147528	0.0
28	2.53497392620881E-4	6.337434815522025E-5	0.0	0.19487612057730228	0.0
29	2.53497392620881E-4	6.337434815522025E-5	0.0	0.3275186312661783	0.0
30	2.53497392620881E-4	6.337434815522025E-5	0.0	0.5056322367564248	0.0
31	2.53497392620881E-4	6.337434815522025E-5	0.0	0.7142605908834099	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11900	0.0	20.816385	1
TATACCG	205	0.0	18.951218	5
GTATTGG	1455	0.0	17.0378	1
TCTATAC	315	0.0	16.444445	3
GTATACG	150	4.6802597E-9	16.033333	1
ACCGTTA	570	0.0	15.578947	8
AATACTG	830	0.0	15.156627	5
CGAACTA	635	0.0	14.858268	24
TAATACT	890	0.0	14.758428	4
CCGTTTA	565	0.0	14.734512	27
TGGACCC	1995	0.0	14.558897	5
TTCGTTA	335	0.0	14.358209	30
GTTTAGG	455	0.0	14.23077	1
TTGGACC	2135	0.0	14.2107725	4
ATTGGAC	1860	0.0	14.024193	3
CGTTTAC	595	0.0	13.991597	28
GGACCGT	760	0.0	13.875	6
CCTATCG	80	0.0063018356	13.875	13
TTAACGG	455	0.0	13.824177	35
TACTGGT	795	0.0	13.729559	7
>>END_MODULE