##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088437_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1507952
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.034056123802348	31.0	31.0	33.0	30.0	34.0
2	31.25646704934905	31.0	31.0	34.0	28.0	34.0
3	31.345662196144175	31.0	31.0	34.0	28.0	34.0
4	35.093498997315564	35.0	35.0	37.0	32.0	37.0
5	34.93960616783558	35.0	35.0	37.0	32.0	37.0
6	35.02484163952168	36.0	35.0	37.0	32.0	37.0
7	34.84715428607807	35.0	35.0	37.0	32.0	37.0
8	34.9022767302938	35.0	35.0	37.0	32.0	37.0
9	36.37493965325156	38.0	35.0	39.0	32.0	39.0
10	36.1890027003512	38.0	35.0	39.0	32.0	39.0
11	36.24732551168737	38.0	35.0	39.0	32.0	39.0
12	36.063486105658534	38.0	35.0	39.0	31.0	39.0
13	36.11473972646344	38.0	35.0	39.0	31.0	39.0
14	36.93870627181767	38.0	36.0	40.0	31.0	41.0
15	36.98183960762677	38.0	36.0	40.0	31.0	41.0
16	36.81916931042898	38.0	36.0	40.0	31.0	41.0
17	36.916692308508495	38.0	36.0	40.0	31.0	41.0
18	36.78373051662122	38.0	36.0	40.0	31.0	41.0
19	36.876027884176686	39.0	36.0	40.0	31.0	41.0
20	36.78097976593419	39.0	36.0	40.0	30.0	41.0
21	36.60874484068459	38.0	35.0	40.0	30.0	41.0
22	36.51559665029126	38.0	35.0	40.0	30.0	41.0
23	36.306428851846746	38.0	35.0	40.0	30.0	41.0
24	36.49769356053773	38.0	35.0	40.0	30.0	41.0
25	36.46598167580931	38.0	35.0	40.0	30.0	41.0
26	36.2369604602799	38.0	35.0	40.0	30.0	41.0
27	36.165493994503805	38.0	35.0	40.0	30.0	41.0
28	35.94863961187094	38.0	34.0	40.0	29.0	41.0
29	35.758256894118645	38.0	34.0	40.0	27.0	41.0
30	35.56535486540685	38.0	34.0	40.0	27.0	41.0
31	35.26825920188441	38.0	34.0	40.0	26.0	41.0
32	35.11293927127654	38.0	33.0	40.0	26.0	41.0
33	34.88634120979978	38.0	33.0	40.0	25.0	41.0
34	34.746584108777995	37.0	33.0	40.0	25.0	41.0
35	34.477064256687214	37.0	33.0	40.0	24.0	41.0
36	34.33658697359067	37.0	33.0	40.0	24.0	41.0
37	34.141484609589696	37.0	33.0	40.0	23.0	41.0
38	34.05086103536452	37.0	33.0	40.0	23.0	41.0
39	33.93798874234724	37.0	33.0	40.0	22.0	41.0
40	33.78378091610343	37.0	32.0	40.0	21.0	41.0
41	33.5449881693847	37.0	32.0	40.0	20.0	41.0
42	33.27800022812397	37.0	31.0	40.0	18.0	41.0
43	32.94142917015926	36.0	31.0	40.0	17.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	5.0
14	4.0
15	13.0
16	44.0
17	91.0
18	222.0
19	513.0
20	1088.0
21	2179.0
22	3789.0
23	6208.0
24	9472.0
25	14224.0
26	19994.0
27	27128.0
28	35457.0
29	45200.0
30	55181.0
31	66670.0
32	79535.0
33	95026.0
34	113027.0
35	134211.0
36	166327.0
37	209919.0
38	251498.0
39	170926.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.47542627351534	22.43387057412968	14.348666270544422	22.742036881810563
2	19.996657718548068	24.634802699290162	33.39781372351375	21.97072585864802
3	20.819694526085712	25.115587233545895	32.36568537990599	21.699032860462403
4	14.855380012095875	17.04231964943181	35.16796290598109	32.93433743249122
5	12.674541364711875	39.305296189799144	34.26667427079907	13.75348817468991
6	32.809996604666466	37.33772693030017	13.025414602056298	16.826861862977072
7	26.2567376149904	33.09787048924634	21.02102719449956	19.6243647012637
8	27.920782624380617	34.13139145012573	18.477113329867265	19.470712595626384
9	24.866839262788208	14.261461903296658	21.269045699067345	39.60265313484779
10	16.734418602183624	27.8337108873492	32.881484291277175	22.55038621919
11	33.688008636879694	22.402503527963756	23.102525809840103	20.806962025316455
12	21.327469309367938	27.39490381656711	30.580747928316022	20.696878945748935
13	32.40646917143251	20.6346090591743	25.919525289929652	21.039396479463537
14	20.72546075737159	22.854308359947797	27.501604825617793	28.918626057062824
15	22.999936337496155	29.609231593578578	25.057627829002517	22.333204239922754
16	22.043075641664984	28.050163400426538	26.44759249631288	23.459168461595596
17	22.26111971733848	26.50190456990674	26.834673782719875	24.40230193003491
18	23.369311490020902	25.168108799219073	29.146484768745957	22.316094942014068
19	23.16598936836186	24.418814391970038	30.486248899169205	21.9289473404989
20	24.031865735779387	24.285719969866413	30.116409540887247	21.566004753466956
