##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088444_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2588351
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23328945726449	31.0	31.0	33.0	30.0	34.0
2	31.45459174586445	31.0	31.0	34.0	30.0	34.0
3	31.558154593407153	31.0	31.0	34.0	30.0	34.0
4	35.28175235893432	37.0	35.0	37.0	33.0	37.0
5	35.1376285519236	35.0	35.0	37.0	32.0	37.0
6	35.20448810845206	37.0	35.0	37.0	32.0	37.0
7	35.03799677864401	37.0	35.0	37.0	32.0	37.0
8	35.07729786261601	37.0	35.0	37.0	32.0	37.0
9	36.595281706383716	39.0	35.0	39.0	32.0	39.0
10	36.425531931333886	38.0	35.0	39.0	32.0	39.0
11	36.46473295159737	38.0	35.0	39.0	32.0	39.0
12	36.31513191217111	38.0	35.0	39.0	32.0	39.0
13	36.340606432435166	38.0	35.0	39.0	32.0	39.0
14	37.25468609164677	39.0	36.0	40.0	32.0	41.0
15	37.27358963293618	39.0	36.0	40.0	32.0	41.0
16	37.12944534956812	39.0	36.0	40.0	31.0	41.0
17	37.21220151362779	39.0	36.0	40.0	31.0	41.0
18	37.08974207903024	39.0	36.0	40.0	31.0	41.0
19	37.177008064207676	39.0	36.0	40.0	31.0	41.0
20	37.08163073709864	39.0	36.0	40.0	31.0	41.0
21	36.928321158915466	39.0	36.0	40.0	31.0	41.0
22	36.83318259385995	39.0	36.0	40.0	30.0	41.0
23	36.64137514579746	39.0	35.0	40.0	30.0	41.0
24	36.831771656935246	39.0	35.0	40.0	30.0	41.0
25	36.79938153673903	39.0	35.0	40.0	30.0	41.0
26	36.59566921178774	39.0	35.0	40.0	30.0	41.0
27	36.52716459243742	39.0	35.0	40.0	30.0	41.0
28	36.330980999099424	38.0	35.0	40.0	30.0	41.0
29	36.14368646292562	38.0	35.0	40.0	29.0	41.0
30	35.96033227332769	38.0	35.0	40.0	29.0	41.0
31	35.68120204717212	38.0	34.0	40.0	27.0	41.0
32	35.53540381501581	38.0	34.0	40.0	27.0	41.0
33	35.32580473050216	38.0	34.0	40.0	27.0	41.0
34	35.199760387984476	38.0	34.0	40.0	26.0	41.0
35	34.951057256144935	38.0	33.0	40.0	25.0	41.0
36	34.80756628447996	38.0	33.0	40.0	25.0	41.0
37	34.62841361160059	38.0	33.0	40.0	24.0	41.0
38	34.54696947979621	38.0	33.0	40.0	24.0	41.0
39	34.45246181835462	38.0	33.0	40.0	23.0	41.0
40	34.318207615582274	38.0	33.0	40.0	23.0	41.0
41	34.10129769880515	38.0	33.0	40.0	22.0	41.0
42	33.869868112941404	37.0	33.0	40.0	21.0	41.0
43	33.52790444572626	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	2.0
14	10.0
15	13.0
16	53.0
17	131.0
18	286.0
19	662.0
20	1373.0
21	2789.0
22	5076.0
23	8627.0
24	13698.0
25	20528.0
26	29210.0
27	40413.0
28	53573.0
29	68942.0
30	86536.0
31	104880.0
32	126076.0
33	152893.0
34	183463.0
35	220540.0
36	274387.0
37	357130.0
38	447198.0
39	389857.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.15400113817639	21.6567613897806	13.875088811370636	23.314148660672373
2	19.402932600717598	23.825632613196586	34.40387335411619	22.36756143196962
3	19.93091354302411	25.04895974309512	32.39228373586117	22.627842978019597
4	14.114237211259214	16.64677626797911	36.11198790272262	33.12699861803905
5	12.554672839966448	38.86741017736775	34.871931975222836	13.705985007442964
6	32.40256054916817	37.61611157064865	13.520191040550527	16.461136839632644
7	26.327882114906366	32.816260236729875	21.497741225977464	19.3581164223863
8	27.313413057193557	34.34758268874662	18.81267262438518	19.526331629674644
9	24.693443818091133	14.357751325071444	21.530773840178554	39.418031016658865
10	16.636538089308598	27.619051666485728	33.22914859692522	22.51526164728045
11	33.37966914070001	22.58696753261053	23.270298348253384	20.763064978436077
12	21.234407543644583	27.355099829968964	30.639546182105903	20.770946444280547
13	31.52022272095245	20.928846203625397	26.010034960482564	21.54089611493959
14	20.73868652280931	22.849683060759535	27.556193112912432	28.855437303518727
15	22.951253520098316	29.59239299461317	25.2293834955151	22.226969989773412
16	22.042103254156796	28.185899053103697	26.352646916898053	23.41935077584145
17	22.00485946457803	26.771678184295716	26.960099306469644	24.263363044656614
18	23.062637177106197	25.517327441293702	29.283199998763692	22.13683538283641
