##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088443_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2608939
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33220707728314	31.0	31.0	34.0	30.0	34.0
2	31.558979339877244	31.0	31.0	34.0	30.0	34.0
3	31.669668014468716	31.0	31.0	34.0	30.0	34.0
4	35.3768359474867	37.0	35.0	37.0	33.0	37.0
5	35.24285274588635	37.0	35.0	37.0	33.0	37.0
6	35.29810240868031	37.0	35.0	37.0	32.0	37.0
7	35.14633113307747	37.0	35.0	37.0	32.0	37.0
8	35.179980444157565	37.0	35.0	37.0	32.0	37.0
9	36.716817066248005	39.0	35.0	39.0	32.0	39.0
10	36.55573127620079	38.0	35.0	39.0	32.0	39.0
11	36.59412657789239	38.0	35.0	39.0	32.0	39.0
12	36.446944524191636	38.0	35.0	39.0	32.0	39.0
13	36.465975632239775	38.0	35.0	39.0	32.0	39.0
14	37.42586737367183	39.0	36.0	41.0	32.0	41.0
15	37.44340745414132	39.0	36.0	41.0	32.0	41.0
16	37.30488064305068	39.0	36.0	41.0	32.0	41.0
17	37.37096957805453	39.0	36.0	41.0	32.0	41.0
18	37.24463661281464	39.0	36.0	40.0	31.0	41.0
19	37.32938370732317	39.0	36.0	40.0	31.0	41.0
20	37.232670445725255	39.0	36.0	40.0	31.0	41.0
21	37.071418687826736	39.0	36.0	40.0	31.0	41.0
22	36.984714475884644	39.0	36.0	40.0	31.0	41.0
23	36.80324070436296	39.0	35.0	40.0	30.0	41.0
24	36.996690225413474	39.0	36.0	40.0	31.0	41.0
25	36.964209205351295	39.0	36.0	40.0	31.0	41.0
26	36.791255755692255	39.0	36.0	40.0	30.0	41.0
27	36.728051134963295	39.0	35.0	40.0	30.0	41.0
28	36.545763622683396	39.0	35.0	40.0	30.0	41.0
29	36.37705059413041	39.0	35.0	40.0	30.0	41.0
30	36.20160839329704	38.0	35.0	40.0	30.0	41.0
31	35.96281783514294	38.0	35.0	40.0	29.0	41.0
32	35.81290555279369	38.0	34.0	40.0	28.0	41.0
33	35.62684447585781	38.0	34.0	40.0	27.0	41.0
34	35.51128830532259	38.0	34.0	40.0	27.0	41.0
35	35.28066658515205	38.0	34.0	40.0	26.0	41.0
36	35.140648363185186	38.0	34.0	40.0	25.0	41.0
37	34.97805391387073	38.0	33.0	40.0	25.0	41.0
38	34.92046690244578	38.0	33.0	40.0	25.0	41.0
39	34.8444513267654	38.0	33.0	40.0	24.0	41.0
40	34.72974646015104	38.0	33.0	40.0	24.0	41.0
41	34.53810840345443	38.0	33.0	40.0	23.0	41.0
42	34.32893103288348	38.0	33.0	40.0	22.0	41.0
43	34.002555828250486	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	3.0
13	5.0
14	10.0
15	18.0
16	37.0
17	87.0
18	237.0
19	494.0
20	1155.0
21	2316.0
22	4338.0
23	7472.0
24	12087.0
25	17949.0
26	26311.0
27	36482.0
28	49098.0
29	63951.0
30	80596.0
31	99235.0
32	121242.0
33	147641.0
34	179211.0
35	216504.0
36	273316.0
37	358414.0
38	465596.0
39	445133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.261945948142134	21.524918750495893	13.4608359950156	23.752299306346373
2	19.0399238924329	23.38372035528619	34.97088279948286	22.605472952798053
3	19.481252723808414	25.133665447908133	32.271164638192005	23.113917190091453
4	13.839419012863083	16.424032911463243	36.74723709523297	32.989310980440706
5	12.479632524945965	38.72512925752576	35.319415287210624	13.47582293031765
6	32.34717254792082	37.79984890409473	13.831906380333153	16.021072167651294
7	26.509665423377086	32.52996716289649	21.795335191815525	19.1650322219109
8	26.84018292493615	34.61867065500574	19.09404551045463	19.44710090960348
9	24.76493317781673	14.403594718006055	21.488390491307	39.343081612870215
10	16.320772543934524	27.427049846700136	33.863459436958856	22.38871817240648
11	33.516115171723065	22.472851990790126	23.393801081589103	20.617231755897706
12	21.121651368621496	27.17438008324457	30.720304307613173	20.983664240520763
13	30.905168729510347	21.066839814959263	26.108889475760073	21.91910197977032
14	20.68331225835483	22.54564020086326	27.505932488264385	29.265115052517515
15	22.964354475133376	29.41973729550595	25.25747056562074	22.358437663739934
16	22.285381145362155	28.181034512497227	26.183325865418855	23.350258476721763
