FastQCFastQC Report
Wed 25 May 2016
SRR2088436_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088436_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences699936
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT67340.9620879623279843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56630.8090739724774837No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47810.6830624514241301No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26240.3748914186439903No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14880.2125908654505555No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA14430.2061617062131395No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12830.18330247336899372No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG12330.17615896310519819No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA11340.16201481278288302No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG10260.1465848306130846No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7830.11186737073103828No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.11086727929410689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAC250.00549489329.625
TGTTACG502.7006396E-422.216
CGTGCGG603.7236074E-521.58333210
ACGAATG450.003824336520.55555518
GGTATCA22900.020.0349351
TCTAGAC701.2183329E-418.53
CTGGTCG609.2318986E-418.59
ACTAGGT500.007032200718.53
GTCAATC500.007032200718.516
ACCGTGT500.007032200718.58
ATTGCGT500.007032200718.56
ATACCGT701.2183329E-418.56
TAGTACT1205.162292E-918.54
TTAACGG752.0658108E-417.26666635
TAGGAGC752.0658108E-417.2666666
CCGTTTA1107.79688E-716.81818227
TACCCTC904.441962E-516.4444455
GTATTAG1606.2755134E-1016.18751
TTATACC803.3802004E-416.18754
ATTAGAG1151.2404198E-616.0869563
Produced by FastQC (version 0.11.5)