##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088442_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2360453
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.299236629579152	31.0	31.0	33.0	30.0	34.0
2	31.506483289436392	31.0	31.0	34.0	30.0	34.0
3	31.60097405032	31.0	31.0	34.0	30.0	34.0
4	35.30178275102279	37.0	35.0	37.0	33.0	37.0
5	35.19406402076211	37.0	35.0	37.0	33.0	37.0
6	35.26333843546133	37.0	35.0	37.0	32.0	37.0
7	35.08343906868724	37.0	35.0	37.0	32.0	37.0
8	35.15336632417591	37.0	35.0	37.0	32.0	37.0
9	36.65217693383431	39.0	35.0	39.0	32.0	39.0
10	36.52747332821285	38.0	35.0	39.0	32.0	39.0
11	36.553304810559666	38.0	35.0	39.0	32.0	39.0
12	36.40253036175683	38.0	35.0	39.0	32.0	39.0
13	36.43597055310993	38.0	35.0	39.0	32.0	39.0
14	37.30653438132426	39.0	36.0	41.0	32.0	41.0
15	37.33658708730909	39.0	36.0	41.0	32.0	41.0
16	37.17511342102554	39.0	36.0	40.0	31.0	41.0
17	37.32060159638849	39.0	36.0	40.0	32.0	41.0
18	37.19763282725816	39.0	36.0	40.0	31.0	41.0
19	37.30178148008031	39.0	36.0	40.0	31.0	41.0
20	37.225659650922935	39.0	36.0	40.0	31.0	41.0
21	37.069332454405995	39.0	36.0	40.0	31.0	41.0
22	36.988687340946846	39.0	36.0	40.0	31.0	41.0
23	36.78799832066133	39.0	35.0	40.0	30.0	41.0
24	36.9637264542018	39.0	36.0	40.0	31.0	41.0
25	36.934479525752046	39.0	36.0	40.0	31.0	41.0
26	36.72939728094565	39.0	35.0	40.0	30.0	41.0
27	36.660982023365854	39.0	35.0	40.0	30.0	41.0
28	36.44829149320067	38.0	35.0	40.0	30.0	41.0
29	36.25004522437007	38.0	35.0	40.0	30.0	41.0
30	36.04171148504122	38.0	35.0	40.0	29.0	41.0
31	35.72122554441881	38.0	34.0	40.0	28.0	41.0
32	35.56473693820635	38.0	34.0	40.0	27.0	41.0
33	35.32646953783871	38.0	34.0	40.0	27.0	41.0
34	35.19257066334301	38.0	34.0	40.0	26.0	41.0
35	34.905762156670775	38.0	33.0	40.0	25.0	41.0
36	34.7220702975234	38.0	33.0	40.0	24.0	41.0
37	34.52899295177663	37.0	33.0	40.0	24.0	41.0
38	34.39150239382017	37.0	33.0	40.0	23.0	41.0
39	34.259169744112675	37.0	33.0	40.0	23.0	41.0
40	34.07025049852719	37.0	33.0	40.0	21.0	41.0
41	33.80169950429006	37.0	33.0	40.0	20.0	41.0
42	33.503239420568846	37.0	32.0	40.0	18.0	41.0
43	33.14156392861879	37.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	6.0
14	6.0
15	11.0
16	33.0
17	109.0
18	229.0
19	566.0
20	1198.0
21	2433.0
22	4407.0
23	7470.0
24	12077.0
25	18150.0
26	26476.0
27	36593.0
28	49092.0
29	63378.0
30	78516.0
31	94380.0
32	114619.0
33	138559.0
34	167939.0
35	199535.0
36	250278.0
37	332485.0
38	394591.0
39	367314.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.109417133067254	21.3376415459236	14.384145755073286	22.168795565935863
2	19.28057877026147	24.214843506733665	34.65398379039956	21.850593932605307
3	20.316312165503824	24.73520972457405	33.28708514848633	21.66139296143579
4	13.761849949988413	16.01323983150692	35.72238040748958	34.50252981101509
5	12.320897725987342	39.073474455962476	34.37666414031544	14.22896367773474
6	32.43051227878716	36.71532540575898	12.663840373013146	18.19032194244071
7	25.7414572541796	33.556821508413854	20.598842679773753	20.102878557632796
8	28.16179775661706	33.554237258695686	18.173206583651528	20.11075840103573
9	24.20802278206768	14.382747718340505	22.103299663242606	39.30592983634921
10	17.156494960924874	27.32937279412045	32.26681488680351	23.247317358151168
11	32.68287909142863	22.228868780695908	23.757558400866273	21.33069372700918
12	21.80767844138392	27.48002184326483	30.470846062175355	20.241453653175896
13	32.907835911157726	20.433577792059403	26.477544776362844	20.18104152042002
14	20.551860172602463	23.278158895771277	27.935358170656226	28.234622760970034
15	22.464670976291416	29.4424841333422	26.14129575975459	21.951549130611795
16	21.473505297500097	28.172854956230857	26.94902207330542	23.40461767296362
17	21.924350961446805	26.009753212624865	26.813243051227882	25.25265277470045
18	23.59746201258826	24.376931038237153	30.057747390013695	21.96785955916089
19	22.765757250832785	23.7218025523067	31.616473617564083	21.89596657929643
20	24.199380373174133	23.21257826357907	31.415325787041727	21.172715576205075
21	22.715639752200108	24.30516515262113	32.35116310301455	20.628031992164217
22	20.875103211120916	24.896111043092155	31.738907743556005	22.489878002230927
