FastQCFastQC Report
Wed 25 May 2016
SRR2088438_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088438_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3377056
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT422951.2524222281182191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT343661.01763192555883No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT291970.8645696132963149No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152790.4524354941108468No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG85590.2534456046923711No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA81170.24035728160859637No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG79180.234464575061829No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69800.20668890299716677No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA69450.20565249732311222No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG64160.1899879658495447No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT46180.13674632579382753No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45860.13579875489183477No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39250.11622549344754722No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.11187258961651805No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC36700.10867453782229256No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA35460.10500270057707069No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34870.10325561672652156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA119800.021.125211
GTATTAG6250.017.4641
GTATTGG15450.016.284791
ACCGTTA6000.015.7249998
TCTAACG2201.8189894E-1215.1363642
TAATACT7400.015.04
TATAGCG750.00410659814.7999995
CCGTTTA6150.014.73983827
TCTATAC2650.014.66037753
CGTTATT3850.014.41558410
CGTTTAC6300.014.38888828
AATTACG1551.2122655E-714.32258116
TTGGACC20950.014.1288784
CCGTTAT6700.014.0820899
CTCTAAT6450.014.0542641
ATAACGC1854.9094524E-914.03
TGGACCC20300.013.8522175
TGTTACG4150.013.81927716
ATTGGAC18000.013.6694443
AACCGTA950.001245948513.6315797
Produced by FastQC (version 0.11.5)