##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088439_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3961311
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.25733349388624	31.0	31.0	33.0	30.0	34.0
2	31.48578488283298	31.0	31.0	34.0	30.0	34.0
3	31.59817090857042	31.0	31.0	34.0	30.0	34.0
4	35.32562224980568	37.0	35.0	37.0	33.0	37.0
5	35.17406989756674	37.0	35.0	37.0	32.0	37.0
6	35.229105970220466	37.0	35.0	37.0	32.0	37.0
7	35.08098379551618	37.0	35.0	37.0	32.0	37.0
8	35.110000704312284	37.0	35.0	37.0	32.0	37.0
9	36.645933126684575	39.0	35.0	39.0	32.0	39.0
10	36.46872588393085	38.0	35.0	39.0	32.0	39.0
11	36.507953048877	38.0	35.0	39.0	32.0	39.0
12	36.36284704735377	38.0	35.0	39.0	32.0	39.0
13	36.38336474970029	38.0	35.0	39.0	32.0	39.0
14	37.356414328488725	39.0	36.0	41.0	32.0	41.0
15	37.36632241194897	39.0	36.0	41.0	32.0	41.0
16	37.2276970932098	39.0	36.0	40.0	32.0	41.0
17	37.275803389332474	39.0	36.0	40.0	32.0	41.0
18	37.14068675749014	39.0	36.0	40.0	31.0	41.0
19	37.228348645183374	39.0	36.0	40.0	31.0	41.0
20	37.12716295186114	39.0	36.0	40.0	31.0	41.0
21	36.96652951510245	39.0	36.0	40.0	31.0	41.0
22	36.88054005353278	39.0	36.0	40.0	31.0	41.0
23	36.69895875380651	39.0	35.0	40.0	30.0	41.0
24	36.901813818708	39.0	36.0	40.0	31.0	41.0
25	36.8657111244232	39.0	36.0	40.0	30.0	41.0
26	36.697761927806226	39.0	35.0	40.0	30.0	41.0
27	36.63749248670453	39.0	35.0	40.0	30.0	41.0
28	36.46080375915953	39.0	35.0	40.0	30.0	41.0
29	36.30397032699528	38.0	35.0	40.0	30.0	41.0
30	36.153088207414164	38.0	35.0	40.0	29.0	41.0
31	35.93006179014978	38.0	35.0	40.0	29.0	41.0
32	35.79396366505937	38.0	34.0	40.0	28.0	41.0
33	35.6158577299283	38.0	34.0	40.0	27.0	41.0
34	35.518311993176	38.0	34.0	40.0	27.0	41.0
35	35.298690761720046	38.0	34.0	40.0	26.0	41.0
36	35.17830915068269	38.0	34.0	40.0	25.0	41.0
37	35.031214666053735	38.0	33.0	40.0	25.0	41.0
38	34.99521951192421	38.0	33.0	40.0	25.0	41.0
39	34.943563128469336	38.0	33.0	40.0	25.0	41.0
40	34.85571771567544	38.0	33.0	40.0	24.0	41.0
41	34.685665427430465	38.0	33.0	40.0	24.0	41.0
42	34.50566239308148	38.0	33.0	40.0	23.0	41.0
43	34.19074518511675	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	1.0
12	2.0
13	3.0
14	7.0
15	30.0
16	61.0
17	149.0
18	341.0
19	868.0
20	2000.0
21	3678.0
22	6783.0
23	11671.0
24	18543.0
25	27663.0
26	40232.0
27	55978.0
28	75288.0
29	98634.0
30	124293.0
31	152231.0
32	186420.0
33	226670.0
34	273445.0
35	332475.0
36	414740.0
37	539995.0
38	718448.0
39	650661.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.6476037857164	21.890278243743044	13.385391856382899	24.076726114157662
2	19.244007854975283	23.203782788071926	34.790401460526574	22.76180789642621
3	19.350462511022233	25.295287343003363	32.03689384650688	23.317356299467527
4	13.963559033865302	16.791385478191437	36.97912130605246	32.26593418189079
5	12.513458297013285	38.65422331142392	35.58208381013255	13.25023458143024
6	32.256038468072816	38.31090262794312	14.038079817514959	15.394979086469101
7	26.55156335869615	32.433959363453155	22.15683141263082	18.857645865219872
8	26.602329380349083	34.978041360549575	19.11397514610693	19.30565411299441
9	24.842205017480325	14.246874330240669	21.190812839486725	39.720107812792286
10	16.133270020960232	27.456844463865625	34.22207950852634	22.1878060066478
11	33.883201798596474	22.472383511418315	23.30190686871089	20.34250782127432
12	20.906311067219917	27.096080060363853	30.77968379660168	21.21792507581455
13	30.37363135588193	21.179705405609404	25.996216908998054	22.45044632951061
14	20.74063359327253	22.307690560019143	27.307550454887284	29.644125391821042
15	23.17780653929974	29.427606163717012	24.95317837958191	22.441408917401333
16	22.588102777085666	28.207075889774874	25.983468604207044	23.221352728932416
17	22.104777938414834	26.91714939826739	26.866560085789782	24.111512577527996
