FastQCFastQC Report
Wed 25 May 2016
SRR2088433_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088433_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438419
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440321.8057602077411634No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT366321.5022848821305936No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT289641.1878188285114248No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156660.6424654663534036No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG113700.4662857367827268No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA99460.40788724169226037No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG93890.3850445719131946No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92080.3776217294894766No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA82530.33845700841405846No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG72960.29921026698036723No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA53360.21883031587270277No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49580.2033284681590818No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC43130.1768769026159983No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41200.16896193804264156No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA40460.16592718478653587No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG40250.16506597102466802No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38460.15772514895922318No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38160.15649484358512628No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC38120.15633080286858No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35900.14722654310026292No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35700.14640633951753165No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG35090.14390471859020126No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA30150.12364569009673891No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30040.12319457812623671No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29410.1206109368406332No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT29320.12024184522840414No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT26150.10724161844211351No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA24890.10207433587090653No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24720.10137716282558494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121150.022.7985951
GATCGGT502.7033064E-422.211
TACGTTA902.1542328E-618.519
AATACTG5000.017.395
GTATTGG14500.017.3517231
CAGATCG1201.04259016E-716.9583329
GTATACT1554.0199666E-1016.7096794
ACGTTTA904.449249E-516.44444526
ACGTTAA1059.3516155E-615.85714220
TGGACCC22300.015.5964135
TTGGACC22100.015.5701364
CTCTAAT4100.015.3414631
CCGAGTT2300.015.28260913
CCGTTTA3900.015.17948727
ATTGGAC16750.015.1313433
GTATCAA184450.014.9945782
TACTGGT4700.014.9574477
ACCGTTA4150.014.7108438
CTAGGCA3400.014.6911754
TGTTACG2400.014.64583316
Produced by FastQC (version 0.11.5)