##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088438_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3377056
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.310921109984555	31.0	31.0	34.0	30.0	34.0
2	31.523993679702084	31.0	31.0	34.0	30.0	34.0
3	31.629710907962437	31.0	31.0	34.0	30.0	34.0
4	35.339737333345965	37.0	35.0	37.0	33.0	37.0
5	35.20646118986478	37.0	35.0	37.0	33.0	37.0
6	35.26782617759374	37.0	35.0	37.0	32.0	37.0
7	35.10109308225863	37.0	35.0	37.0	32.0	37.0
8	35.1466460727924	37.0	35.0	37.0	32.0	37.0
9	36.67195657993234	39.0	35.0	39.0	32.0	39.0
10	36.51887472401997	38.0	35.0	39.0	32.0	39.0
11	36.56141799247629	38.0	35.0	39.0	32.0	39.0
12	36.413698203405566	38.0	35.0	39.0	32.0	39.0
13	36.43916950148295	38.0	35.0	39.0	32.0	39.0
14	37.363908682592175	39.0	36.0	41.0	32.0	41.0
15	37.38252756246861	39.0	36.0	41.0	32.0	41.0
16	37.239941830991256	39.0	36.0	40.0	32.0	41.0
17	37.33294502667412	39.0	36.0	40.0	32.0	41.0
18	37.21230089166422	39.0	36.0	40.0	31.0	41.0
19	37.299451652563654	39.0	36.0	40.0	31.0	41.0
20	37.212301483896034	39.0	36.0	40.0	31.0	41.0
21	37.063539366833126	39.0	36.0	40.0	31.0	41.0
22	36.97551269508116	39.0	36.0	40.0	31.0	41.0
23	36.79204075976235	39.0	35.0	40.0	30.0	41.0
24	36.9713481209669	39.0	36.0	40.0	31.0	41.0
25	36.93751895141804	39.0	36.0	40.0	31.0	41.0
26	36.75668540882947	39.0	35.0	40.0	30.0	41.0
27	36.6793180213772	39.0	35.0	40.0	30.0	41.0
28	36.490218107132364	39.0	35.0	40.0	30.0	41.0
29	36.3083164744677	38.0	35.0	40.0	30.0	41.0
30	36.133513036206686	38.0	35.0	40.0	30.0	41.0
31	35.87196214691139	38.0	35.0	40.0	29.0	41.0
32	35.71835113187344	38.0	34.0	40.0	28.0	41.0
33	35.50953552443312	38.0	34.0	40.0	27.0	41.0
34	35.390475609524984	38.0	34.0	40.0	27.0	41.0
35	35.16120401912198	38.0	34.0	40.0	26.0	41.0
36	35.00593001715103	38.0	33.0	40.0	25.0	41.0
37	34.82764662475245	38.0	33.0	40.0	25.0	41.0
38	34.747874183904564	38.0	33.0	40.0	24.0	41.0
39	34.65054799209726	38.0	33.0	40.0	24.0	41.0
40	34.525459157325194	38.0	33.0	40.0	23.0	41.0
41	34.31032887817081	38.0	33.0	40.0	22.0	41.0
42	34.080566919826026	38.0	33.0	40.0	21.0	41.0
43	33.743949463674866	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	2.0
14	14.0
15	23.0
16	46.0
17	134.0
18	342.0
19	767.0
20	1609.0
21	3360.0
22	6185.0
23	10186.0
24	16256.0
25	24339.0
26	35422.0
27	48814.0
28	65796.0
29	86322.0
30	107776.0
31	131397.0
32	160497.0
33	194120.0
34	234031.0
35	282730.0
36	354213.0
37	465203.0
38	585117.0
39	562351.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.55338259122739	21.41400675618053	13.875310329470402	23.157300323121678
2	19.145522016809906	23.8138485118399	34.60034420513015	22.440285266220044
3	19.963246093638958	24.94915689878995	32.419835501691416	22.667761505879678
4	14.053779386542598	16.4798273999602	35.98237636568656	33.484016847810636
5	12.713114618176306	38.76308240076563	34.6507135208892	13.873089460168858
6	32.46650336861456	37.36426046828954	13.510495532203198	16.65874063089271
7	26.186447604066977	32.93113883808856	21.437133408507293	19.445280149337172
8	27.40472766812277	34.25009831048108	18.816093070413995	19.52908095098216
9	24.835981399183193	14.393276273772186	21.497896392597575	39.27284593444704
10	16.61938682686932	27.675259160641698	33.104721982697356	22.60063202979163
11	33.36939038026021	22.692309514559426	23.18862938606881	20.749670719111556
12	21.288720115982677	27.298807008234395	30.630762415547743	20.781710460235185
13	31.562876067201728	21.026065306586567	26.050382344858953	21.360676281352752
14	20.726129504515175	22.97299778268409	27.651569888091878	28.64930282470886
15	22.983894848057005	29.528026778353688	25.34891337306814	22.139165000521164
16	22.006593909014242	28.233319198734048	26.46601063174552	23.29407626050619
17	21.94861441444856	26.787947845697552	26.98815181033421	24.275285929519676
18	23.049484521429317	25.494395118114717	29.37520135881667	22.080919001639298
19	23.02401855343826	24.894908464650868	30.344596003145934	21.736476978764934
20	23.71835113187344	24.70930301422304	30.188661366586757	21.383684487316764