18	22.670954136143312	26.279860379556162	28.804277169856142	22.244908314444384
19	23.106390788302157	25.25966782209223	29.467491949003747	22.166449440601863
20	23.510953823115628	25.175276568792505	29.523130095061962	21.7906395130299
21	22.776777687992688	25.902788243589054	29.646473099436022	21.67396096898224
22	21.911407612277856	26.33532181643905	29.1548429295251	22.598427641757993
23	22.56581217682732	26.365463352915235	30.06093184806747	21.007792622189978
24	22.747696406568434	26.630476627560927	27.658797807089623	22.96302915878102
25	22.676204923067136	26.29215428932492	28.922975247336048	22.108665540271893
26	22.405234024796336	27.04655100293817	28.900659402909795	21.6475555693557
27	21.829717484943746	27.02718872615657	28.31188967490813	22.831204113991554
28	21.871117920304666	26.643755059877904	28.9789920559128	22.506134963904625
29	22.740426086212366	26.579811582579605	28.336603715285168	22.34315861592286
30	22.359769278403032	26.54911467440956	29.472187364233708	21.6189286829537
31	22.5984781300938	26.538310170547074	29.493594418615455	21.36961728074367
32	22.062948352199562	26.233007203928192	28.84635919775044	22.8576852461218
33	21.91221542565075	26.386012105588275	29.360279967919713	22.341492500841262
34	21.79664762499082	26.65486249375522	29.657731998320756	21.8907578829332
35	21.432828677172786	28.238151460463467	28.561125344614446	21.7678945177493
36	23.19732028108876	26.506149100638655	28.41708212255993	21.879448495712655
37	22.097962013081023	26.727187034797318	28.594018495392053	22.580832456729603
38	22.0161456649074	25.77896560002484	29.712814772685103	22.492073962382655
39	21.553344334741706	25.886480511123715	30.161504612993024	22.39867054114156
40	20.81558352777654	26.523845262338657	29.957758933847913	22.702812276036898
41	21.7771338832018	25.188832686956413	30.13297870326263	22.901054726579158
42	20.65023422801189	26.89624722724371	29.74792941023818	22.705589134506226
43	21.195937405571033	26.151216099922475	29.2507707675565	23.40207572694999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	217.0
1	407.0
2	597.0
3	1234.5
4	1872.0
5	1872.0
6	3166.0
7	4460.0
8	5078.0
9	5696.0
10	8489.5
11	11283.0
12	11283.0
13	21610.5
14	31938.0
15	50078.5
16	68219.0
17	63524.0
18	58829.0
19	58829.0
20	64406.0
21	69983.0
22	58606.0
23	47229.0
24	53988.5
25	60748.0
26	60748.0
27	69668.5
28	78589.0
29	89247.5
30	99906.0
31	115856.0
32	131806.0
33	131806.0
34	151715.5
35	171625.0
36	188752.0
37	205879.0
38	219002.0
39	232125.0
40	232125.0
41	238653.0
42	245181.0
43	259132.0
44	273083.0
45	273403.5
46	273724.0
47	273724.0
48	319400.5
49	365077.0
50	350208.5
51	335340.0
52	311585.5
53	287831.0
54	287831.0
55	261305.5
56	234780.0
57	202348.0
58	169916.0
59	153787.0
60	137658.0
61	137658.0
62	122918.5
63	108179.0
64	94440.5
65	80702.0
66	69086.0
67	57470.0
68	57470.0
69	49386.0
70	41302.0
71	34591.0
72	27880.0
73	22892.5
74	17905.0
75	17905.0
76	14379.5
77	10854.0
78	8483.5
79	6113.0
80	4797.5
81	3482.0
82	3482.0
83	2647.5
84	1813.0
85	1433.5
86	1054.0
87	765.0
88	476.0
89	476.0
90	383.5
91	291.0
92	210.0
93	129.0
94	89.5
95	50.0
96	50.0
97	33.5
98	17.0
99	10.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3961311.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.68014667281655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.73444704046399	36.753355360519656
2	11.887145852034786	11.097874237882932
3	3.915751044665019	5.48363499297593
4	1.6959677593476001	3.1667209503485596
5	0.8978239089269621	2.0955275877536037
6	0.5574338549094423	1.5612656464539785
7	0.37297659522161686	1.218742151933096
8	0.2570881832257272	0.9600731280659903
9	0.19698252824789506	0.8275655979534571
>10	1.2379684979150725	11.005640338211357
>50	0.13238186620835093	4.291245327492564
>100	0.09458949232861832	8.540886122542924
>500	0.012057065886250598	3.9103703208771137
>1k	0.006843199543981453	5.5521224579579
>5k	3.2586664495149777E-4	1.0396301576436189
