##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088434_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3088815
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385456882331898	31.0	31.0	34.0	30.0	34.0
2	31.563425132291833	31.0	31.0	34.0	30.0	34.0
3	31.675313672071653	31.0	31.0	34.0	30.0	34.0
4	35.3663430797895	37.0	35.0	37.0	33.0	37.0
5	35.25976887576627	37.0	35.0	37.0	33.0	37.0
6	35.32437293913685	37.0	35.0	37.0	33.0	37.0
7	35.14072710732109	37.0	35.0	37.0	32.0	37.0
8	35.20031144629898	37.0	35.0	37.0	32.0	37.0
9	36.72424764837	39.0	35.0	39.0	32.0	39.0
10	36.585846028331254	39.0	35.0	39.0	32.0	39.0
11	36.617984243148264	39.0	35.0	39.0	32.0	39.0
12	36.464245997251375	38.0	35.0	39.0	32.0	39.0
13	36.47924916189542	38.0	35.0	39.0	32.0	39.0
14	37.3761037808998	39.0	36.0	41.0	32.0	41.0
15	37.40990994928476	39.0	36.0	41.0	32.0	41.0
16	37.269133632153434	39.0	36.0	41.0	32.0	41.0
17	37.37091926839257	39.0	36.0	41.0	32.0	41.0
18	37.25880280949167	39.0	36.0	40.0	31.0	41.0
19	37.34655814608515	39.0	36.0	41.0	31.0	41.0
20	37.25802807872922	39.0	36.0	41.0	31.0	41.0
21	37.101973086766286	39.0	36.0	40.0	31.0	41.0
22	37.007617160626324	39.0	36.0	40.0	31.0	41.0
23	36.79198009592675	39.0	35.0	40.0	30.0	41.0
24	36.982840992419426	39.0	36.0	40.0	31.0	41.0
25	36.94924105198919	39.0	36.0	41.0	31.0	41.0
26	36.74789652342403	39.0	35.0	40.0	30.0	41.0
27	36.68856406097484	39.0	35.0	40.0	30.0	41.0
28	36.47097673379597	39.0	35.0	40.0	30.0	41.0
29	36.28545413046751	38.0	35.0	40.0	30.0	41.0
30	36.07948452723779	38.0	35.0	40.0	29.0	41.0
31	35.762809362166394	38.0	34.0	40.0	28.0	41.0
32	35.598377695006015	38.0	34.0	40.0	27.0	41.0
33	35.366788881820376	38.0	34.0	40.0	27.0	41.0
34	35.219853568439675	38.0	34.0	40.0	26.0	41.0
35	34.94918212971641	38.0	33.0	40.0	25.0	41.0
36	34.77670789607018	38.0	33.0	40.0	25.0	41.0
37	34.58762276147973	38.0	33.0	40.0	24.0	41.0
38	34.47048593068863	38.0	33.0	40.0	24.0	41.0
39	34.34138269854297	38.0	33.0	40.0	23.0	41.0
40	34.170607174596086	37.0	33.0	40.0	22.0	41.0
41	33.92380087509287	37.0	33.0	40.0	21.0	41.0
42	33.62413385068384	37.0	32.0	40.0	19.0	41.0
43	33.26210763674742	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	1.0
13	4.0
14	11.0
15	25.0
16	60.0
17	139.0
18	304.0
19	713.0
20	1588.0
21	3217.0
22	5966.0
23	10046.0
24	16175.0
25	23902.0
26	34769.0
27	47491.0
28	62447.0
29	80986.0
30	100137.0
31	120443.0
32	145829.0
33	177828.0
34	213633.0
35	257471.0
36	323607.0
37	434411.0
38	525069.0
39	502538.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.23953198880477	21.03123689829271	14.04224597458896	22.68698513831356
2	19.2758064176715	23.955756495614015	34.519840132866484	22.248596953847997
3	20.245013055168407	24.835122854557493	32.65362282946697	22.26624126080714
4	13.853403327813417	16.185559834434887	35.73758868692363	34.22344815082807
5	12.648475224317416	38.78166869819008	34.354663519828804	14.2151925576637
6	32.661813672881024	36.88356214276349	12.904884235540168	17.549739948815322
7	26.15167952758582	33.02111003734442	20.881762099704904	19.94544833536486
8	28.146036586846414	33.572680785349725	18.268365052617266	20.012917575186602
9	24.625333663557058	14.419445645012733	21.62978359014703	39.32543710128318
10	17.087491481360974	27.468041951363226	32.40404491690179	23.04042165037401
11	33.133612728505916	22.44349370227741	23.204497517656446	21.218396051560227
12	21.914650116630487	27.385712643845615	30.30145865000008	20.398178589523813
13	32.78231295820566	20.52683634338735	25.760655785471126	20.930194912935868
14	20.752424473463122	23.16807578310776	27.721245849945692	28.358253893483422
15	23.124790575026346	29.245649221465193	25.520045713323718	22.109514490184747
16	21.93961114537452	27.971697884140035	26.5554913453865	23.53319962509895
17	22.223571175353655	26.24757390779312	26.75605369696793	24.772801219885295
18	23.653990284299965	24.70235349154935	29.461654388495262	22.18200183565542
