##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088435_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1325774
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.798838263535114	31.0	31.0	31.0	28.0	34.0
2	30.989883645327183	31.0	31.0	34.0	28.0	34.0
3	31.06622093961716	31.0	31.0	34.0	28.0	34.0
4	34.83185897445568	35.0	35.0	37.0	32.0	37.0
5	34.625714488291365	35.0	35.0	37.0	32.0	37.0
6	34.77371708903629	35.0	35.0	37.0	32.0	37.0
7	34.62678857784208	35.0	35.0	37.0	31.0	37.0
8	34.68332159176451	35.0	35.0	37.0	31.0	37.0
9	36.148619598815486	38.0	35.0	39.0	32.0	39.0
10	35.87622626480833	37.0	35.0	39.0	30.0	39.0
11	35.9867254901665	37.0	35.0	39.0	30.0	39.0
12	35.77159229250234	37.0	35.0	39.0	30.0	39.0
13	35.83931499637193	37.0	35.0	39.0	30.0	39.0
14	36.65754495110026	38.0	36.0	40.0	31.0	41.0
15	36.69711051808227	38.0	36.0	40.0	31.0	41.0
16	36.51265751176294	38.0	35.0	40.0	30.0	41.0
17	36.581289872934605	38.0	35.0	40.0	30.0	41.0
18	36.43360557681777	38.0	35.0	40.0	30.0	41.0
19	36.51295545092904	38.0	35.0	40.0	30.0	41.0
20	36.39872029471086	38.0	35.0	40.0	30.0	41.0
21	36.22425617035784	38.0	34.0	40.0	29.0	41.0
22	36.10465282921523	38.0	34.0	40.0	29.0	41.0
23	35.88357593375643	38.0	34.0	40.0	27.0	41.0
24	36.108252990328666	38.0	34.0	40.0	29.0	41.0
25	36.06039113755436	38.0	34.0	40.0	29.0	41.0
26	35.830448477644005	38.0	34.0	40.0	28.0	41.0
27	35.754753826821165	38.0	34.0	40.0	27.0	41.0
28	35.5456533315633	38.0	34.0	40.0	27.0	41.0
29	35.37005552982635	38.0	34.0	40.0	27.0	41.0
30	35.210948472364066	38.0	34.0	40.0	26.0	41.0
31	34.92559214466417	38.0	33.0	40.0	25.0	41.0
32	34.768770544602624	37.0	33.0	40.0	25.0	41.0
33	34.555160985205625	37.0	33.0	40.0	25.0	41.0
34	34.42948194790364	37.0	33.0	40.0	24.0	41.0
35	34.145315868315414	37.0	33.0	40.0	24.0	41.0
36	34.020601550490504	37.0	33.0	40.0	23.0	41.0
37	33.865121053814605	37.0	32.0	40.0	23.0	41.0
38	33.78097549054364	37.0	32.0	40.0	22.0	41.0
39	33.690508337016716	37.0	32.0	40.0	21.0	41.0
40	33.563458779550665	37.0	32.0	40.0	21.0	41.0
41	33.348156623979655	37.0	31.0	40.0	19.0	41.0
42	33.122640812084114	37.0	31.0	40.0	18.0	41.0
43	32.7872744525085	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	7.0
14	12.0
15	21.0
16	62.0
17	148.0
18	323.0
19	674.0
20	1323.0
21	2397.0
22	4171.0
23	6526.0
24	10033.0
25	14360.0
26	19531.0
27	25472.0
28	33611.0
29	42717.0
30	52051.0
31	61795.0
32	74364.0
33	88359.0
34	105161.0
35	124426.0
36	153588.0
37	189580.0
38	218060.0
39	96998.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.743104028288386	24.37828770212721	14.722946746579735	22.155661523004675
2	21.554352400937113	25.395353959272093	31.871118305231512	21.179175334559282
3	22.005258814850798	25.993193410038213	31.222591482409523	20.77895629270147
4	16.727662482444217	18.844011121050798	34.25236880493961	30.17595759156538
5	12.7441026902021	39.9101958554022	34.431509442785874	12.91419201160982
6	32.49362259329267	38.74257603482947	13.499661329909923	15.264140041967938
7	26.184025331617605	33.04069924436593	21.78146501590769	18.99381040810877
8	27.180726126775756	35.39509750530633	18.88956941379149	18.53460695412642
9	25.192302760500656	14.196989833863086	20.760099383454495	39.85060802218176
10	16.17681444952156	28.721335612253675	33.70770583824996	21.394144099974806
11	34.342655686414126	22.663440375207237	22.548111518252732	20.445792420125905
12	20.536984433244278	27.765592023979956	30.92699057305393	20.77043296972184
13	31.49232071227826	21.159488721305443	25.730252667498384	21.617937898917916
14	20.59725111519761	22.766851665517652	27.538026843187453	29.097870376097283
15	23.355262661660284	29.968984155670576	24.582470315453463	22.09328286721568
16	22.174367576977673	28.380628975979317	26.31112089994222	23.133882547100786
