FastQCFastQC Report
Wed 25 May 2016
SRR2088437_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088437_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507952
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT206661.3704680255074433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT171681.1384977771175742No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT130710.8668047789319554No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71310.4728930363831209No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46080.3055800184621261No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG44850.29742326015682197No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG41260.27361613632264153No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39060.2590268125245366No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA35990.23866807431536285No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG30360.20133266841384873No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA24170.16028361645463515No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21530.1427764278969092No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17970.1191682493872484No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA17250.11439356159877768No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC16810.11147569683915667No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16720.11087886086559784No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC16170.1072315299160716No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA15480.1026557874521205No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.10166106082952243No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC15210.10086527953144397No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA15140.10040107377423155No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59200.023.8751
TCCGATC450.003826353520.5555558
CGACCTA701.2195176E-418.513
CCGTTTA2350.017.31914927
CGTTTAC2400.016.95833428
CGCCGTT2550.016.68627425
CTATACA1005.880238E-616.654
GTACTTA700.002592996715.8571421
GTTATCG1059.346884E-615.85714211
GTATCAA88800.015.8333332
GTATTGG6300.015.5634931
TAATACT3350.015.4626874
ACCGTTA2650.015.3584918
CTATATC1101.4523872E-515.1363642
TCTATAC1353.9742372E-715.0740753
CCGTTAT2950.015.0508479
GCGTTAT750.004105196314.811
TGGACTA1001.0937233E-414.7999995
GACCGTT2750.014.7999997
GTAGCAC1001.0937233E-414.7999993
Produced by FastQC (version 0.11.5)