17	22.035509454226414	26.77601891036931	26.87697949242968	24.311492142974597
18	22.8879632678265	25.981596350087145	29.12436818185477	22.00607220023159
19	23.00935361079734	24.881417311788432	29.953440843193345	22.155788234220886
20	23.645244292794885	24.872639797250915	29.91495776635636	21.56715814359784
21	22.683972296784248	25.584461729461673	30.189245513214374	21.542320460539706
22	21.707828354744972	26.132692255357448	29.673863589758138	22.485615800139445
23	22.58500486212978	26.173743425967416	30.562654013758085	20.678597698144724
24	22.581516854169454	26.351785150975164	28.13634201489571	22.930355979959668
25	22.43513550910926	26.27543227342609	29.397927663314473	21.891504554150174
26	22.289022472353704	26.948234512190588	29.347485702042093	21.415257313413612
27	21.4471093421502	26.9656362222344	28.860659448151143	22.726594987464253
28	21.747691302862965	26.595217442799544	29.296698772949465	22.36039248138803
29	22.37871410561918	26.54393222685544	28.656745136624508	22.420608530900875
30	21.999057854553133	26.6607996584052	30.10967293600962	21.230469551032048
31	22.514056480431314	26.44304830431068	29.924731854596832	21.118163360661175
32	21.914770717138268	26.286739551978794	29.11551400780164	22.6829757230813
33	21.80070135790833	26.338714703563404	29.72679698528789	22.133786953240378
34	21.479344668464844	26.751334546342402	29.96666460963633	21.80265617555642
35	21.005090575134183	28.565980270140468	28.945214893870652	21.4837142608547
36	23.0170962218741	26.50947377458806	28.87334659798485	21.600083405552986
37	21.862565587006824	27.069279887341175	28.833636968898084	22.234517556753914
38	21.802502856525198	25.826897447583097	30.068123478548177	22.302476217343525
39	21.292065471825904	25.840351192572918	30.604778417586616	22.262804918014563
40	20.352948075826994	26.624309728974115	30.36023456278587	22.662507632413025
41	21.679924291062385	25.341566054246574	30.48388636146725	22.494623293223796
42	20.397640573428507	27.169550533761043	29.9045320722332	22.528276820577254
43	20.918235343946332	26.618330286756418	29.298691920355363	23.164742448941887
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	133.0
1	275.5
2	418.0
3	1048.5
4	1679.0
5	1679.0
6	2671.0
7	3663.0
8	4036.5
9	4410.0
10	6651.5
11	8893.0
12	8893.0
13	16770.5
14	24648.0
15	39554.5
16	54461.0
17	50334.0
18	46207.0
19	46207.0
20	49152.5
21	52098.0
22	42250.5
23	32403.0
24	36982.5
25	41562.0
26	41562.0
27	46887.0
28	52212.0
29	58447.0
30	64682.0
31	73682.0
32	82682.0
33	82682.0
34	93630.0
35	104578.0
36	116049.0
37	127520.0
38	135291.5
39	143063.0
40	143063.0
41	146768.0
42	150473.0
43	159979.5
44	169486.0
45	172962.5
46	176439.0
47	176439.0
48	215052.5
49	253666.0
50	241695.0
51	229724.0
52	212644.0
53	195564.0
54	195564.0
55	175780.5
56	155997.0
57	133565.0
58	111133.0
59	99590.5
60	88048.0
61	88048.0
62	78673.5
63	69299.0
64	60684.0
65	52069.0
66	45062.0
67	38055.0
68	38055.0
69	32801.0
70	27547.0
71	22902.5
72	18258.0
73	15097.0
74	11936.0
75	11936.0
76	9515.0
77	7094.0
78	5528.0
79	3962.0
80	3124.0
81	2286.0
82	2286.0
83	1775.5
84	1265.0
85	941.0
86	617.0
87	476.0
88	335.0
89	335.0
90	286.5
91	238.0
92	158.5
93	79.0
94	59.0
95	39.0
96	39.0
97	26.0
98	13.0
99	9.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2608939.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.59077583449644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69537102195491	40.017460552312855
2	11.123235724250202	11.03219778711109
3	3.4460221829734268	5.126727407896118
4	1.4293402159318636	2.835283609580312
5	0.7533672110128415	1.8680032241198803
6	0.46561345850118757	1.3854079587634203
7	0.3225297923820743	1.1196151843776287
8	0.24196368805568302	0.9599333611565921
9	0.1716644817316724	0.7661677349070337
>10	1.122984237994748	10.893027079188638
>50	0.1267467861680674	4.358146515450526
