##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088436_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	699936
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.467156997211173	31.0	31.0	34.0	30.0	34.0
2	31.66581373154117	31.0	31.0	34.0	30.0	34.0
3	31.792986787363414	31.0	31.0	34.0	30.0	34.0
4	35.4807110935857	37.0	35.0	37.0	33.0	37.0
5	35.353245153842636	37.0	35.0	37.0	33.0	37.0
6	35.39744490924885	37.0	35.0	37.0	33.0	37.0
7	35.242826486993096	37.0	35.0	37.0	32.0	37.0
8	35.26985038632103	37.0	35.0	37.0	32.0	37.0
9	36.8330047318612	39.0	37.0	39.0	32.0	39.0
10	36.68437257120651	39.0	35.0	39.0	32.0	39.0
11	36.722891807250946	39.0	35.0	39.0	32.0	39.0
12	36.572163740684864	38.0	35.0	39.0	32.0	39.0
13	36.57604552416221	39.0	35.0	39.0	32.0	39.0
14	37.56539169295478	39.0	36.0	41.0	32.0	41.0
15	37.59676884743748	39.0	36.0	41.0	32.0	41.0
16	37.46133789375028	39.0	36.0	41.0	32.0	41.0
17	37.499515670004115	39.0	36.0	41.0	32.0	41.0
18	37.361414472180314	39.0	36.0	41.0	32.0	41.0
19	37.42960784986056	39.0	36.0	41.0	32.0	41.0
20	37.32554547844374	39.0	36.0	41.0	31.0	41.0
21	37.16750245736753	39.0	36.0	40.0	31.0	41.0
22	37.064867359301424	39.0	36.0	40.0	31.0	41.0
23	36.85088350934942	39.0	36.0	40.0	30.0	41.0
24	37.07359101403557	39.0	36.0	41.0	31.0	41.0
25	37.05649230786815	39.0	36.0	41.0	31.0	41.0
26	36.88353935216934	39.0	36.0	40.0	30.0	41.0
27	36.82551261829653	39.0	35.0	40.0	30.0	41.0
28	36.63783688794404	39.0	35.0	40.0	30.0	41.0
29	36.47772939240159	39.0	35.0	40.0	30.0	41.0
30	36.32862147396333	38.0	35.0	40.0	30.0	41.0
31	36.0509260846706	38.0	35.0	40.0	29.0	41.0
32	35.908997394047454	38.0	35.0	40.0	29.0	41.0
33	35.702285637544	38.0	34.0	40.0	27.0	41.0
34	35.56933205321629	38.0	34.0	40.0	27.0	41.0
35	35.33871096785992	38.0	34.0	40.0	26.0	41.0
36	35.190475986375894	38.0	34.0	40.0	25.0	41.0
37	35.03229723860467	38.0	33.0	40.0	25.0	41.0
38	34.96288374937137	38.0	33.0	40.0	25.0	41.0
39	34.86884372285466	38.0	33.0	40.0	24.0	41.0
40	34.76127388789832	38.0	33.0	40.0	24.0	41.0
41	34.56597603209436	38.0	33.0	40.0	23.0	41.0
42	34.355165329401544	38.0	33.0	40.0	22.0	41.0
43	34.0355989690486	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	4.0
15	9.0
16	15.0
17	31.0
18	65.0
19	125.0
20	361.0
21	658.0
22	1203.0
23	1985.0
24	3142.0
25	4768.0
26	6856.0
27	9408.0
28	12791.0
29	16476.0
30	20690.0
31	25454.0
32	31000.0
33	38456.0
34	46906.0
35	57164.0
36	72499.0
37	96330.0
38	125849.0
39	127689.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.85139784208842	20.911911946235083	13.120628171718558	24.11606203995794
2	19.06931490879166	23.276699583961964	34.59673455858822	23.05725094865816
3	19.66894116033466	24.911849083344766	31.90748868467974	23.51172107164084
4	14.167723906185708	16.37135395236136	36.067297581493165	33.39362455995977
5	13.003331733187034	38.32636126731587	34.86947378045993	13.80083321903717
6	32.955584510583826	37.16011178164861	13.871982581264572	16.012321126502997
7	26.835310656974354	32.28695194989256	21.71769990399122	19.160037489141864
8	27.379646139075575	34.235415809445435	19.053742056416585	19.331195995062405
9	25.080864536186166	14.490610570109267	21.13293215379692	39.29559273990765
10	16.48164975083436	27.404362684588307	33.4847757509258	22.629211813651533
11	33.708224751977326	22.54648996479678	23.00696063640104	20.73832464682485
12	21.160363233209896	26.709727746536828	30.924827412792027	21.205081607461253
13	31.34229415260824	20.992919352626526	25.639201298404423	22.02558519636081
