FastQCFastQC Report
Wed 25 May 2016
SRR2088444_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088444_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2588351
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT321011.2402104660457567No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT263211.016902267119104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT213540.8250040276608543No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111430.43050575443593236No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67880.262251912511093No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG63230.2442868065420803No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA63120.24386182554066277No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58220.22493085365933754No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA53490.20665667059838483No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG47960.1852917166180321No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35980.13900742210001657No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA35320.13645753609151157No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA30440.11760383348317133No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC29710.11478350501921879No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27600.10663159671930121No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA27480.1061679810813912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.021.3092211
ACCGTTA4000.019.88758
CCGTTAT4350.018.7126439
GGACCGT5050.017.2178236
TGTTACG2600.017.07692316
GTATTAG7000.016.9142861
GTATTGG11200.016.8482151
CCGTTTA4150.016.49397727
ATTAGAC2150.016.3488373
TATACCG1251.6599552E-716.285
GACCGTT4800.016.18757
CGTTATT2800.015.85714310
ATTAGAG6700.015.7388063
TTCGCTA3450.015.55072526
CGCCGTT4650.015.51612925
TAATACT7000.015.3285724
TATGTCG855.3673727E-415.2352945
CGTTTAC4400.015.13636428
TCGCTAC3700.015.027
ATTGGAC13850.014.6931413
Produced by FastQC (version 0.11.5)