21	23.03707279807315	24.995755833076917	30.711653951849925	21.25551741700001
22	21.68371407047439	25.46606258024128	30.326960009337167	22.52326333994716
23	22.743296868865855	25.693987607032586	31.014780311309643	20.547935212791916
24	22.56630184515157	25.643057603955565	28.768820227699553	23.021820323193314
25	22.586527953144397	25.93311988710516	29.56546362218426	21.91488853756618
26	22.294210956316913	26.618685475399744	29.82117467930014	21.265928888983204
27	21.30843687332223	26.70336986853693	29.42142720723206	22.566766050908782
28	21.90527284688107	26.30541290438953	29.47089827792927	22.31841597080013
29	22.0083928400904	26.24254618184133	29.002580984010102	22.746479994058166
30	21.940287223996517	26.471068044606195	30.54255042600825	21.046094305389033
31	22.72651914649803	26.12682631807909	30.31608433159676	20.830570203826117
32	22.316824408203974	26.049303956624613	29.28607807145055	22.34779356372086
33	22.01257069190531	26.14751663182913	29.669047821150805	22.170864855114754
34	21.274218277504854	26.942701093934023	30.16064171803877	21.62243891052235
35	20.78222648996785	28.820347066750134	29.313466211126084	21.08396023215593
36	23.03541492036882	26.325639012382357	29.28488439950343	21.35406166774539
37	21.946388213948453	27.19768268486	29.090514817447772	21.76541428374378
38	21.922116884357063	25.649423854340192	30.40129924559933	22.027160015703416
39	21.42521777881524	25.511952635097142	31.1336833002642	21.92914628582342
40	20.308670302503	26.325572697274186	30.674782751705624	22.690974248517193
41	21.721513682133118	25.21937037783696	30.72597801521534	22.333137924814583
42	20.43738792746719	26.966242957335513	30.19227402463739	22.40409509055991
43	20.724598660965334	26.90304465924645	29.411811516546948	22.96054516324127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	90.0
1	154.5
2	219.0
3	482.5
4	746.0
5	746.0
6	1416.0
7	2086.0
8	2469.0
9	2852.0
10	4449.5
11	6047.0
12	6047.0
13	11963.5
14	17880.0
15	29542.0
16	41204.0
17	36825.5
18	32447.0
19	32447.0
20	34735.5
21	37024.0
22	29271.0
23	21518.0
24	23631.0
25	25744.0
26	25744.0
27	28258.5
28	30773.0
29	33332.5
30	35892.0
31	38940.5
32	41989.0
33	41989.0
34	46450.5
35	50912.0
36	55509.0
37	60106.0
38	64223.0
39	68340.0
40	68340.0
41	71033.5
42	73727.0
43	78772.0
44	83817.0
45	90762.0
46	97707.0
47	97707.0
48	126919.5
49	156132.0
50	148261.5
51	140391.0
52	130667.5
53	120944.0
54	120944.0
55	107569.5
56	94195.0
57	81338.5
58	68482.0
59	60630.5
60	52779.0
61	52779.0
62	46996.0
63	41213.0
64	36120.0
65	31027.0
66	27082.0
67	23137.0
68	23137.0
69	20209.0
70	17281.0
71	14807.5
72	12334.0
73	10128.0
74	7922.0
75	7922.0
76	6365.0
77	4808.0
78	3794.5
79	2781.0
80	2237.5
81	1694.0
82	1694.0
83	1283.5
84	873.0
85	655.5
86	438.0
87	325.5
88	213.0
89	213.0
90	162.0
91	111.0
92	82.5
93	54.0
94	35.5
95	17.0
96	17.0
97	11.0
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1507952.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.39398738900765
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.14448011183364	44.39415322556843
2	9.582810968522589	10.233289760090784
3	2.788029277648221	4.465920002727996
4	1.2003878641983443	2.5637397793169687
5	0.6836657985377825	1.8251821512711102
6	0.45589317986936523	1.460517281800769
7	0.31478649521830304	1.1765394309141193
8	0.23663611639337334	1.0107956651593217
9	0.2019992218701694	0.9706989514612664
>10	1.208729651223813	12.254379669448223
>50	0.10939809365292971	4.06366413726155
>100	0.06434653314175928	6.193643232341508
>500	0.004978415712617347	1.8914906321007532
>1k	0.0033604306060167092	3.6392404695827407
>5k	1.2446039281543367E-4	0.47388608711346214
>10k+	3.73381178446301E-4	3.3828595238410863