14	21.080641658665936	22.491627805970833	27.089476752160195	29.338253783203033
15	23.267413020619028	29.3639704201527	24.657111507337813	22.71150505189046
16	22.654785580395924	27.99055913683537	25.893510263795548	23.461145018973166
17	22.312182828144287	26.650865221963148	26.698583870525304	24.338368079367257
18	23.26441274630823	25.674918849723404	28.50060576967037	22.560062634297992
19	23.16811822795227	25.190731724043342	29.397973529008365	22.243176518996023
20	23.97033443057651	24.88370365290541	29.176667581035982	21.9692943354821
21	23.169546930005026	25.606055410780414	29.427119050884652	21.797278608329904
22	22.27332213230924	25.864507612124537	28.993079367256435	22.86909088830979
23	22.92080990261967	25.984518584556305	29.632709276276685	21.46196223654734
24	22.746508252183055	26.145819046312806	28.105426781877203	23.002245919626937
25	22.96195652173913	26.0865279111233	28.693766287203402	22.257749279934167
26	22.679930736524483	26.689440177387645	28.741199195355005	21.889429890732867
27	21.99372514058428	26.868027933982535	28.46403099711974	22.674215928313444
28	22.287180542221	26.465562565720298	28.576469848671877	22.670787043386824
29	22.41347780368491	26.501280117039272	28.324303936359897	22.760938142915922
30	22.302753394596078	26.75958944817812	29.26624719974398	21.671409957481828
31	22.766652951126957	26.461562199972573	29.213242353586615	21.558542495313855
32	22.48577012755452	26.382269236044436	28.459316280345632	22.67264435605541
33	22.21117359301422	26.392698761029582	28.846637406848625	22.549490239107577
34	21.882572120879622	26.750017144424632	29.223957618982304	22.143453115713438
35	21.444103460887852	28.303016275773786	28.61561628491748	21.637263978420883
36	23.058965391121475	26.57485827275637	28.33959104832442	22.026585287797744
37	22.267607324098204	26.962893750285737	28.387881177707676	22.38161774790838
38	22.09573446715128	25.938514378457462	29.48383852237919	22.48191263201207
39	21.718271384812326	25.74321080784529	30.131754903305445	22.40676290403694
40	20.989490467699902	26.231826909888905	29.781865770584737	22.996816851826452
41	21.906574315365976	25.323315274539386	29.900019430347918	22.870090979746717
42	20.836905088465233	26.645721894573214	29.607706990353407	22.909666026608146
43	21.150933799661683	26.41827252777397	28.994365199103918	23.43642847346043
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	52.0
1	110.0
2	168.0
3	330.0
4	492.0
5	492.0
6	775.5
7	1059.0
8	1180.0
9	1301.0
10	1938.0
11	2575.0
12	2575.0
13	4736.5
14	6898.0
15	10433.5
16	13969.0
17	12983.5
18	11998.0
19	11998.0
20	12908.0
21	13818.0
22	11511.0
23	9204.0
24	10672.0
25	12140.0
26	12140.0
27	13516.0
28	14892.0
29	16404.5
30	17917.0
31	19450.0
32	20983.0
33	20983.0
34	23410.5
35	25838.0
36	27999.0
37	30160.0
38	32347.5
39	34535.0
40	34535.0
41	35704.0
42	36873.0
43	39012.5
44	41152.0
45	43173.5
46	45195.0
47	45195.0
48	55393.5
49	65592.0
50	63376.0
51	61160.0
52	57482.0
53	53804.0
54	53804.0
55	49274.0
56	44744.0
57	39296.5
58	33849.0
59	30399.0
60	26949.0
61	26949.0
62	24081.5
63	21214.0
64	18617.0
65	16020.0
66	13764.0
67	11508.0
68	11508.0
69	10004.5
70	8501.0
71	7166.5
72	5832.0
73	4831.5
74	3831.0
75	3831.0
76	3137.5
77	2444.0
78	1966.5
79	1489.0
80	1167.0
81	845.0
82	845.0
83	641.0
84	437.0
85	336.5
86	236.0
87	178.0
88	120.0
89	120.0
90	98.5
91	77.0
92	58.5
93	40.0
94	28.5
95	17.0
96	17.0
97	11.0
98	5.0
99	4.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	699936.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.130700971108475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.85599638425354	54.201492343108804