>100	0.08672958889173728	8.571735709436068
>500	0.008612412509920566	2.9152972218346584
>1k	0.005198483226708302	4.20701318042069
>5k	3.879465094558434E-4	1.2976029605124233
>10k+	2.3276790567350607E-4	2.646380512932139
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	26928	1.0321437181934878	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	22858	0.8761416039240473	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18992	0.7279587602469816	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9717	0.3724502565985636	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6769	0.25945413058718503	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6207	0.23791280670034828	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5580	0.2138800485561372	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5451	0.20893550979919426	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4916	0.18842908937311298	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4565	0.17497534438329143	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3602	0.1380637876163452	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2987	0.1144909865658032	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	2633	0.10092225230256438	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2625	0.1006156142401183	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.832975780575935E-5	0.0
5	0.0	0.0	0.0	3.832975780575935E-5	0.0
6	3.832975780575935E-5	0.0	0.0	3.832975780575935E-5	0.0
7	3.832975780575935E-5	0.0	0.0	3.832975780575935E-5	0.0
8	3.832975780575935E-5	0.0	0.0	3.832975780575935E-5	0.0
9	3.832975780575935E-5	0.0	0.0	1.9164878902879677E-4	0.0
10	3.832975780575935E-5	0.0	0.0	1.9164878902879677E-4	0.0
11	3.832975780575935E-5	0.0	0.0	2.6830830464031545E-4	0.0
12	3.832975780575935E-5	0.0	0.0	4.599570936691122E-4	0.0
13	3.832975780575935E-5	0.0	0.0	5.366166092806309E-4	0.0
14	3.832975780575935E-5	0.0	0.0	5.749463670863903E-4	0.0
15	3.832975780575935E-5	0.0	0.0	9.199141873382244E-4	0.0
16	3.832975780575935E-5	0.0	0.0	0.0014948605544246147	0.0
17	3.832975780575935E-5	0.0	0.0	0.001993147405899486	0.0
18	3.832975780575935E-5	0.0	0.0	0.00237644498395708	3.832975780575935E-5
19	3.832975780575935E-5	0.0	0.0	0.00295139135104347	3.832975780575935E-5
20	3.832975780575935E-5	0.0	0.0	0.004906208999137197	3.832975780575935E-5
21	3.832975780575935E-5	0.0	0.0	0.008470876475072817	3.832975780575935E-5
22	3.832975780575935E-5	0.0	0.0	0.014373659177159758	3.832975780575935E-5
23	3.832975780575935E-5	0.0	0.0	0.01835995398895873	3.832975780575935E-5
24	3.832975780575935E-5	0.0	0.0	0.024991002089355098	3.832975780575935E-5
25	3.832975780575935E-5	0.0	0.0	0.029743892057269256	3.832975780575935E-5
26	3.832975780575935E-5	0.0	0.0	0.0366815782201117	3.832975780575935E-5
27	3.832975780575935E-5	0.0	0.0	0.07669784536932446	3.832975780575935E-5
28	3.832975780575935E-5	0.0	0.0	0.1620965457605563	3.832975780575935E-5
29	3.832975780575935E-5	0.0	0.0	0.2700714734993804	3.832975780575935E-5
30	3.832975780575935E-5	0.0	0.0	0.41572455316126594	3.832975780575935E-5
31	7.66595156115187E-5	0.0	0.0	0.6036936854407098	7.66595156115187E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7850	0.0	22.2707	1
GTATTGG	1110	0.0	18.666666	1
TCTAATA	890	0.0	16.629215	2
CTAATAC	965	0.0	16.487045	3
CGAGTTC	350	0.0	16.385714	14
GTATTAG	910	0.0	16.060438	1
TACTGGT	900	0.0	16.033333	7
ATTGGAC	1450	0.0	15.82069	3
CGAACTA	720	0.0	15.673611	24
TAATACT	995	0.0	15.618091	4
GGGTTAG	380	0.0	15.578948	7
ATTAGAG	760	0.0	15.335526	3
CTCTAAT	865	0.0	15.184971	1
ATGCTAG	850	0.0	15.017648	15
ATACTGG	965	0.0	14.953367	6
GGGGTTA	460	0.0	14.880435	6
TGGTCGG	100	1.094209E-4	14.8	10
TTAACGG	630	0.0	14.682539	35
TTGGACC	1775	0.0	14.59155	4
AATACTG	1070	0.0	14.523365	5
>>END_MODULE