FastQCFastQC Report
Wed 25 May 2016
SRR2088439_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088439_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3961311
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT333880.842852277945357No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT291720.7364228660663098No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT234220.5912689006240611No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124440.31413842538492937No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG80420.2030135982759243No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76660.19352179114439638No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG69230.17476537439246753No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69170.17461390938504953No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA60590.15295441332427573No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG54000.13631850667619885No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG46480.11733489241314303No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42570.1074644227630701No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93250.023.1919571
CTCTAAT13850.017.0974731
CTAATAC16050.016.7133963
TAATACT16650.016.6666664
AATACTG16550.016.655595
GTATTAG13750.016.5490911
ATGCTAG13700.016.06934415
TATACCG2200.015.9772735
GGTAAAC13850.015.62815935
ATACTGG16100.015.627336
TACTGGT15450.015.326867
TGCTAGA14550.015.13058516
TCTAATA15900.015.1257862
GTATTGG15800.015.104431
GTATCAA144050.015.0260322
GATGCTA15050.014.873754514
TTAACGG9450.014.48677335
GCTAGAG15200.014.48355317
GTGATGC15950.013.91849412
TAACGGC9850.013.89847736
Produced by FastQC (version 0.11.5)