2	8.051634954166351	10.166107172400014
3	2.3162148387982646	4.386727991190525
4	1.0680946166788818	2.6971824741762087
5	0.6205754118029808	1.9588680376278242
6	0.4238080423647289	1.6053179275007146
7	0.2752343758516243	1.2163017355201002
8	0.21076410853597813	1.0644548729141672
9	0.16299209395720415	0.9260824629840365
>10	0.9159264634240499	10.586480562120117
>50	0.06319664123888254	2.7342643486543015
>100	0.030578849078138723	3.4884202667649333
>500	0.002718119917879913	1.0488855362064144
>1k	0.001812079945253275	2.146676164898527
>5k	4.5301998631331876E-4	1.7727381039333225
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6734	0.9620879623279843	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5663	0.8090739724774837	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4781	0.6830624514241301	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2624	0.3748914186439903	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	1488	0.2125908654505555	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	1443	0.2061617062131395	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1283	0.18330247336899372	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	1233	0.17615896310519819	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	1134	0.16201481278288302	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1026	0.1465848306130846	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	783	0.11186737073103828	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.11086727929410689	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.4287020527591095E-4	0.0
14	0.0	0.0	0.0	1.4287020527591095E-4	0.0
15	0.0	0.0	0.0	2.857404105518219E-4	0.0
16	0.0	0.0	0.0	8.572212316554657E-4	0.0
17	0.0	0.0	0.0	0.0015715722580350202	0.0
18	0.0	0.0	0.0	0.002143053079138664	0.0
19	0.0	0.0	0.0	0.0032860147213459516	0.0
20	0.0	0.0	0.0	0.005429067800484615	0.0
21	1.4287020527591095E-4	0.0	0.0	0.008857952727106478	2.857404105518219E-4
22	1.4287020527591095E-4	0.0	0.0	0.014287020527591093	2.857404105518219E-4
23	1.4287020527591095E-4	0.0	0.0	0.017144424633109313	2.857404105518219E-4
24	1.4287020527591095E-4	0.0	0.0	0.02300210304942166	2.857404105518219E-4
25	1.4287020527591095E-4	0.0	0.0	0.02700246879714717	2.857404105518219E-4
26	1.4287020527591095E-4	0.0	0.0	0.033717368445114984	2.857404105518219E-4
27	1.4287020527591095E-4	0.0	0.0	0.07643555982261235	2.857404105518219E-4
28	1.4287020527591095E-4	0.0	0.0	0.16001462990902024	2.857404105518219E-4
29	1.4287020527591095E-4	0.0	0.0	0.2674530242765053	2.857404105518219E-4
30	1.4287020527591095E-4	0.0	0.0	0.39789352169341197	2.857404105518219E-4
31	1.4287020527591095E-4	0.0	0.0	0.5717665615141956	2.857404105518219E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAAC	25	0.005494893	29.6	25
TGTTACG	50	2.7006396E-4	22.2	16
CGTGCGG	60	3.7236074E-5	21.583332	10
ACGAATG	45	0.0038243365	20.555555	18
GGTATCA	2290	0.0	20.034935	1
TCTAGAC	70	1.2183329E-4	18.5	3
CTGGTCG	60	9.2318986E-4	18.5	9
ACTAGGT	50	0.0070322007	18.5	3
GTCAATC	50	0.0070322007	18.5	16
ACCGTGT	50	0.0070322007	18.5	8
ATTGCGT	50	0.0070322007	18.5	6
ATACCGT	70	1.2183329E-4	18.5	6
TAGTACT	120	5.162292E-9	18.5	4
TTAACGG	75	2.0658108E-4	17.266666	35
TAGGAGC	75	2.0658108E-4	17.266666	6
CCGTTTA	110	7.79688E-7	16.818182	27
TACCCTC	90	4.441962E-5	16.444445	5
GTATTAG	160	6.2755134E-10	16.1875	1
TTATACC	80	3.3802004E-4	16.1875	4
ATTAGAG	115	1.2404198E-6	16.086956	3
>>END_MODULE