19	23.068361167632247	24.231137183677234	30.917584899063232	21.782916749627283
20	24.186945479091495	23.7335353525543	30.517075318528303	21.562443849825904
21	22.718518266713932	24.887149278930593	31.196105950016427	21.198226504339043
22	21.419541150894435	25.39093471120802	30.64502082513844	22.5445033127591
23	22.503128222311794	25.52163208220628	31.489098570163637	20.486141125318287
24	22.42895738333309	25.69043468126126	28.958969701973086	22.92163823343256
25	22.353977172475528	25.692150549644445	29.984443872488313	21.96942840539171
26	22.124180308629686	26.623575707836178	30.201808784274874	21.05043519925926
27	21.08650081018125	26.51408388006404	29.80864830039999	22.59076700935472
28	21.437217832728734	26.43868279582947	29.79084211906508	22.333257252376722
29	21.936179408608155	26.229476352581816	29.113041732832816	22.72130250597721
30	21.53068409729945	26.523925842110973	31.265873805974138	20.679516254615443
31	22.546445805268363	26.16514747565005	30.75716739267324	20.53123932640835
32	21.99856579303066	25.95221792175964	29.548710427785412	22.500505857424287
33	21.592390609343713	26.151970901462214	30.22074808624019	22.034890402953884
34	20.960206422203985	26.867941265501493	30.65687002944495	21.51498228284957
35	20.282114662095335	29.238235375054835	29.517468673261426	20.962181289588404
36	22.89933194445119	26.628108190357793	29.39259877979096	21.079961085400065
37	21.578145664275784	27.516021516342025	29.23334676890652	21.672486050475666
38	21.47438418940597	25.819221934625414	30.812463679436934	21.89393019653168
39	20.974516117022223	25.46915240958102	31.55533756472952	22.00099390866724
40	19.69357180666372	26.44421889948087	31.216210747487306	22.645998546368105
41	21.51274841646392	24.982946534512426	31.201674428542987	22.30263062048067
42	19.7723722527895	27.188355404904467	30.797700736366533	22.241571605939495
43	20.3335259638405	26.989832670457766	29.801137329364174	22.87550403633756
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	166.0
1	365.5
2	565.0
3	1596.0
4	2627.0
5	2627.0
6	4133.0
7	5639.0
8	6272.0
9	6905.0
10	10772.5
11	14640.0
12	14640.0
13	28154.5
14	41669.0
15	67630.0
16	93591.0
17	84289.5
18	74988.0
19	74988.0
20	77663.5
21	80339.0
22	61333.0
23	42327.0
24	46284.5
25	50242.0
26	50242.0
27	54200.5
28	58159.0
29	62296.5
30	66434.0
31	71816.0
32	77198.0
33	77198.0
34	85758.0
35	94318.0
36	105022.0
37	115726.0
38	123578.5
39	131431.0
40	131431.0
41	136853.5
42	142276.0
43	154003.0
44	165730.0
45	181251.5
46	196773.0
47	196773.0
48	267553.0
49	338333.0
50	322286.0
51	306239.0
52	281626.0
53	257013.0
54	257013.0
55	228745.5
56	200478.0
57	168789.5
58	137101.0
59	121315.0
60	105529.0
61	105529.0
62	93613.0
63	81697.0
64	71925.5
65	62154.0
66	53972.5
67	45791.0
68	45791.0
69	39688.0
70	33585.0
71	28588.5
72	23592.0
73	19497.5
74	15403.0
75	15403.0
76	12268.0
77	9133.0
78	7144.5
79	5156.0
80	3999.0
81	2842.0
82	2842.0
83	2159.0
84	1476.0
85	1109.0
86	742.0
87	578.0
88	414.0
89	414.0
90	334.0
91	254.0
92	178.0
93	102.0
94	62.0
95	22.0
96	22.0
97	16.5
98	11.0
99	8.0
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3088815.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.128111607106725
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16136935203755	33.70952260778966
2	11.7300881502915	10.117931018139439
3	4.090368853059173	5.292296532269073
4	1.782714252156724	3.0754039692237995
5	0.9858523655850296	2.1258975425540667
6	0.5800650152284629	1.5010265229690725
7	0.42599007222722224	1.2860503164974568
8	0.2870985034405203	0.9905613038892859
9	0.21873274660133463	0.8490177276796216
>10	1.4464430179266294	12.030547840168234
>50	0.15795109945602498	4.737124813315254
>100	0.11681289233525542	9.857159068524838
>500	0.0095010211711314	2.7538872882442953
>1k	0.006107799324289179	5.1524206435027855
>5k	4.5242957957697627E-4	1.439432212091522
>10k+	4.5242957957697627E-4	5.081720593141596