17	22.140953133792035	27.426921933904268	27.172429086707083	23.25969584559661
18	22.7409799860308	26.18545845672038	28.857482497016836	22.216079060231987
19	22.88640447014348	25.679791578353473	29.831856711626564	21.60194723987648
20	23.45905109015564	25.51098452677455	29.58128610155275	21.44867828151706
21	22.734191498701893	26.141634999630405	29.784337300324186	21.339836201343516
22	21.995679504953333	26.5039139400833	29.440990696755254	22.059415858208112
23	22.5140182263342	26.55475216741315	29.891746255394963	21.039483350857687
24	22.52231526640287	26.804493073480096	28.270429198339986	22.402762461777044
25	22.62768767527497	26.528277066830398	29.003434974588426	21.840600283306205
26	22.250096924513528	27.29062419386713	29.04409047092491	21.415188410694434
27	22.00359940683706	26.900135317180755	28.834929633557454	22.261335642424726
28	22.021702039714157	27.078672533931126	29.11453988387161	21.785085542483106
29	22.367386900029718	26.751693727588567	28.662954621225033	22.217964751156682
30	22.16154487868973	26.95881801875735	29.627749525937304	21.251887576615623
31	22.54328414948551	26.906999232146656	29.482023331276675	21.067693287091164
32	22.10444615748989	26.61479256645552	28.898515131538254	22.382246144516333
33	22.039427534406318	26.80751017895961	29.140185280447493	22.012877006186574
34	21.776939357688416	27.0725628953351	29.543044289599884	21.607453457376597
35	21.32708893069256	28.444365329234095	28.99362938178	21.234916358293344
36	23.032281520078083	26.831269884610805	28.68505491886249	21.451393676448628
37	21.977727727350214	27.239484255989332	28.89293348640115	21.889854530259306
38	21.993492103480683	26.185232173809414	29.782904175221418	22.038371547488484
39	21.50057249576474	26.250854217988888	30.26511305848508	21.98346022776129
40	20.90839011777271	26.78601330241806	29.92847951460807	22.37711706520116
41	21.7976065302231	25.796628988047736	29.998853499917782	22.40691098181138
42	20.93758061328703	27.058759637766315	29.56816169271686	22.435498056229793
43	21.282586624869698	26.559881246728327	29.27587959938873	22.88165252901324
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	102.0
1	150.5
2	199.0
3	328.5
4	458.0
5	458.0
6	891.5
7	1325.0
8	1594.0
9	1863.0
10	3057.5
11	4252.0
12	4252.0
13	8623.5
14	12995.0
15	20925.0
16	28855.0
17	25547.0
18	22239.0
19	22239.0
20	25289.5
21	28340.0
22	23683.0
23	19026.0
24	21660.0
25	24294.0
26	24294.0
27	27197.0
28	30100.0
29	33744.5
30	37389.0
31	41470.5
32	45552.0
33	45552.0
34	50716.5
35	55881.0
36	60613.0
37	65345.0
38	68628.5
39	71912.0
40	71912.0
41	74305.5
42	76699.0
43	79980.5
44	83262.0
45	85601.0
46	87940.0
47	87940.0
48	102632.5
49	117325.0
50	111922.5
51	106520.0
52	99944.5
53	93369.0
54	93369.0
55	85405.5
56	77442.0
57	68195.0
58	58948.0
59	53307.0
60	47666.0
61	47666.0
62	42479.5
63	37293.0
64	32641.0
65	27989.0
66	24161.5
67	20334.0
68	20334.0
69	17478.0
70	14622.0
71	12336.0
72	10050.0
73	8381.0
74	6712.0
75	6712.0
76	5400.0
77	4088.0
78	3224.0
79	2360.0
80	1883.5
81	1407.0
82	1407.0
83	1093.0
84	779.0
85	592.0
86	405.0
87	315.0
88	225.0
89	225.0
90	180.0
91	135.0
92	90.5
93	46.0
94	32.0
95	18.0
96	18.0
97	14.0
98	10.0
99	6.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1325774.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.431392553183215
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.93746877649377	53.65195848212208
2	8.53337944766213	10.655015242045202
3	2.264100017373372	4.240527508929177
4	0.9443041913127219	2.3581690262984307
5	0.5253050865235579	1.6397764033468056
6	0.3501602217278497	1.3116594155520638
7	0.22802951774821933	0.9965340235376499
8	0.16432345653086072	0.8207153776299283
9	0.13140408920114405	0.7383366248409114
>10	0.7992073404784167	9.3132726660463
