##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088441_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1002315
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98281478377556	31.0	31.0	33.0	30.0	34.0
2	31.208374612771433	31.0	31.0	34.0	28.0	34.0
3	31.281504317504975	31.0	31.0	34.0	28.0	34.0
4	35.03113392496371	35.0	35.0	37.0	32.0	37.0
5	34.8724642452722	35.0	35.0	37.0	32.0	37.0
6	34.97650040157036	35.0	35.0	37.0	32.0	37.0
7	34.806811231997926	35.0	35.0	37.0	32.0	37.0
8	34.87165910916229	35.0	35.0	37.0	32.0	37.0
9	36.32926774516993	38.0	35.0	39.0	32.0	39.0
10	36.134525573297815	38.0	35.0	39.0	32.0	39.0
11	36.1993824296753	38.0	35.0	39.0	32.0	39.0
12	36.01142654754244	37.0	35.0	39.0	30.0	39.0
13	36.06434404353921	38.0	35.0	39.0	31.0	39.0
14	36.883258257134734	38.0	36.0	40.0	31.0	41.0
15	36.92568005068267	38.0	36.0	40.0	31.0	41.0
16	36.75553294124103	38.0	36.0	40.0	31.0	41.0
17	36.87287230062405	38.0	36.0	40.0	31.0	41.0
18	36.727687403660525	38.0	36.0	40.0	31.0	41.0
19	36.82138449489432	39.0	36.0	40.0	31.0	41.0
20	36.73703376682979	39.0	35.0	40.0	30.0	41.0
21	36.5748911270409	38.0	35.0	40.0	30.0	41.0
22	36.47946004998428	38.0	35.0	40.0	30.0	41.0
23	36.267053770521244	38.0	35.0	40.0	30.0	41.0
24	36.45407581448946	38.0	35.0	40.0	30.0	41.0
25	36.408756728174275	38.0	35.0	40.0	30.0	41.0
26	36.18465452477515	38.0	35.0	40.0	30.0	41.0
27	36.10733651596554	38.0	35.0	40.0	29.0	41.0
28	35.8890418680754	38.0	34.0	40.0	28.0	41.0
29	35.69251283279208	38.0	34.0	40.0	27.0	41.0
30	35.49647466115942	38.0	34.0	40.0	27.0	41.0
31	35.18920399275677	38.0	34.0	40.0	26.0	41.0
32	35.03360320857215	38.0	33.0	40.0	26.0	41.0
33	34.80292123733557	37.0	33.0	40.0	25.0	41.0
34	34.67707656774567	37.0	33.0	40.0	25.0	41.0
35	34.39253528082489	37.0	33.0	40.0	24.0	41.0
36	34.24886886856926	37.0	33.0	40.0	24.0	41.0
37	34.05699904720572	37.0	33.0	40.0	23.0	41.0
38	33.9806348303677	37.0	33.0	40.0	23.0	41.0
39	33.86084314811212	37.0	33.0	40.0	22.0	41.0
40	33.703199094097165	37.0	32.0	40.0	21.0	41.0
41	33.460180681721816	37.0	32.0	40.0	19.0	41.0
42	33.198324877907645	37.0	31.0	40.0	18.0	41.0
43	32.855417708005966	36.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	1.0
14	6.0
15	10.0
16	25.0
17	56.0
18	140.0
19	362.0
20	783.0
21	1433.0
22	2515.0
23	4241.0
24	6570.0
25	9723.0
26	13621.0
27	18452.0
28	24010.0
29	30151.0
30	37365.0
31	44793.0
32	53708.0
33	64419.0
34	75711.0
35	90295.0
36	111158.0
37	140557.0
38	167564.0
39	104644.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.78080743079771	23.150207270169556	14.929039274080505	22.139946024952238
2	20.16431960012571	24.771354314761325	33.66227184068881	21.402054244424157
3	20.919870499792978	25.39740500740785	32.50325496475659	21.17946952804258
4	15.177663708514789	17.393733506931454	35.22904476137741	32.199558023176344
5	12.397799095094856	39.72663284496391	34.41732389518265	13.458244164758584
6	32.38393119927368	37.78323181834054	13.155245606421134	16.677591375964642
7	25.650618817437632	33.44238088824372	21.307373430508374	19.59962686381028
8	27.390790320408254	34.789861470695335	18.560532367569078	19.25881584132733
9	24.46925367773604	14.595112314990796	21.6548689783152	39.280765028957966
10	16.784942857285383	28.160707961070123	32.930665509345864	22.12368367229863
11	33.386809535924336	22.708429984585685	23.183230820650195	20.721529658839785
12	21.01036101425201	27.906496460693496	30.666806343315223	20.416336181739272
13	32.05269800412046	20.918972578480815	26.252026558517034	20.77630285888169
14	20.309982390765377	23.211066381327228	27.936427171098906	28.54252405680849
15	22.693165322278926	30.003042955557884	25.453674742970023	21.850116979193167
16	21.575851902844914	28.461511600644506	26.77701121902795	23.18562527748263
17	22.018028264567526	26.90890588288113	27.199732618987042	23.8733332335643