19	23.004414779911997	24.786823734493506	30.30230444016287	21.90645704543163
20	23.692768098298878	24.638466730362303	30.205563310385646	21.46320186095317
21	22.67350911835373	25.45164855925645	30.597357159056095	21.277485163333722
22	21.607424958979674	25.8753932523062	30.06280832854586	22.45437346016827
23	22.414850227036442	26.12153452139992	30.83264209529542	20.63097315626822
24	22.407046030464954	26.267032562430675	28.507107420902344	22.818813986202027
25	22.330201738481374	26.149467363583994	29.714555715202458	21.805775182732173
26	22.062425072951854	26.936763986028172	29.696281532141505	21.304529408878473
27	21.243061702218906	26.92389865207617	29.25623302249193	22.576806623213002
28	21.637289532988376	26.60562651665095	29.554376512304554	22.20270743805612
29	22.09406684023921	26.513482908616336	28.949705816560428	22.44274443458403
30	21.910706855445802	26.696688354863774	30.424930776390063	20.96767401330036
31	22.4439034736788	26.423927821226716	30.247018275342104	20.88515042975238
32	21.89490528912037	26.280670589112525	29.409612529367152	22.41481159239995
33	21.615847309735038	26.39232468857585	29.870253300267237	22.12157470142187
34	21.16664239123674	26.897356656805822	30.303540748530626	21.632460203426813
35	20.737875195442967	28.819893437945627	29.2130781335298	21.2291532330816
36	22.800578437777567	26.6537652737206	29.12557068187429	21.42008560662754
37	21.7144429020639	27.177998656287343	29.148481021314343	21.959077420334413
38	21.61356786618198	25.85750541561017	30.349129619591782	22.17979709861607
39	21.081298479224802	25.817364028294463	31.07580849737922	22.025528995101514
40	20.24037698132904	26.53987036534071	30.621001556589505	22.59875109674074
41	21.52810805026057	25.35985266295027	30.695218693291597	22.416820593497558
42	20.276191289357588	27.15010444873976	30.182112086034703	22.391592175867956
43	20.67455302623176	26.705226609528616	29.589186319784293	23.031034044455332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	145.0
1	316.0
2	487.0
3	1054.5
4	1622.0
5	1622.0
6	2688.5
7	3755.0
8	4379.5
9	5004.0
10	7537.0
11	10070.0
12	10070.0
13	19409.0
14	28748.0
15	46768.0
16	64788.0
17	58620.5
18	52453.0
19	52453.0
20	55726.5
21	59000.0
22	46977.5
23	34955.0
24	39001.5
25	43048.0
26	43048.0
27	47269.5
28	51491.0
29	57304.0
30	63117.0
31	70190.5
32	77264.0
33	77264.0
34	86705.5
35	96147.0
36	105360.5
37	114574.0
38	122263.5
39	129953.0
40	129953.0
41	134006.5
42	138060.0
43	147577.5
44	157095.0
45	164974.5
46	172854.0
47	172854.0
48	216128.0
49	259402.0
50	246908.0
51	234414.0
52	216212.0
53	198010.0
54	198010.0
55	177490.5
56	156971.0
57	134842.0
58	112713.0
59	100646.5
60	88580.0
61	88580.0
62	78822.0
63	69064.0
64	60173.5
65	51283.0
66	44794.5
67	38306.0
68	38306.0
69	32867.0
70	27428.0
71	23122.0
72	18816.0
73	15422.0
74	12028.0
75	12028.0
76	9727.5
77	7427.0
78	5869.5
79	4312.0
80	3338.0
81	2364.0
82	2364.0
83	1812.5
84	1261.0
85	971.0
86	681.0
87	507.5
88	334.0
89	334.0
90	269.0
91	204.0
92	139.0
93	74.0
94	55.0
95	36.0
96	36.0
97	22.0
98	8.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2588351.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.20313509202817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.82917075228042	39.77048607901067
2	10.887600813405097	10.714081873000936
3	3.3827851789530863	4.993309084420182
4	1.4279082673061954	2.8103025350116244
5	0.7965795024294219	1.9597104434787713
6	0.48733709565767686	1.4387107771800798
7	0.31933504740902496	1.0998599834099834
8	0.24431606207881915	0.9616892966093185
9	0.18491237885973003	0.8188441881501233
>10	1.2089349742696103	11.636089361027867
>50	0.13044391391010896	4.3939122916879
>100	0.08794042523491505	8.43034731569794
>500	0.007752097168879592	2.5604292909370026
>1k	0.004271563746116996	3.6827991239192337
>5k	3.9551516167749966E-4	1.1907543319437874
>10k+	3.164121293419997E-4	3.5386740245145187
