##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088433_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2438419
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.170054859316632	31.0	31.0	33.0	30.0	34.0
2	31.347646159253188	31.0	31.0	34.0	30.0	34.0
3	31.441474578405106	31.0	31.0	34.0	28.0	34.0
4	35.1475903033892	35.0	35.0	37.0	33.0	37.0
5	35.04183366353362	35.0	35.0	37.0	32.0	37.0
6	35.135154786769625	37.0	35.0	37.0	32.0	37.0
7	34.93351962890709	36.0	35.0	37.0	32.0	37.0
8	35.02249572366357	36.0	35.0	37.0	32.0	37.0
9	36.48819952600435	38.0	35.0	39.0	32.0	39.0
10	36.347653541085435	38.0	35.0	39.0	32.0	39.0
11	36.38615635786959	38.0	35.0	39.0	32.0	39.0
12	36.22372077973474	38.0	35.0	39.0	32.0	39.0
13	36.26312376995094	38.0	35.0	39.0	32.0	39.0
14	37.08767115085635	39.0	36.0	40.0	31.0	41.0
15	37.13297345534135	39.0	36.0	40.0	31.0	41.0
16	36.96135733850499	39.0	36.0	40.0	31.0	41.0
17	37.10726089322631	39.0	36.0	40.0	31.0	41.0
18	36.98224136212849	39.0	36.0	40.0	31.0	41.0
19	37.09212362600521	39.0	36.0	40.0	31.0	41.0
20	36.99996432114415	39.0	36.0	40.0	31.0	41.0
21	36.844076838312034	39.0	36.0	40.0	30.0	41.0
22	36.75506506469971	39.0	35.0	40.0	30.0	41.0
23	36.529233901146604	38.0	35.0	40.0	30.0	41.0
24	36.71411968164618	39.0	35.0	40.0	30.0	41.0
25	36.679035473394855	39.0	35.0	40.0	30.0	41.0
26	36.45029299722484	38.0	35.0	40.0	30.0	41.0
27	36.38535624927463	38.0	35.0	40.0	30.0	41.0
28	36.16058027763071	38.0	35.0	40.0	30.0	41.0
29	35.962615120699105	38.0	35.0	40.0	29.0	41.0
30	35.749727999986874	38.0	34.0	40.0	28.0	41.0
31	35.39005888651622	38.0	34.0	40.0	27.0	41.0
32	35.23893760670336	38.0	34.0	40.0	27.0	41.0
33	34.983101345584984	38.0	33.0	40.0	25.0	41.0
34	34.83257430326781	37.0	33.0	40.0	25.0	41.0
35	34.53820036671302	37.0	33.0	40.0	24.0	41.0
36	34.36698984054832	37.0	33.0	40.0	24.0	41.0
37	34.16988343676784	37.0	33.0	40.0	23.0	41.0
38	34.03091634374568	37.0	33.0	40.0	22.0	41.0
39	33.88501360922795	37.0	33.0	40.0	21.0	41.0
40	33.684649356816855	37.0	33.0	40.0	20.0	41.0
41	33.40542622084227	37.0	32.0	40.0	18.0	41.0
42	33.0892459417352	37.0	31.0	40.0	16.0	41.0
43	32.72559432976859	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	3.0
13	3.0
14	8.0
15	27.0
16	49.0
17	133.0
18	356.0
19	760.0
20	1530.0
21	3105.0
22	5632.0
23	9291.0
24	14959.0
25	22121.0
26	31325.0
27	42786.0
28	56145.0
29	70603.0
30	87514.0
31	103541.0
32	124861.0
33	149571.0
34	176627.0
35	210778.0
36	264776.0
37	345741.0
38	407166.0
39	309007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.97560755555136	22.3954127654025	15.03962198457279	21.589357694473346
2	19.50312887161722	25.019982209784292	34.31551345359431	21.161375465004166
3	20.593753575574993	25.19665406150461	33.58438397994766	20.625208382972737
4	14.20965797920702	16.8383694516816	35.68316191761957	33.26881065149181
5	11.916983914577438	39.88309638335331	34.42927569051915	13.770644011550107
6	31.94414085520167	37.3530554018813	12.68059345010025	18.02221029281678
7	25.127551909659495	34.0630137806505	20.89562950419924	19.91380480549077
8	27.938799689470923	34.05173598138794	18.36796711311715	19.64149721602399
9	24.050788646249885	14.509975521024074	22.468779975877812	38.97045585684823
10	16.75630808322934	28.105629098198463	32.620029617551374	22.518033201020828
11	32.321065411645826	22.426498481188016	24.104429960560513	21.14800614660565
12	21.225269324098935	28.064413868166216	30.9475114818249	19.762805325909945
13	32.75671654461354	20.662117544195645	26.91604683198417	19.66511907920665
14	20.04483232783209	23.672264692819407	28.52249756912163	27.760405410226873
15	21.934376331549256	29.948585538416488	26.58484862527728	21.53218950475698
16	20.755128630477373	28.700030634603813	27.499621681097462	23.04521905382135
17	21.417730094786826	26.458291212461845	27.47358021734575	24.65039847540558
