FastQCFastQC Report
Wed 25 May 2016
SRR2088434_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088434_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3088815
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT476011.5410764322240083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT384201.2438427034315749No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT328661.0640326468241057No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166630.5394625446975619No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG104440.338123196112425No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA102670.3323928432100984No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG95370.30875918434739535No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82110.2658300998926773No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA80530.260714869618284No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG75570.24465693154170776No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57070.18476341250609055No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT51970.1682522261773528No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA49560.16044988126514537No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44870.14526606481773754No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42880.1388234646620144No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38340.12412527134192239No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38180.12360727333945219No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG37960.12289502608605565No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36980.11972228832092566No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36130.11697042393280271No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35160.1138300610428271No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG34920.1130530640391218No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC31590.1022722306127107No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA149250.019.6837521
GTATTGG15500.018.977421
TTGGACC22250.016.7955064
ATTGGAC18350.016.7356953
ACCGTTA5550.016.6666668
ACGTTTA1059.353231E-615.85714226
ATAACGC1302.5928966E-715.6538463
TGGACCC23550.015.554145
GGACCCT23750.014.9557896
GTATTAG5700.014.9298261
TACATCG1252.9624753E-614.8000015
CTCTAAT4800.014.6458331
CCGTTAT6550.014.4045799
CTAATAC5450.014.2568813
ACCGTGT2353.6379788E-1214.1702148
CGTTTAC6550.014.12213728
TATACTG3050.013.950825
TATTGGA20050.013.9326692
CGAACTA5050.013.92079224
AGAGTCG1203.3039054E-513.87499922
Produced by FastQC (version 0.11.5)