>50	0.0729076186332251	3.2198472148162205
>100	0.04432390714171322	5.39543176005561
>500	0.0035119891910373683	1.4979127385890019
>1k	0.0010899276799771142	1.2602064166566467
>5k	2.4220615110602536E-4	0.956646975639342
>10k+	2.4220615110602536E-4	1.9439901238946302
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13947	1.051989253070282	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11765	0.8874061491626778	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7504	0.5660089879572234	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5149	0.38837690285071214	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2561	0.19317017832601938	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2300	0.17348356507217672	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2269	0.17114530832555172	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2106	0.15885060349652352	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2034	0.15341981363339452	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1530	0.11540428459149146	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1455	0.10974721181739874	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1342	0.10122388883776572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.542763698790292E-5
2	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
3	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
4	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
5	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
6	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
7	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
8	0.0	0.0	0.0	7.542763698790292E-5	7.542763698790292E-5
9	0.0	0.0	0.0	1.5085527397580583E-4	7.542763698790292E-5
10	0.0	0.0	0.0	1.5085527397580583E-4	7.542763698790292E-5
11	0.0	0.0	0.0	2.2628291096370875E-4	7.542763698790292E-5
12	0.0	0.0	0.0	4.525658219274175E-4	7.542763698790292E-5
13	0.0	0.0	0.0	6.034210959032233E-4	7.542763698790292E-5
14	0.0	0.0	0.0	6.034210959032233E-4	7.542763698790292E-5
15	0.0	0.0	0.0	6.788487328911263E-4	7.542763698790292E-5
16	0.0	0.0	0.0	0.0012068421918064467	7.542763698790292E-5
17	0.0	0.0	0.0	0.0015085527397580582	7.542763698790292E-5
18	0.0	0.0	0.0	0.0017348356507217672	7.542763698790292E-5
19	0.0	0.0	0.0	0.0021874014726491844	7.542763698790292E-5
20	0.0	0.0	0.0	0.00362052657541934	7.542763698790292E-5
21	0.0	0.0	0.0	0.006185066233008039	7.542763698790292E-5
22	0.0	0.0	0.0	0.010710724452282215	7.542763698790292E-5
23	0.0	0.0	0.0	0.012822698287943496	7.542763698790292E-5
24	0.0	0.0	0.0	0.016895790685290252	7.542763698790292E-5
25	0.0	0.0	0.0	0.020516317260709592	7.542763698790292E-5
26	0.0	0.0	0.0	0.02662595585672973	7.542763698790292E-5
27	0.0	0.0	0.0	0.05770214229574573	7.542763698790292E-5
28	0.0	0.0	0.0	0.12551158794787046	7.542763698790292E-5
29	0.0	0.0	0.0	0.2123287981209467	7.542763698790292E-5
30	0.0	0.0	0.0	0.3207937401095511	7.542763698790292E-5
31	0.0	0.0	0.0	0.45399894703018767	7.542763698790292E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGCTA	45	0.003826113	20.555555	10
GGTATCA	4025	0.0	20.453415	1
TACGCAG	60	9.2378375E-4	18.5	5
CGTAACG	80	1.6169754E-5	18.5	26
GCGTAAC	85	2.723626E-5	17.411764	25
AGCGTAC	75	2.067568E-4	17.266666	10
TAGCGAA	110	7.809813E-7	16.818182	18
TTCGCTA	135	3.9731822E-7	15.074073	26
TCGTAGT	75	0.004104848	14.8	23
ACGAATG	100	1.0935645E-4	14.8	18
GTATCAA	5690	0.0	14.500878	2
GGCGTAA	115	2.2102386E-5	14.478261	24
GTAATAC	155	1.2103737E-7	14.322581	3
ATCTAGT	130	4.4449116E-6	14.230769	1
GTTACAC	105	1.655933E-4	14.095239	3
CTACTCG	160	1.7836828E-7	13.875	14
GCCTATC	80	0.0062992936	13.875	7
TATACTG	160	1.7836828E-7	13.875	5
ACGTTCG	120	3.3011107E-5	13.874999	22
TGGCGTA	120	3.3011107E-5	13.874999	23
>>END_MODULE