FastQCFastQC Report
Wed 25 May 2016
SRR2088442_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088442_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2360453
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT406001.7200088288137911No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT336751.4266329386774488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT283591.2014219304514855No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139470.5908611609720676No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG102790.43546725988613205No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA96660.4094976684560125No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG88350.3742925616396514No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA83330.35302545740160896No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA77120.32671694797566403No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG71310.30210302852884596No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA55120.23351449912368516No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43240.1831851767436166No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42800.181321127766577No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA42790.18127876301709883No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA41770.17695755857032527No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG38650.16373975673313554No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36260.15361458160785238No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35460.1502254016495986No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35060.14853081167047175No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33550.14213373449926772No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32150.1362026695723236No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31220.1322627478708536No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC30530.12933958015685973No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29760.12607749444704044No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT27870.11807055679566592No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA27490.11646069631549537No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT25160.10658970968708124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGT802.7288479E-823.1254
GGTATCA117150.021.492531
GTATTGG13500.018.51
ATTGGAC15600.017.5512833
GTATTAG4550.017.4835171
ACCGTTA4350.017.0114948
CGTTCGC3750.016.77333523
TAGATGT5200.016.3653854
ACGTTTA1251.6597733E-716.27999926
TATACTG2400.016.18755
TTGGACC20400.015.9607844
TATTGGA16100.015.8571432
ACGTTCG3050.015.77049322
CAGTTCG3650.015.2054813
AATACCT6000.015.10833434
TCTAACG1353.977002E-715.0740752
TATACAG2109.094947E-1214.976195
TGGACCC21600.014.6458345
GTCGGGT3700.014.50000124
TTCGTTA3450.014.47826130
Produced by FastQC (version 0.11.5)