18	23.141346913717452	24.79524642811592	30.642600799944553	21.42080585822207
19	22.336809219416352	23.92049930713302	32.36182952970757	21.380861943743056
20	23.636954928582824	23.607878711575	32.193154662918886	20.562011696923292
21	22.299038844431575	24.705024034015484	33.02537422813717	19.970562893415774
22	20.301802110301796	25.229052103022493	32.46279659074179	22.00634919593392
23	21.85887659175884	25.60774009716952	33.54739279836648	18.985990512705158
24	21.60309610448409	25.52596579997121	30.391577493449653	22.479360602095046
25	21.462144118791727	26.063158136481057	31.636482491319168	20.83821525340805
26	21.40407370513435	26.765826545806938	31.66313910775794	20.166960641300776
27	19.788108606437206	26.94155516340711	31.403011541494713	21.867324688660972
28	20.5929743821714	26.439098448625938	31.354865591188393	21.61306157801428
29	20.8994024406798	26.137304540359963	30.608603361440345	22.354689657519895
30	20.803684682575064	26.51558243271562	32.78050244851274	19.900230436196566
31	21.870769543708445	26.180816340423856	32.34780404844286	19.600610067424835
32	21.403212491372482	26.02120472322435	30.935659540054438	21.639923245348726
33	20.913427921944503	26.224287130308614	31.621021653784688	21.241263293962195
34	19.874722104773625	27.321186391674278	32.11503027166373	20.689061231888367
35	19.32653904025518	30.055663116142057	30.689147353264556	19.928650490338207
36	22.253312494694306	26.600186432274352	30.785685314952023	20.360815758079312
37	21.096128269997898	27.73395384468379	30.524204412777294	20.64571347254102
38	20.830915441521743	25.734625591418048	32.207959337587184	21.226499629473032
39	20.303688578542083	25.53014883824314	33.09718305180529	21.06897953140949
40	18.772081418328842	26.61052099741677	32.32512541938035	22.292272164874046
41	20.945087780237934	25.250582447069185	32.29231727607109	21.512012496621786
42	19.25460718604965	27.766720977813904	31.57074317416326	21.407928661973187
43	19.588676105296095	27.843738094232368	30.387066373744627	22.180519426726907
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	160.0
1	333.0
2	506.0
3	1141.0
4	1776.0
5	1776.0
6	3156.5
7	4537.0
8	5253.5
9	5970.0
10	9187.0
11	12404.0
12	12404.0
13	24977.0
14	37550.0
15	61483.5
16	85417.0
17	76418.5
18	67420.0
19	67420.0
20	71413.5
21	75407.0
22	57331.5
23	39256.0
24	42073.5
25	44891.0
26	44891.0
27	47936.0
28	50981.0
29	54028.0
30	57075.0
31	61733.5
32	66392.0
33	66392.0
34	72738.5
35	79085.0
36	86031.5
37	92978.0
38	98041.0
39	103104.0
40	103104.0
41	107917.0
42	112730.0
43	120773.0
44	128816.0
45	143591.0
46	158366.0
47	158366.0
48	225874.5
49	293383.0
50	271201.5
51	249020.0
52	226257.5
53	203495.0
54	203495.0
55	174799.0
56	146103.0
57	119645.0
58	93187.0
59	80387.0
60	67587.0
61	67587.0
62	58603.0
63	49619.0
64	42678.0
65	35737.0
66	30696.0
67	25655.0
68	25655.0
69	22028.0
70	18401.0
71	15498.0
72	12595.0
73	10349.0
74	8103.0
75	8103.0
76	6483.5
77	4864.0
78	3809.5
79	2755.0
80	2116.5
81	1478.0
82	1478.0
83	1151.0
84	824.0
85	612.0
86	400.0
87	296.0
88	192.0
89	192.0
90	160.0
91	128.0
92	87.5
93	47.0
94	28.5
95	10.0
96	10.0
97	9.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2438419.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.01059451928652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.29960700438544	35.340334439280674
2	10.897289135998744	9.591923470477338
3	3.449595744202874	4.5545627866070735
4	1.4954559161811247	2.6326361579406243
5	0.819526879153195	1.8033932588033794
6	0.503354985618044	1.3291771302778224
7	0.34913448792406593	1.0755931466507362
8	0.2636724024621036	0.9283503352548614
9	0.21225475156852394	0.8407312025466782
>10	1.4278382170309427	12.347075551357648
>50	0.15739696456136446	4.786481058594391
>100	0.11080328983350679	9.337608893158441
