##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088440_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3915502
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352790778806906	31.0	31.0	34.0	30.0	34.0
2	31.572936241636448	31.0	31.0	34.0	30.0	34.0
3	31.686029786218985	31.0	31.0	34.0	30.0	34.0
4	35.39066868054211	37.0	35.0	37.0	33.0	37.0
5	35.256808450104224	37.0	35.0	37.0	33.0	37.0
6	35.314199303180025	37.0	35.0	37.0	33.0	37.0
7	35.15952616037484	37.0	35.0	37.0	32.0	37.0
8	35.19492315417027	37.0	35.0	37.0	32.0	37.0
9	36.738142899684384	39.0	35.0	39.0	32.0	39.0
10	36.583195207153516	39.0	35.0	39.0	32.0	39.0
11	36.62034804221783	39.0	35.0	39.0	32.0	39.0
12	36.47538706403419	38.0	35.0	39.0	32.0	39.0
13	36.49652662672628	38.0	35.0	39.0	32.0	39.0
14	37.4625769569266	39.0	36.0	41.0	32.0	41.0
15	37.48037493021329	39.0	36.0	41.0	32.0	41.0
16	37.348597957554354	39.0	36.0	41.0	32.0	41.0
17	37.41305993458821	39.0	36.0	41.0	32.0	41.0
18	37.27888148186363	39.0	36.0	40.0	32.0	41.0
19	37.36389791143	39.0	36.0	41.0	32.0	41.0
20	37.26757769501842	39.0	36.0	40.0	31.0	41.0
21	37.113354813763344	39.0	36.0	40.0	31.0	41.0
22	37.025080819777386	39.0	36.0	40.0	31.0	41.0
23	36.83824347427227	39.0	36.0	40.0	30.0	41.0
24	37.030999601072864	39.0	36.0	40.0	31.0	41.0
25	37.011785206596755	39.0	36.0	40.0	31.0	41.0
26	36.83324845703054	39.0	36.0	40.0	30.0	41.0
27	36.77162264251174	39.0	35.0	40.0	30.0	41.0
28	36.589935849860375	39.0	35.0	40.0	30.0	41.0
29	36.430417095943255	39.0	35.0	40.0	30.0	41.0
30	36.260489970379275	38.0	35.0	40.0	30.0	41.0
31	36.015407475210075	38.0	35.0	40.0	29.0	41.0
32	35.872604330172734	38.0	35.0	40.0	29.0	41.0
33	35.6676275481407	38.0	34.0	40.0	27.0	41.0
34	35.55827886181644	38.0	34.0	40.0	27.0	41.0
35	35.331137105791285	38.0	34.0	40.0	26.0	41.0
36	35.19237584350615	38.0	34.0	40.0	25.0	41.0
37	35.0332647001585	38.0	33.0	40.0	25.0	41.0
38	34.9771620599351	38.0	33.0	40.0	25.0	41.0
39	34.89351888978731	38.0	33.0	40.0	24.0	41.0
40	34.785022201495494	38.0	33.0	40.0	24.0	41.0
41	34.593875574575115	38.0	33.0	40.0	23.0	41.0
42	34.38330563999201	38.0	33.0	40.0	23.0	41.0
43	34.065285626210894	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	17.0
15	23.0
16	44.0
17	137.0
18	330.0
19	836.0
20	1824.0
21	3457.0
22	6466.0
23	11326.0
24	17544.0
25	26819.0
26	38420.0
27	53894.0
28	72727.0
29	94437.0
30	119435.0
31	147120.0
32	179037.0
33	218594.0
34	266934.0
35	322875.0
36	407836.0
37	536424.0
38	699378.0
39	689563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50377141934802	21.256176091852335	13.460828266720334	23.779224222079314
2	19.08741203554487	23.257707440833897	34.95554337604731	22.699337147573925
3	19.698649113191617	24.918235260766053	32.0905467549244	23.29256887111793
4	13.885243833357766	16.334968032196127	36.29749135615305	33.482296778293055
5	12.715329988338661	38.436961595218186	35.11419480822638	13.733513608216775
6	32.66942527420495	37.249323330699355	13.763113899571497	16.3181374955242
7	26.608567688127856	32.42284131128014	21.64634317643051	19.3222478241615
8	27.01954947283899	34.47491534929621	18.985483853666786	19.520051324198022
9	24.755599665125953	14.37473406985873	21.365255336352785	39.50441092866253
10	16.292138274990027	27.381086767418328	33.747499043545375	22.57927591404627
11	33.55855775325871	22.295302109410237	23.467233575669226	20.678906561661826
12	21.148424901838897	26.768547174793934	31.006573358920512	21.076454564446653
13	31.327451754589834	20.748297408608142	26.165814753765925	21.758436083036095
14	20.778076476528426	22.50201889821535	27.405834551993593	29.314070073262638
15	23.008646145500627	29.183154548254603	25.20195367030843	22.606245635936336
16	22.38517564286776	28.10896278433774	26.153070538592498	23.352791034202