23	22.34100827256463	25.235156133165965	32.83293503408032	19.59090056018908
24	22.083600054735257	25.139115246098946	29.728192003822997	23.0490926953428
25	21.911810995601268	25.669437180066705	31.024256784608717	21.394495039723306
26	21.842120982709677	26.42581741724999	31.02154544064211	20.710516159398217
27	20.251960111046483	26.65700185515238	30.67534070790649	22.415697325894648
28	21.0395208038457	26.129391265151224	30.52964833445106	22.301439596552015
29	21.370389497270228	26.002508840464095	29.858548337967335	22.768553324298342
30	21.234695204691644	26.409168070705075	32.11807225138564	20.23806447321764
31	22.279537021071803	25.92366804168522	31.802115949777438	19.99467898746554
32	21.889357678377834	25.71963940819834	30.191069256621507	22.19993365680232
33	21.25371697720734	25.9565007225308	30.965623971330924	21.824158328930928
34	20.257848811223948	26.962409334140524	31.612194777866794	21.167547076768738
35	19.688127660241488	29.756491656474417	30.13442758656919	20.42095309671491
36	22.612523951970235	26.372522562406452	30.165353853688252	20.849599631935057
37	21.378820082416382	27.654437516866466	29.850414306067524	21.116328094649628
38	21.134799125422113	25.660752406423683	31.50929080138431	21.695157666769894
39	20.673913015849077	25.403810200838567	32.44241677339053	21.479860009921826
40	19.20360202045963	26.500167552584188	31.694509486102877	22.6017209408533
41	21.287058035046662	25.058368033593553	31.723825892741775	21.930748038618013
42	19.526379046733826	27.59313572437155	31.085177294358328	21.795307934536297
43	19.91316920946954	27.590890392649207	29.904175173155323	22.591765224725933
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	118.0
1	292.5
2	467.0
3	1250.0
4	2033.0
5	2033.0
6	3374.5
7	4716.0
8	5363.0
9	6010.0
10	8985.0
11	11960.0
12	11960.0
13	23351.5
14	34743.0
15	56897.0
16	79051.0
17	71150.5
18	63250.0
19	63250.0
20	65487.0
21	67724.0
22	50331.5
23	32939.0
24	35972.5
25	39006.0
26	39006.0
27	40874.5
28	42743.0
29	45816.5
30	48890.0
31	52751.5
32	56613.0
33	56613.0
34	62074.5
35	67536.0
36	75205.5
37	82875.0
38	88732.0
39	94589.0
40	94589.0
41	98861.0
42	103133.0
43	113681.0
44	124229.0
45	139574.0
46	154919.0
47	154919.0
48	219860.5
49	284802.0
50	266338.5
51	247875.0
52	225637.5
53	203400.0
54	203400.0
55	176711.0
56	150022.0
57	123913.0
58	97804.0
59	85282.0
60	72760.0
61	72760.0
62	64214.5
63	55669.0
64	48279.5
65	40890.0
66	35350.5
67	29811.0
68	29811.0
69	25854.5
70	21898.0
71	18417.5
72	14937.0
73	12341.0
74	9745.0
75	9745.0
76	7953.5
77	6162.0
78	4805.0
79	3448.0
80	2618.0
81	1788.0
82	1788.0
83	1373.0
84	958.0
85	716.5
86	475.0
87	365.0
88	255.0
89	255.0
90	190.0
91	125.0
92	92.5
93	60.0
94	38.0
95	16.0
96	16.0
97	11.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2360453.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.265020176431804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.57853007899823	34.42966709478643
2	11.16970328219993	9.66514875738273
3	3.6303592363361448	4.712026968233365
4	1.5701236482189271	2.7172572527873835
5	0.8792730221430077	1.9020882521804696
6	0.5594149232611708	1.452185876513496
7	0.37242354347426737	1.1279038485814683
8	0.2832612693040975	0.9804243625314769
9	0.2128431131096706	0.828779542479401
>10	1.455004316262418	12.406873781508567
>50	0.15777984040123555	4.763534095794928
>100	0.11623990577885089	9.754286038256174
>500	0.007571073068212633	2.2865573131978465
>1k	0.006391165577062612	5.569117721724088
>5k	5.899537455750104E-4	2.007448428883594
>10k+	4.916281213125087E-4	5.396700665158676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40600	1.7200088288137911	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33675	1.4266329386774488	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28359	1.2014219304514855	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13947	0.5908611609720676	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10279	0.43546725988613205	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9666	0.4094976684560125	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8835	0.3742925616396514	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8333	0.35302545740160896	