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	47601	1.5410764322240083	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38420	1.2438427034315749	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32866	1.0640326468241057	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16663	0.5394625446975619	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10444	0.338123196112425	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10267	0.3323928432100984	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9537	0.30875918434739535	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8211	0.2658300998926773	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8053	0.260714869618284	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7557	0.24465693154170776	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5707	0.18476341250609055	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5197	0.1682522261773528	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4956	0.16044988126514537	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4487	0.14526606481773754	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4288	0.1388234646620144	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3834	0.12412527134192239	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3818	0.12360727333945219	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3796	0.12289502608605565	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3698	0.11972228832092566	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3613	0.11697042393280271	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3516	0.1138300610428271	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3492	0.1130530640391218	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3159	0.1022722306127107	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	6.474975030877537E-5	0.0	0.0	0.0	0.0
7	6.474975030877537E-5	0.0	0.0	0.0	0.0
8	9.712462546316306E-5	0.0	0.0	0.0	0.0
9	9.712462546316306E-5	0.0	0.0	1.9424925092632612E-4	0.0
10	1.6187437577193842E-4	0.0	0.0	2.913738763894892E-4	0.0
11	1.6187437577193842E-4	0.0	0.0	5.17998002470203E-4	0.0
12	1.6187437577193842E-4	0.0	0.0	6.474975030877537E-4	0.0
13	1.6187437577193842E-4	0.0	0.0	6.798723782421414E-4	0.0
14	1.9424925092632612E-4	0.0	0.0	9.064965043228552E-4	0.0
15	1.9424925092632612E-4	0.0	0.0	0.0012302452558667321	0.0
16	2.266241260807138E-4	0.0	0.0	0.0015216191322562213	0.0
17	2.266241260807138E-4	0.0	0.0	0.002395740761424689	0.0
18	2.266241260807138E-4	0.0	0.0	0.002784239263277341	0.0
19	2.589990012351015E-4	0.0	0.0	0.0039821096439896855	0.0
20	2.589990012351015E-4	0.0	0.0	0.00699297303334774	0.0
21	2.589990012351015E-4	0.0	0.0	0.011719704805888343	0.0
22	2.913738763894892E-4	0.0	0.0	0.018000430585839552	0.0
23	2.913738763894892E-4	0.0	0.0	0.02240341360683628	0.0
24	2.913738763894892E-4	0.0	0.0	0.028781264012250653	0.0
25	2.913738763894892E-4	0.0	0.0	0.033410871159328094	0.0
26	3.2374875154387683E-4	0.0	0.0	0.042993834205026844	0.0
27	3.2374875154387683E-4	0.0	0.0	0.09663900233584724	0.0
28	3.2374875154387683E-4	0.0	0.0	0.21396554989534822	0.0
29	3.2374875154387683E-4	0.0	0.0	0.3628576007303772	0.0
30	3.2374875154387683E-4	0.0	0.0	0.564261699065823	0.0
31	3.2374875154387683E-4	0.0	0.0	0.7913714482738526	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	14925	0.0	19.683752	1
GTATTGG	1550	0.0	18.97742	1
TTGGACC	2225	0.0	16.795506	4
ATTGGAC	1835	0.0	16.735695	3
ACCGTTA	555	0.0	16.666666	8
ACGTTTA	105	9.353231E-6	15.857142	26
ATAACGC	130	2.5928966E-7	15.653846	3
TGGACCC	2355	0.0	15.55414	5
GGACCCT	2375	0.0	14.955789	6
GTATTAG	570	0.0	14.929826	1
TACATCG	125	2.9624753E-6	14.800001	5
CTCTAAT	480	0.0	14.645833	1
CCGTTAT	655	0.0	14.404579	9
CTAATAC	545	0.0	14.256881	3
ACCGTGT	235	3.6379788E-12	14.170214	8
CGTTTAC	655	0.0	14.122137	28
TATACTG	305	0.0	13.95082	5
TATTGGA	2005	0.0	13.932669	2
CGAACTA	505	0.0	13.920792	24
AGAGTCG	120	3.3039054E-5	13.874999	22
>>END_MODULE