FastQCFastQC Report
Wed 25 May 2016
SRR2088443_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088443_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2608939
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT269281.0321437181934878No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT228580.8761416039240473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT189920.7279587602469816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97170.3724502565985636No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG67690.25945413058718503No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA62070.23791280670034828No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG55800.2138800485561372No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54510.20893550979919426No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA49160.18842908937311298No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG45650.17497534438329143No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA36020.1380637876163452No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29870.1144909865658032No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG26330.10092225230256438No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26250.1006156142401183No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78500.022.27071
GTATTGG11100.018.6666661
TCTAATA8900.016.6292152
CTAATAC9650.016.4870453
CGAGTTC3500.016.38571414
GTATTAG9100.016.0604381
TACTGGT9000.016.0333337
ATTGGAC14500.015.820693
CGAACTA7200.015.67361124
TAATACT9950.015.6180914
GGGTTAG3800.015.5789487
ATTAGAG7600.015.3355263
CTCTAAT8650.015.1849711
ATGCTAG8500.015.01764815
ATACTGG9650.014.9533676
GGGGTTA4600.014.8804356
TGGTCGG1001.094209E-414.810
TTAACGG6300.014.68253935
TTGGACC17750.014.591554
AATACTG10700.014.5233655
Produced by FastQC (version 0.11.5)