FastQCFastQC Report
Wed 25 May 2016
SRR2088441_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088441_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002315
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143791.434578949731372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116981.1670981677416778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86740.8653966068551304No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48870.48757127250415283No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG30530.3045948628924041No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29590.29521657363204185No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG28760.28693574375321135No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26550.26488678708789154No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA25030.2497218938158164No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG20260.20213206427121214No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT16090.1605283768076902No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14290.1425699505644433No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12150.12121937714191647No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA11660.11633069444236593No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC11370.11343739243650948No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA11080.11054409043065304No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11000.10974593815317539No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC10990.1096461691184907No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10680.10655332904326484No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA10540.10515656255767898No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG10190.1016646463437143No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTCT358.868601E-426.4285713
GGTATCA39050.022.8348271
CTAGTGC450.003825472220.5555553
TTACGCA450.003825472220.55555510
TAGACTA852.7225522E-517.4117645
TAGCTCG650.00157974317.07692327
TAGACAG1251.656972E-716.2799995
GTATTGG4450.016.2134821
GTATAAT1403.4709956E-815.8571421
TCCTATA957.058233E-515.5789482
TTCGTTA855.3633E-415.23529430
TATTAAG855.3633E-415.2352942
ATACCGT855.3633E-415.2352946
CATACCC1101.4514208E-515.1363643
GTATCAA58950.015.0949962
ACCGTTA1353.9703627E-715.0740738
TTGGACC6550.014.9694664
TCTATCG750.00410391814.829
TCTATAC750.00410391814.83
CCTTAAC1252.9576458E-614.7999992
Produced by FastQC (version 0.11.5)