17	22.11427806702691	26.551052713036537	26.767091422760096	24.567577797176455
18	23.23500281700788	25.49800766287439	28.94009503762225	22.326894482495476
19	23.076428003356913	24.713638251238283	29.930312894745043	22.27962085065976
20	23.839906096331966	24.49568407831231	29.86028356006458	21.804126265291142
21	22.875176669556037	25.276222563543577	30.27272109680955	21.575879670090835
22	21.85931714503019	25.56083485591375	29.8639867889226	22.715861210133465
23	22.748653940158885	25.810355862415594	30.561394171168853	20.879596026256657
24	22.700205490892355	25.889196327827186	28.272466723296276	23.138131457984187
25	22.695046510000505	25.960783572578944	29.350080781468122	21.99408913595243
26	22.525872799962816	26.447004751881114	29.34285310031766	21.684269347838413
27	21.58916021496094	26.60310223312362	29.00700855216011	22.800728999755332
28	21.980706433044855	26.135141803017852	29.223660210108438	22.66049155382886
29	22.35672462943449	26.100663465374303	28.720659573153075	22.82195233203814
30	22.220215951875392	26.310547153340746	30.050782760422546	21.418454134361316
31	22.872801495185037	26.035486637473305	29.850987178655508	21.24072468868615
32	22.398430648228505	25.88281145048579	29.05525268535171	22.663505215934
33	22.131823709961076	26.023917239730693	29.538383584020643	22.305875466287592
34	21.583567062409877	26.43515441953548	29.968826474868354	22.01245204318629
35	21.18956905142687	28.299078892055217	28.91866738926452	21.592684667253394
36	23.131261329964843	26.170207549376812	28.922370618122528	21.77616050253582
37	22.131083064189472	26.78203203573897	28.830121910294004	22.256762989777556
38	22.021569648029807	25.603383678516828	29.981979322191638	22.393067351261728
39	21.610025994112632	25.401008606303865	30.610225713075874	22.378739686507632
40	20.64966382343822	26.116778895783987	30.255405309459682	22.97815197131811
41	22.013907795220128	25.011377851422374	30.28490344277694	22.68981091058056
42	20.65088971988777	26.799705376219958	29.837655554766666	22.711749349125604
43	21.18108993431749	26.415591155361433	29.190407768914433	23.212911141406643
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	246.0
1	424.5
2	603.0
3	1479.5
4	2356.0
5	2356.0
6	3871.5
7	5387.0
8	6009.5
9	6632.0
10	10037.0
11	13442.0
12	13442.0
13	25145.0
14	36848.0
15	59035.0
16	81222.0
17	75104.0
18	68986.0
19	68986.0
20	74387.5
21	79789.0
22	64632.0
23	49475.0
24	56049.5
25	62624.0
26	62624.0
27	70198.5
28	77773.0
29	86754.5
30	95736.0
31	106933.5
32	118131.0
33	118131.0
34	132356.0
35	146581.0
36	160695.0
37	174809.0
38	186962.5
39	199116.0
40	199116.0
41	206326.0
42	213536.0
43	226295.5
44	239055.0
45	251628.0
46	264201.0
47	264201.0
48	329926.5
49	395652.0
50	375851.5
51	356051.0
52	329917.0
53	303783.0
54	303783.0
55	273750.0
56	243717.0
57	209223.0
58	174729.0
59	156615.5
60	138502.0
61	138502.0
62	123319.0
63	108136.0
64	94919.5
65	81703.0
66	70410.0
67	59117.0
68	59117.0
69	50893.0
70	42669.0
71	36270.0
72	29871.0
73	24582.5
74	19294.0
75	19294.0
76	15339.0
77	11384.0
78	9063.0
79	6742.0
80	5264.5
81	3787.0
82	3787.0
83	2860.0
84	1933.0
85	1451.0
86	969.0
87	736.0
88	503.0
89	503.0
90	390.5
91	278.0
92	179.5
93	81.0
94	61.0
95	41.0
96	41.0
97	24.0
98	7.0
99	6.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3915502.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.85984367486918
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.56611780930814	38.38728234304152
2	12.081234542525971	11.805744622944973
3	3.9867496221538867	5.843758899278481
4	1.7257159242524047	3.3727284114481955
5	0.9251817929989671	2.2602118888382363
6	0.533196097948483	1.5631126796287875
7	0.36152549305682347	1.236485535266548
8	0.24532182486100046	0.9589108810193696