21	22.5753437313447	25.550509082467094	30.623448352647987	21.25069883354022
22	21.548265708356627	25.991692172116775	30.132280897918186	22.32776122160841
23	22.415974150265793	26.08748566799011	30.969548624600833	20.526991557143262
24	22.354056314138706	26.15396368908304	28.753121061658437	22.738858935119822
25	22.291427799835123	26.235158670747538	29.77614229672235	21.697271232694987
26	22.031971042233234	26.847230250253475	29.805931556953745	21.31486715055954
27	21.21007173111728	26.957888764651816	29.36178730823534	22.470252195995567
28	21.560761799626658	26.64732240152369	29.538598116229046	22.2533176826206
29	21.83863696663603	26.62185643353264	29.081424767608237	22.458081832223094
30	21.72081244729137	26.828486113348433	30.542993660750668	20.907707778609534
31	22.27602977267774	26.4769965318905	30.389487174627845	20.85748652080392
32	21.867241763239935	26.38312778941184	29.424030871859983	22.325599575488237
33	21.56342684278851	26.43154274018553	29.945106033183933	22.059924383842024
34	20.94489993651275	26.92374660058939	30.454810343683963	21.676543119213896
35	20.62627922071769	28.67059355841301	29.41517700624449	21.28795021462481
36	22.609959680858122	26.489996020202213	29.401674120891098	21.498370178048575
37	21.561650147347276	27.008702254271178	29.3635047804952	22.066142817886348
38	21.362156860887115	25.813430396179392	30.669672045710822	22.154740697222667
39	20.90818156406053	25.7049927510826	31.317188699269423	22.069636985587447
40	19.990103806392312	26.331396340481177	31.033628106848095	22.644871746278415
41	21.103706897368596	25.25815384761165	31.15476912434973	22.48337013067003
42	19.948617967839443	26.938552395933023	30.62036874721651	22.49246088901102
43	20.228891673694484	26.655880151232314	30.080993622847828	23.03423455222537
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	320.0
1	510.0
2	700.0
3	1680.5
4	2661.0
5	2661.0
6	4142.0
7	5623.0
8	6347.0
9	7071.0
10	10700.5
11	14330.0
12	14330.0
13	27214.5
14	40099.0
15	63337.0
16	86575.0
17	79031.0
18	71487.0
19	71487.0
20	75016.5
21	78546.0
22	62730.5
23	46915.0
24	52402.5
25	57890.0
26	57890.0
27	63213.5
28	68537.0
29	75289.0
30	82041.0
31	90339.5
32	98638.0
33	98638.0
34	110003.5
35	121369.0
36	132664.5
37	143960.0
38	153978.0
39	163996.0
40	163996.0
41	170515.5
42	177035.0
43	189788.0
44	202541.0
45	213354.0
46	224167.0
47	224167.0
48	282203.0
49	340239.0
50	323442.0
51	306645.0
52	283633.0
53	260621.0
54	260621.0
55	233812.5
56	207004.0
57	178179.5
58	149355.0
59	133013.5
60	116672.0
61	116672.0
62	103717.5
63	90763.0
64	78959.0
65	67155.0
66	57691.5
67	48228.0
68	48228.0
69	41433.0
70	34638.0
71	29409.0
72	24180.0
73	19885.5
74	15591.0
75	15591.0
76	12671.0
77	9751.0
78	7553.5
79	5356.0
80	4267.0
81	3178.0
82	3178.0
83	2373.5
84	1569.0
85	1184.0
86	799.0
87	609.0
88	419.0
89	419.0
90	332.5
91	246.0
92	163.0
93	80.0
94	62.0
95	44.0
96	44.0
97	29.5
98	15.0
99	11.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3377056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.945555654057344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.43330179883063	36.82094934728479
2	11.953659577163803	11.223423818987976
3	4.0270578326203275	5.671574028101553
4	1.697032598199786	3.1867255314215024
5	0.9530415198733282	2.2370531855920364
6	0.5629813985687052	1.5857684747223715
7	0.3813855744418594	1.2533050397430456
8	0.26867475687727466	1.0090468601457938
9	0.19941434896994342	0.8425455676007481
>10	1.2759509957179178	11.568789345742006
>50	0.14004457550105096	4.554421196562168
>100	0.09448121743917479	8.675675683609903
>500	0.008100717765671974	2.6221775644784984
>1k	0.00424021945517	3.814484299528572
>5k	3.7972114523910445E-4	1.3350353379130329