18	22.98608720811322	25.56022807201329	29.57483425869113	21.878850461182363
19	22.827354673929854	24.79330350239196	30.87223078573103	21.50711103794715
20	23.479544853663768	24.677571422157705	30.69653751565127	21.14634620852726
21	22.641285424242877	25.40508722307857	31.26951108184553	20.684116270833023
22	21.18346028942997	25.97167557105301	30.737841896010735	22.107022243506282
23	22.265954315759018	26.182587310376476	31.601741967345593	19.94971640651891
24	22.261863785336942	26.140684315808905	29.104423260152746	22.493028638701407
25	22.14004579398692	26.265495378199468	30.15329512179305	21.441163706020564
26	21.96914143757202	27.13009383277712	30.231114968847116	20.66964976080374
27	21.013354085292548	27.045090615225753	29.804801883639374	22.136753415842325
28	21.38878496281109	26.7297206965874	30.138728842729083	21.742765497872426
29	21.831160862603074	26.450068092366173	29.434758533993804	22.28401251103695
30	21.687792759761155	26.63074981418017	31.016995655058537	20.664461771000134
31	22.254480876770277	26.410060709457607	30.80538553249228	20.530072881279835
32	21.768206601717026	26.21251802078189	29.796221746656492	22.223053630844593
33	21.555099943630495	26.377236697046335	30.289080777998933	21.778582581324237
34	20.9915046666966	26.981338202062226	30.777450202780564	21.24970692846061
35	20.51061791951632	28.986396492120743	29.73685917101909	20.76612641734385
36	22.72299626364965	26.453360470510766	29.7146106762844	21.10903258955518
37	21.50581404049625	27.257897966208226	29.64367489262358	21.592613100671944
38	21.45363483535615	25.624180023246186	31.067977631782423	21.85420750961524
39	20.847039104473144	25.701101948988093	31.696622319330746	21.755236627208014
40	19.92637045240269	26.523498101894116	31.120057067887842	22.430074377815355
41	21.194035807106548	25.38982256077181	31.209450122965336	22.206691509156304
42	20.009478058295045	27.366346906910504	30.6565301327427	21.96764490205175
43	20.319061372921688	27.131989444436126	29.841516888403348	22.70743229423884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	66.0
1	128.5
2	191.0
3	352.5
4	514.0
5	514.0
6	948.0
7	1382.0
8	1633.5
9	1885.0
10	3041.5
11	4198.0
12	4198.0
13	8278.0
14	12358.0
15	20520.5
16	28683.0
17	25415.5
18	22148.0
19	22148.0
20	23820.0
21	25492.0
22	19914.5
23	14337.0
24	15645.5
25	16954.0
26	16954.0
27	18597.0
28	20240.0
29	22251.0
30	24262.0
31	26730.0
32	29198.0
33	29198.0
34	32702.0
35	36206.0
36	39694.5
37	43183.0
38	46161.0
39	49139.0
40	49139.0
41	51462.5
42	53786.0
43	56975.0
44	60164.0
45	64179.5
46	68195.0
47	68195.0
48	87206.5
49	106218.0
50	99984.5
51	93751.0
52	85728.5
53	77706.0
54	77706.0
55	68598.0
56	59490.0
57	50407.0
58	41324.0
59	36236.5
60	31149.0
61	31149.0
62	27627.0
63	24105.0
64	20903.5
65	17702.0
66	15222.0
67	12742.0
68	12742.0
69	10903.5
70	9065.0
71	7794.0
72	6523.0
73	5379.5
74	4236.0
75	4236.0
76	3422.0
77	2608.0
78	2024.0
79	1440.0
80	1143.0
81	846.0
82	846.0
83	625.0
84	404.0
85	303.0
86	202.0
87	153.5
88	105.0
89	105.0
90	84.5
91	64.0
92	46.5
93	29.0
94	23.5
95	18.0
96	18.0
97	10.5
98	3.0
99	3.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1002315.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.7215572839146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.0018558566193	47.36435780353664
2	8.448278223459583	9.415024379579028
3	2.433575268894105	4.068078112512024
4	1.0924164209201768	2.4348457670477037
5	0.6400253329627975	1.7831604126921519
6	0.42262098534366693	1.4129459666526945
7	0.29185094713680976	1.1383672488473935
8	0.23343199913423343	1.0405755609325515
9	0.16979122078796322	0.8514928111898055
>10	1.091508851859562	11.35189262877593
>50	0.10587105330302504	4.113380704831904
>100	0.06031213690469707	6.42212079488681
>500	0.004680941904762912	1.7104387695908214
>1k	0.003240652087912785	3.4071338376312656