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7712	0.32671694797566403	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7131	0.30210302852884596	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5512	0.23351449912368516	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4324	0.1831851767436166	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4280	0.181321127766577	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4279	0.18127876301709883	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4177	0.17695755857032527	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3865	0.16373975673313554	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3626	0.15361458160785238	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3546	0.1502254016495986	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3506	0.14853081167047175	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3355	0.14213373449926772	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3215	0.1362026695723236	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3122	0.1322627478708536	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3053	0.12933958015685973	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2976	0.12607749444704044	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2787	0.11807055679566592	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2749	0.11646069631549537	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2516	0.10658970968708124	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	4.23647494781722E-5
4	0.0	0.0	0.0	0.0	4.23647494781722E-5
5	0.0	0.0	0.0	0.0	4.23647494781722E-5
6	0.0	0.0	0.0	0.0	4.23647494781722E-5
7	0.0	0.0	0.0	0.0	4.23647494781722E-5
8	0.0	0.0	0.0	0.0	4.23647494781722E-5
9	0.0	0.0	0.0	4.23647494781722E-5	4.23647494781722E-5
10	4.23647494781722E-5	0.0	0.0	1.270942484345166E-4	4.23647494781722E-5
11	8.47294989563444E-5	0.0	0.0	4.23647494781722E-4	8.47294989563444E-5
12	8.47294989563444E-5	0.0	0.0	6.35471242172583E-4	8.47294989563444E-5
13	1.694589979126888E-4	0.0	0.0	8.049302400852718E-4	8.47294989563444E-5
14	2.541884968690332E-4	0.0	0.0	0.0010167539874761328	8.47294989563444E-5
15	2.541884968690332E-4	0.0	0.0	0.0013980367327796825	8.47294989563444E-5
16	2.541884968690332E-4	0.0	0.0	0.0018640489770395767	8.47294989563444E-5
17	2.541884968690332E-4	0.0	0.0	0.002541884968690332	8.47294989563444E-5
18	2.965532463472054E-4	0.0	0.0	0.003219720960341087	8.47294989563444E-5
19	2.965532463472054E-4	0.0	0.0	0.004024651200426358	8.47294989563444E-5
20	2.965532463472054E-4	0.0	0.0	0.007074913162854757	8.47294989563444E-5
21	2.965532463472054E-4	0.0	0.0	0.013514355083536931	8.47294989563444E-5
22	2.965532463472054E-4	4.23647494781722E-5	0.0	0.02135183373699879	8.47294989563444E-5
23	2.965532463472054E-4	4.23647494781722E-5	0.0	0.026901615918639345	8.47294989563444E-5
24	2.965532463472054E-4	4.23647494781722E-5	0.0	0.03584057805853368	8.47294989563444E-5
25	2.965532463472054E-4	4.23647494781722E-5	0.0	0.041856372484434134	8.47294989563444E-5
26	2.965532463472054E-4	4.23647494781722E-5	0.0	0.05337958434249697	8.47294989563444E-5
27	2.965532463472054E-4	4.23647494781722E-5	0.0	0.11192766812133095	8.47294989563444E-5
28	2.965532463472054E-4	4.23647494781722E-5	0.0	0.2287272824326517	8.47294989563444E-5
29	3.389179958253776E-4	4.23647494781722E-5	0.0	0.3844601015144127	8.47294989563444E-5
30	3.389179958253776E-4	4.23647494781722E-5	0.0	0.5984444511286605	8.47294989563444E-5
31	3.8128274530354976E-4	4.23647494781722E-5	0.0	0.8234860003567112	8.47294989563444E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGT	80	2.7288479E-8	23.125	4
GGTATCA	11715	0.0	21.49253	1
GTATTGG	1350	0.0	18.5	1
ATTGGAC	1560	0.0	17.551283	3
GTATTAG	455	0.0	17.483517	1
ACCGTTA	435	0.0	17.011494	8
CGTTCGC	375	0.0	16.773335	23
TAGATGT	520	0.0	16.365385	4
ACGTTTA	125	1.6597733E-7	16.279999	26
TATACTG	240	0.0	16.1875	5
TTGGACC	2040	0.0	15.960784	4
TATTGGA	1610	0.0	15.857143	2
ACGTTCG	305	0.0	15.770493	22
CAGTTCG	365	0.0	15.20548	13
AATACCT	600	0.0	15.108334	34
TCTAACG	135	3.977002E-7	15.074075	2
TATACAG	210	9.094947E-12	14.97619	5
TGGACCC	2160	0.0	14.645834	5
GTCGGGT	370	0.0	14.500001	24
TTCGTTA	345	0.0	14.478261	30
>>END_MODULE