>10k+	2.53147430159403E-4	3.5990247185661746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	42295	1.2524222281182191	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	34366	1.01763192555883	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	29197	0.8645696132963149	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15279	0.4524354941108468	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8559	0.2534456046923711	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8117	0.24035728160859637	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7918	0.234464575061829	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6980	0.20668890299716677	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6945	0.20565249732311222	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6416	0.1899879658495447	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4618	0.13674632579382753	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4586	0.13579875489183477	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3925	0.11622549344754722	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3778	0.11187258961651805	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3670	0.10867453782229256	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3546	0.10500270057707069	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3487	0.10325561672652156	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.9611590687273174E-5	0.0	0.0	0.0	0.0
2	2.9611590687273174E-5	0.0	0.0	0.0	0.0
3	5.922318137454635E-5	0.0	0.0	0.0	0.0
4	5.922318137454635E-5	0.0	0.0	2.9611590687273174E-5	0.0
5	5.922318137454635E-5	0.0	0.0	2.9611590687273174E-5	2.9611590687273174E-5
6	5.922318137454635E-5	0.0	0.0	5.922318137454635E-5	2.9611590687273174E-5
7	5.922318137454635E-5	0.0	0.0	5.922318137454635E-5	2.9611590687273174E-5
8	1.4805795343636588E-4	0.0	0.0	5.922318137454635E-5	2.9611590687273174E-5
9	1.7766954412363906E-4	0.0	0.0	1.7766954412363906E-4	2.9611590687273174E-5
10	2.0728113481091224E-4	0.0	0.0	2.9611590687273176E-4	5.922318137454635E-5
11	2.0728113481091224E-4	0.0	0.0	5.330086323709172E-4	5.922318137454635E-5
12	2.368927254981854E-4	0.0	0.0	7.995129485563758E-4	5.922318137454635E-5
13	2.368927254981854E-4	0.0	0.0	8.587361299309221E-4	5.922318137454635E-5
14	2.368927254981854E-4	0.0	0.0	0.001036405674054561	5.922318137454635E-5
15	2.368927254981854E-4	0.0	0.0	0.0014509679436763856	5.922318137454635E-5
16	2.368927254981854E-4	0.0	0.0	0.00186553021329821	5.922318137454635E-5
17	2.368927254981854E-4	0.0	0.0	0.002635431571167313	5.922318137454635E-5
18	2.368927254981854E-4	0.0	0.0	0.003227663384912776	5.922318137454635E-5
19	2.368927254981854E-4	0.0	5.922318137454635E-5	0.004086399514843698	5.922318137454635E-5
20	2.368927254981854E-4	0.0	5.922318137454635E-5	0.006692219495323738	5.922318137454635E-5
21	2.368927254981854E-4	0.0	5.922318137454635E-5	0.011459685595974718	5.922318137454635E-5
22	2.9611590687273176E-4	0.0	5.922318137454635E-5	0.0186553021329821	5.922318137454635E-5
23	2.9611590687273176E-4	0.0	5.922318137454635E-5	0.022978594373323984	5.922318137454635E-5
24	2.9611590687273176E-4	0.0	5.922318137454635E-5	0.029966929775520453	5.922318137454635E-5
25	2.9611590687273176E-4	0.0	5.922318137454635E-5	0.035741189959538726	5.922318137454635E-5
26	3.257274975600049E-4	0.0	5.922318137454635E-5	0.045542626477026144	5.922318137454635E-5
27	3.257274975600049E-4	0.0	5.922318137454635E-5	0.09955416789061242	5.922318137454635E-5
28	3.257274975600049E-4	0.0	5.922318137454635E-5	0.20725152322022494	5.922318137454635E-5
29	3.257274975600049E-4	0.0	5.922318137454635E-5	0.3483803644357689	5.922318137454635E-5
30	3.257274975600049E-4	0.0	5.922318137454635E-5	0.543283854339401	5.922318137454635E-5
31	3.257274975600049E-4	0.0	5.922318137454635E-5	0.7931168449679247	5.922318137454635E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11980	0.0	21.12521	1
GTATTAG	625	0.0	17.464	1
GTATTGG	1545	0.0	16.28479	1
ACCGTTA	600	0.0	15.724999	8
TCTAACG	220	1.8189894E-12	15.136364	2
TAATACT	740	0.0	15.0	4
TATAGCG	75	0.004106598	14.799999	5
CCGTTTA	615	0.0	14.739838	27
TCTATAC	265	0.0	14.6603775	3
CGTTATT	385	0.0	14.415584	10
CGTTTAC	630	0.0	14.388888	28
AATTACG	155	1.2122655E-7	14.322581	16
TTGGACC	2095	0.0	14.128878	4
CCGTTAT	670	0.0	14.082089	9
CTCTAAT	645	0.0	14.054264	1
ATAACGC	185	4.9094524E-9	14.0	3
TGGACCC	2030	0.0	13.852217	5
TGTTACG	415	0.0	13.819277	16
ATTGGAC	1800	0.0	13.669444	3
AACCGTA	95	0.0012459485	13.631579	7
>>END_MODULE