>5k	1.8003622710626585E-4	0.8701669142188145
>10k+	3.600724542125317E-4	2.6160182870744784
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14379	1.434578949731372	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11698	1.1670981677416778	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8674	0.8653966068551304	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4887	0.48757127250415283	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3053	0.3045948628924041	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2959	0.29521657363204185	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2876	0.28693574375321135	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2655	0.26488678708789154	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2503	0.2497218938158164	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2026	0.20213206427121214	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1609	0.1605283768076902	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1429	0.1425699505644433	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1215	0.12121937714191647	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1166	0.11633069444236593	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1137	0.11343739243650948	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1108	0.11054409043065304	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1100	0.10974593815317539	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1099	0.1096461691184907	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1068	0.10655332904326484	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1054	0.10515656255767898	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1019	0.1016646463437143	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.9907613873881964E-4	0.0
10	9.976903468470491E-5	0.0	0.0	3.9907613873881964E-4	0.0
11	9.976903468470491E-5	0.0	0.0	4.988451734235245E-4	0.0
12	9.976903468470491E-5	0.0	0.0	7.981522774776393E-4	0.0
13	2.9930710405411473E-4	0.0	0.0	8.979213121623441E-4	0.0
14	2.9930710405411473E-4	0.0	0.0	8.979213121623441E-4	0.0
15	2.9930710405411473E-4	0.0	0.0	0.001197228416216459	0.0
16	2.9930710405411473E-4	0.0	0.0	0.0017958426243246883	0.0
17	2.9930710405411473E-4	0.0	0.0	0.003092840075225852	0.0
18	3.9907613873881964E-4	0.0	0.0	0.003491916213964672	0.0
19	3.9907613873881964E-4	0.0	0.0	0.004489606560811721	0.0
20	3.9907613873881964E-4	0.0	0.0	0.007083601462614049	0.0
21	3.9907613873881964E-4	0.0	0.0	0.014167202925228098	0.0
22	3.9907613873881964E-4	0.0	0.0	0.024044337359013884	0.0
23	3.9907613873881964E-4	0.0	0.0	0.030130248474780883	0.0
24	3.9907613873881964E-4	0.0	0.0	0.040306690012620786	0.0
25	3.9907613873881964E-4	0.0	0.0	0.047889136648658355	0.0
26	3.9907613873881964E-4	0.0	0.0	0.05766650204775944	0.0
27	3.9907613873881964E-4	0.0	0.0	0.09897088240722726	0.0
28	3.9907613873881964E-4	0.0	0.0	0.19435007956580516	0.0
29	3.9907613873881964E-4	0.0	0.0	0.31227707856312636	0.0
30	3.9907613873881964E-4	0.0	0.0	0.4992442495622634	0.0
31	3.9907613873881964E-4	0.0	0.0	0.7463721484762774	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTCT	35	8.868601E-4	26.42857	13
GGTATCA	3905	0.0	22.834827	1
CTAGTGC	45	0.0038254722	20.555555	3
TTACGCA	45	0.0038254722	20.555555	10
TAGACTA	85	2.7225522E-5	17.411764	5
TAGCTCG	65	0.001579743	17.076923	27
TAGACAG	125	1.656972E-7	16.279999	5
GTATTGG	445	0.0	16.213482	1
GTATAAT	140	3.4709956E-8	15.857142	1
TCCTATA	95	7.058233E-5	15.578948	2
TTCGTTA	85	5.3633E-4	15.235294	30
TATTAAG	85	5.3633E-4	15.235294	2
ATACCGT	85	5.3633E-4	15.235294	6
CATACCC	110	1.4514208E-5	15.136364	3
GTATCAA	5895	0.0	15.094996	2
ACCGTTA	135	3.9703627E-7	15.074073	8
TTGGACC	655	0.0	14.969466	4
TCTATCG	75	0.004103918	14.8	29
TCTATAC	75	0.004103918	14.8	3
CCTTAAC	125	2.9576458E-6	14.799999	2
>>END_MODULE