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32101	1.2402104660457567	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	26321	1.016902267119104	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21354	0.8250040276608543	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11143	0.43050575443593236	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6788	0.262251912511093	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6323	0.2442868065420803	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6312	0.24386182554066277	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5822	0.22493085365933754	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5349	0.20665667059838483	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4796	0.1852917166180321	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3598	0.13900742210001657	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3532	0.13645753609151157	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3044	0.11760383348317133	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2971	0.11478350501921879	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2760	0.10663159671930121	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2748	0.1061679810813912	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.863463649250044E-5
2	0.0	0.0	0.0	0.0	3.863463649250044E-5
3	0.0	0.0	0.0	0.0	3.863463649250044E-5
4	0.0	0.0	0.0	0.0	3.863463649250044E-5
5	0.0	0.0	0.0	0.0	3.863463649250044E-5
6	0.0	0.0	0.0	0.0	3.863463649250044E-5
7	0.0	0.0	0.0	0.0	3.863463649250044E-5
8	3.863463649250044E-5	0.0	0.0	0.0	3.863463649250044E-5
9	3.863463649250044E-5	0.0	0.0	3.863463649250044E-5	3.863463649250044E-5
10	3.863463649250044E-5	0.0	0.0	1.1590390947750132E-4	7.726927298500088E-5
11	7.726927298500088E-5	0.0	0.0	1.1590390947750132E-4	7.726927298500088E-5
12	7.726927298500088E-5	0.0	0.0	3.090770919400035E-4	7.726927298500088E-5
13	7.726927298500088E-5	0.0	0.0	3.090770919400035E-4	7.726927298500088E-5
14	7.726927298500088E-5	0.0	0.0	4.249810014175048E-4	7.726927298500088E-5
15	1.1590390947750132E-4	0.0	0.0	4.6361563791000527E-4	7.726927298500088E-5
16	1.1590390947750132E-4	0.0	0.0	8.113273663425092E-4	7.726927298500088E-5
17	1.1590390947750132E-4	0.0	0.0	0.0012749430042525145	7.726927298500088E-5
18	1.1590390947750132E-4	0.0	0.0	0.0014681161867150167	7.726927298500088E-5
19	1.1590390947750132E-4	0.0	0.0	0.002549886008505029	7.726927298500088E-5
20	1.1590390947750132E-4	0.0	0.0	0.00440434856014505	7.726927298500088E-5
21	1.1590390947750132E-4	0.0	0.0	0.0088473317567826	7.726927298500088E-5
22	1.1590390947750132E-4	0.0	0.0	0.015569758506477677	7.726927298500088E-5
23	1.1590390947750132E-4	0.0	0.0	0.018969606517817716	7.726927298500088E-5
24	1.1590390947750132E-4	0.0	0.0	0.02472616735520028	7.726927298500088E-5
25	1.1590390947750132E-4	0.0	0.0	0.02913051591534533	1.1590390947750132E-4
26	1.1590390947750132E-4	0.0	0.0	0.03708925103280042	1.1590390947750132E-4
27	1.1590390947750132E-4	0.0	0.0	0.08209860254656343	1.1590390947750132E-4
28	1.1590390947750132E-4	0.0	0.0	0.17416494130819196	1.1590390947750132E-4
29	1.1590390947750132E-4	0.0	0.0	0.29470500716479336	1.1590390947750132E-4
30	1.1590390947750132E-4	0.0	0.0	0.4576659038901602	1.1590390947750132E-4
31	1.1590390947750132E-4	0.0	0.0	0.6490232584375148	1.1590390947750132E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9055	0.0	21.309221	1
ACCGTTA	400	0.0	19.8875	8
CCGTTAT	435	0.0	18.712643	9
GGACCGT	505	0.0	17.217823	6
TGTTACG	260	0.0	17.076923	16
GTATTAG	700	0.0	16.914286	1
GTATTGG	1120	0.0	16.848215	1
CCGTTTA	415	0.0	16.493977	27
ATTAGAC	215	0.0	16.348837	3
TATACCG	125	1.6599552E-7	16.28	5
GACCGTT	480	0.0	16.1875	7
CGTTATT	280	0.0	15.857143	10
ATTAGAG	670	0.0	15.738806	3
TTCGCTA	345	0.0	15.550725	26
CGCCGTT	465	0.0	15.516129	25
TAATACT	700	0.0	15.328572	4
TATGTCG	85	5.3673727E-4	15.235294	5
CGTTTAC	440	0.0	15.136364	28
TCGCTAC	370	0.0	15.0	27
ATTGGAC	1385	0.0	14.693141	3
>>END_MODULE