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20666	1.3704680255074433	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17168	1.1384977771175742	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13071	0.8668047789319554	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7131	0.4728930363831209	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4608	0.3055800184621261	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4485	0.29742326015682197	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4126	0.27361613632264153	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3906	0.2590268125245366	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3599	0.23866807431536285	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3036	0.20133266841384873	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2417	0.16028361645463515	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2153	0.1427764278969092	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1797	0.1191682493872484	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1725	0.11439356159877768	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1681	0.11147569683915667	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1672	0.11087886086559784	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1617	0.1072315299160716	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1548	0.1026557874521205	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1533	0.10166106082952243	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1521	0.10086527953144397	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1514	0.10040107377423155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.631510817320445E-5	0.0	0.0	0.0	0.0
7	6.631510817320445E-5	0.0	6.631510817320445E-5	0.0	0.0
8	1.326302163464089E-4	0.0	6.631510817320445E-5	0.0	0.0
9	1.326302163464089E-4	0.0	6.631510817320445E-5	1.9894532451961334E-4	0.0
10	1.326302163464089E-4	0.0	6.631510817320445E-5	1.9894532451961334E-4	0.0
11	1.326302163464089E-4	0.0	6.631510817320445E-5	3.3157554086602224E-4	0.0
12	1.326302163464089E-4	0.0	6.631510817320445E-5	3.978906490392267E-4	0.0
13	2.652604326928178E-4	0.0	6.631510817320445E-5	3.978906490392267E-4	0.0
14	2.652604326928178E-4	0.0	1.326302163464089E-4	6.631510817320445E-4	0.0
15	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0010610417307712712	0.0
16	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0012599870552908847	0.0
17	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0015915625961569068	0.0
18	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0023210287860621556	0.0
19	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0034483856250066315	0.0
20	2.652604326928178E-4	0.0	1.326302163464089E-4	0.005703099302895583	0.0
21	2.652604326928178E-4	0.0	1.326302163464089E-4	0.009284115144248622	0.0
22	2.652604326928178E-4	0.0	1.326302163464089E-4	0.016446146826954704	0.0
23	2.652604326928178E-4	0.0	1.326302163464089E-4	0.02022610799282736	0.0
24	2.652604326928178E-4	0.0	1.326302163464089E-4	0.026260782836588965	0.0
25	2.652604326928178E-4	0.0	1.326302163464089E-4	0.0313007310577525	0.0
26	2.652604326928178E-4	0.0	1.326302163464089E-4	0.03859539295680499	6.631510817320445E-5
27	2.652604326928178E-4	0.0	1.326302163464089E-4	0.08063917153861662	6.631510817320445E-5
28	2.652604326928178E-4	0.0	1.326302163464089E-4	0.17023088268061584	6.631510817320445E-5
29	2.652604326928178E-4	0.0	1.326302163464089E-4	0.28024764713996203	6.631510817320445E-5
30	2.652604326928178E-4	0.0	1.326302163464089E-4	0.4381439197003618	6.631510817320445E-5
31	2.652604326928178E-4	0.0	1.326302163464089E-4	0.614343162116566	6.631510817320445E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5920	0.0	23.875	1
TCCGATC	45	0.0038263535	20.555555	8
CGACCTA	70	1.2195176E-4	18.5	13
CCGTTTA	235	0.0	17.319149	27
CGTTTAC	240	0.0	16.958334	28
CGCCGTT	255	0.0	16.686274	25
CTATACA	100	5.880238E-6	16.65	4
GTACTTA	70	0.0025929967	15.857142	1
GTTATCG	105	9.346884E-6	15.857142	11
GTATCAA	8880	0.0	15.833333	2
GTATTGG	630	0.0	15.563493	1
TAATACT	335	0.0	15.462687	4
ACCGTTA	265	0.0	15.358491	8
CTATATC	110	1.4523872E-5	15.136364	2
TCTATAC	135	3.9742372E-7	15.074075	3
CCGTTAT	295	0.0	15.050847	9
GCGTTAT	75	0.0041051963	14.8	11
TGGACTA	100	1.0937233E-4	14.799999	5
GACCGTT	275	0.0	14.799999	7
GTAGCAC	100	1.0937233E-4	14.799999	3
>>END_MODULE