FastQCFastQC Report
Wed 25 May 2016
SRR2088440_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088440_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3915502
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT403081.029446543508342No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT356900.9115050892580313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT287820.7350781585605115No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146390.3738728776029229No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG115130.29403637132607774No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA99600.254373513281311No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG89360.22822105569094334No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82990.21195238822506027No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79860.20395852179362953No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG71400.18235209687033743No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA58800.15017231506968964No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45030.11500441067326744No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43960.11227168317114894No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA43720.11165873494637468No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43060.10997312732824553No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA40320.10297530176207291No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115600.023.6691191
GTATTGG17800.016.4213491
TAATACT9550.015.8848164
GTATTAG9400.015.7446811
GTATCAA174150.015.6583412
TTGGACC25500.014.727454
ATTGGAC21950.014.4965843
TTAACGG5550.014.33333335
TATACTG5400.014.0462975
TGGACCC26400.013.8755
ATAATAC4800.013.8749993
ACCGTTA4700.013.7765968
GGACCCT25700.013.6050596
GGACCGT6300.013.5079376
CTAATAC8650.013.47398953
TCTATAC4000.013.41253
TTCGTTA2551.6370905E-1113.05882530
ATTAGAG8650.013.0462443
TACTGGT9950.013.0150767
CCGTTTA4850.012.96907227
Produced by FastQC (version 0.11.5)