9	0.2041355704658876	0.897662885529916
>10	1.1542196972850824	10.86461784949438
>50	0.12579544881101554	4.2766090035905595
>100	0.07871644434123858	7.51052138091364
>500	0.007274773806891709	2.443794707582056
>1k	0.004239256662238764	3.997853479153066
>5k	3.1401901201768623E-4	1.2325734651954519
>10k+	2.616825100147385E-4	3.3481319670747682
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40308	1.029446543508342	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	35690	0.9115050892580313	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28782	0.7350781585605115	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14639	0.3738728776029229	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11513	0.29403637132607774	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9960	0.254373513281311	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8936	0.22822105569094334	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8299	0.21195238822506027	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7986	0.20395852179362953	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7140	0.18235209687033743	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5880	0.15017231506968964	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4503	0.11500441067326744	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4396	0.11227168317114894	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4372	0.11165873494637468	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4306	0.10997312732824553	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4032	0.10297530176207291	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	2.553950936559348E-5	0.0	0.0	0.0	0.0
7	2.553950936559348E-5	0.0	0.0	2.553950936559348E-5	0.0
8	2.553950936559348E-5	0.0	0.0	2.553950936559348E-5	0.0
9	2.553950936559348E-5	0.0	0.0	1.5323705619356087E-4	0.0
10	2.553950936559348E-5	0.0	0.0	2.0431607492474784E-4	0.0
11	1.0215803746237392E-4	0.0	0.0	3.5755313111830874E-4	0.0
12	1.0215803746237392E-4	0.0	0.0	4.086321498494957E-4	0.0
13	1.0215803746237392E-4	0.0	0.0	4.5971116858068263E-4	0.0
14	1.276975468279674E-4	0.0	0.0	5.874087154086501E-4	0.0
15	1.276975468279674E-4	0.0	0.0	8.172642996989914E-4	0.0
16	1.276975468279674E-4	0.0	0.0	0.001225896449548487	0.0
17	1.276975468279674E-4	0.0	0.0	0.0020942397679786654	0.0
18	1.276975468279674E-4	0.0	0.0	0.0024773324084625676	0.0
19	1.5323705619356087E-4	0.0	0.0	0.0034222942549895262	0.0
20	1.5323705619356087E-4	0.0	0.0	0.006027324210280061	0.0
21	1.7877656555915437E-4	0.0	0.0	0.010317961783699765	0.0
22	1.7877656555915437E-4	0.0	0.0	0.016983773728119662	0.0
23	1.7877656555915437E-4	0.0	0.0	0.02112117424534581	0.0
24	2.8093460302152826E-4	0.0	0.0	0.027174037964991463	0.0
25	2.8093460302152826E-4	0.0	0.0	0.03177114965079829	0.0
26	2.8093460302152826E-4	0.0	0.0	0.03889667276379887	0.0
27	2.8093460302152826E-4	0.0	0.0	0.079095860505243	0.0
28	2.8093460302152826E-4	0.0	0.0	0.1657514157827017	0.0
29	2.8093460302152826E-4	0.0	0.0	0.27115297093450597	0.0
30	3.0647411238712174E-4	0.0	0.0	0.41506810620962525	0.0
31	3.830926404839022E-4	0.0	0.0	0.5996421403947693	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11560	0.0	23.669119	1
GTATTGG	1780	0.0	16.421349	1
TAATACT	955	0.0	15.884816	4
GTATTAG	940	0.0	15.744681	1
GTATCAA	17415	0.0	15.658341	2
TTGGACC	2550	0.0	14.72745	4
ATTGGAC	2195	0.0	14.496584	3
TTAACGG	555	0.0	14.333333	35
TATACTG	540	0.0	14.046297	5
TGGACCC	2640	0.0	13.875	5
ATAATAC	480	0.0	13.874999	3
ACCGTTA	470	0.0	13.776596	8
GGACCCT	2570	0.0	13.605059	6
GGACCGT	630	0.0	13.507937	6
CTAATAC	865	0.0	13.4739895	3
TCTATAC	400	0.0	13.4125	3
TTCGTTA	255	1.6370905E-11	13.058825	30
ATTAGAG	865	0.0	13.046244	3
TACTGGT	995	0.0	13.015076	7
CCGTTTA	485	0.0	12.969072	27
>>END_MODULE