FastQCFastQC Report
Wed 25 May 2016
SRR2088435_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088435_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325774
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139471.051989253070282No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117650.8874061491626778No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75040.5660089879572234No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51490.38837690285071214No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG25610.19317017832601938No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG23000.17348356507217672No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22690.17114530832555172No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21060.15885060349652352No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA20340.15341981363339452No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT15300.11540428459149146No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG14550.10974721181739874No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13420.10122388883776572No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCTA450.00382611320.55555510
GGTATCA40250.020.4534151
TACGCAG609.2378375E-418.55
CGTAACG801.6169754E-518.526
GCGTAAC852.723626E-517.41176425
AGCGTAC752.067568E-417.26666610
TAGCGAA1107.809813E-716.81818218
TTCGCTA1353.9731822E-715.07407326
TCGTAGT750.00410484814.823
ACGAATG1001.0935645E-414.818
GTATCAA56900.014.5008782
GGCGTAA1152.2102386E-514.47826124
GTAATAC1551.2103737E-714.3225813
ATCTAGT1304.4449116E-614.2307691
GTTACAC1051.655933E-414.0952393
CTACTCG1601.7836828E-713.87514
GCCTATC800.006299293613.8757
TATACTG1601.7836828E-713.8755
ACGTTCG1203.3011107E-513.87499922
TGGCGTA1203.3011107E-513.87499923
Produced by FastQC (version 0.11.5)