>10k+	2.172444299676652E-4	2.495345621387344
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	33388	0.842852277945357	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29172	0.7364228660663098	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23422	0.5912689006240611	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12444	0.31413842538492937	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8042	0.2030135982759243	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7666	0.19352179114439638	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6923	0.17476537439246753	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6917	0.17461390938504953	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6059	0.15295441332427573	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5400	0.13631850667619885	No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG	4648	0.11733489241314303	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4257	0.1074644227630701	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.524416790299979E-5	0.0
3	0.0	0.0	0.0	2.524416790299979E-5	0.0
4	0.0	0.0	0.0	2.524416790299979E-5	0.0
5	0.0	0.0	0.0	2.524416790299979E-5	0.0
6	2.524416790299979E-5	0.0	0.0	2.524416790299979E-5	0.0
7	2.524416790299979E-5	0.0	0.0	2.524416790299979E-5	0.0
8	2.524416790299979E-5	0.0	0.0	2.524416790299979E-5	0.0
9	2.524416790299979E-5	0.0	0.0	2.5244167902999787E-4	0.0
10	2.524416790299979E-5	0.0	0.0	3.7866251854499686E-4	0.0
11	2.524416790299979E-5	0.0	0.0	4.291508543509964E-4	0.0
12	2.524416790299979E-5	0.0	0.0	6.05860029671995E-4	0.0
13	2.524416790299979E-5	0.0	0.0	6.563483654779945E-4	0.0
14	1.0097667161199916E-4	0.0	0.0	7.320808691869939E-4	0.0
15	1.0097667161199916E-4	0.0	0.0	8.835458766049926E-4	2.524416790299979E-5
16	1.0097667161199916E-4	0.0	0.0	0.0011107433877319908	2.524416790299979E-5
17	1.0097667161199916E-4	0.0	0.0	0.0015903825778889867	2.524416790299979E-5
18	1.0097667161199916E-4	0.0	0.0	0.0018175800890159848	2.524416790299979E-5
19	1.2622083951499894E-4	0.0	2.524416790299979E-5	0.00239819595078498	2.524416790299979E-5
20	1.2622083951499894E-4	0.0	2.524416790299979E-5	0.0036604043459349694	2.524416790299979E-5
21	1.2622083951499894E-4	0.0	2.524416790299979E-5	0.006613971990585945	5.048833580599958E-5
22	1.2622083951499894E-4	0.0	2.524416790299979E-5	0.010249132168617914	5.048833580599958E-5
23	1.5146500741799874E-4	0.0	2.524416790299979E-5	0.012899769798432893	5.048833580599958E-5
24	1.7670917532099851E-4	0.0	2.524416790299979E-5	0.016913592495009858	5.048833580599958E-5
25	1.7670917532099851E-4	0.0	2.524416790299979E-5	0.020296310994011833	5.048833580599958E-5
26	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.026001492940089785	5.048833580599958E-5
27	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.06146954884380449	5.048833580599958E-5
28	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.13525825162427288	5.048833580599958E-5
29	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.22384003679589914	5.048833580599958E-5
30	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.34629949529335113	5.048833580599958E-5
31	2.0195334322399832E-4	0.0	2.524416790299979E-5	0.5103360983270437	5.048833580599958E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9325	0.0	23.191957	1
CTCTAAT	1385	0.0	17.097473	1
CTAATAC	1605	0.0	16.713396	3
TAATACT	1665	0.0	16.666666	4
AATACTG	1655	0.0	16.65559	5
GTATTAG	1375	0.0	16.549091	1
ATGCTAG	1370	0.0	16.069344	15
TATACCG	220	0.0	15.977273	5
GGTAAAC	1385	0.0	15.628159	35
ATACTGG	1610	0.0	15.62733	6
TACTGGT	1545	0.0	15.32686	7
TGCTAGA	1455	0.0	15.130585	16
TCTAATA	1590	0.0	15.125786	2
GTATTGG	1580	0.0	15.10443	1
GTATCAA	14405	0.0	15.026032	2
GATGCTA	1505	0.0	14.8737545	14
TTAACGG	945	0.0	14.486773	35
GCTAGAG	1520	0.0	14.483553	17
GTGATGC	1595	0.0	13.918494	12
TAACGGC	985	0.0	13.898477	36
>>END_MODULE