>500	0.006941309066109928	2.1122648114666607
>1k	0.0060970958013127725	5.637508704785906
>5k	5.628088431981021E-4	2.0405156764486976
>10k+	4.690073693317517E-4	5.641843376349131
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	44032	1.8057602077411634	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	36632	1.5022848821305936	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28964	1.1878188285114248	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15666	0.6424654663534036	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11370	0.4662857367827268	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9946	0.40788724169226037	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9389	0.3850445719131946	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9208	0.3776217294894766	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	8253	0.33845700841405846	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7296	0.29921026698036723	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5336	0.21883031587270277	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4958	0.2033284681590818	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4313	0.1768769026159983	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4120	0.16896193804264156	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4046	0.16592718478653587	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4025	0.16506597102466802	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3846	0.15772514895922318	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3816	0.15649484358512628	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3812	0.15633080286858	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3590	0.14722654310026292	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3570	0.14640633951753165	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3509	0.14390471859020126	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3015	0.12364569009673891	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3004	0.12319457812623671	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	2941	0.1206109368406332	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2932	0.12024184522840414	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2615	0.10724161844211351	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	2489	0.10207433587090653	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2472	0.10137716282558494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	4.101017913656349E-5
7	0.0	0.0	0.0	0.0	4.101017913656349E-5
8	4.101017913656349E-5	0.0	0.0	0.0	4.101017913656349E-5
9	4.101017913656349E-5	0.0	0.0	8.202035827312698E-5	4.101017913656349E-5
10	8.202035827312698E-5	0.0	0.0	1.6404071654625395E-4	4.101017913656349E-5
11	2.0505089568281743E-4	0.0	0.0	4.1010179136563487E-4	4.101017913656349E-5
12	2.0505089568281743E-4	0.0	0.0	7.791934035947062E-4	4.101017913656349E-5
13	2.4606107481938094E-4	0.0	0.0	8.612137618678332E-4	4.101017913656349E-5
14	2.4606107481938094E-4	0.0	0.0	0.0011482850158237776	4.101017913656349E-5
15	2.870712539559444E-4	0.0	0.0	0.001435356269779722	4.101017913656349E-5
16	2.870712539559444E-4	0.0	0.0	0.0022145496733744284	4.101017913656349E-5
17	2.870712539559444E-4	0.0	0.0	0.0034858652266078964	4.101017913656349E-5
18	2.870712539559444E-4	0.0	0.0	0.004018997555383222	4.101017913656349E-5
19	2.870712539559444E-4	0.0	0.0	0.005618394541709198	4.101017913656349E-5
20	3.280814330925079E-4	0.0	0.0	0.009104259768317094	4.101017913656349E-5
21	3.280814330925079E-4	0.0	0.0	0.016650132729444777	4.101017913656349E-5
22	3.6909161222907136E-4	0.0	0.0	0.02739479966322441	4.101017913656349E-5
23	3.6909161222907136E-4	0.0	0.0	0.03457158101212302	4.101017913656349E-5
24	4.921221496387619E-4	0.0	0.0	0.04527523776676609	4.101017913656349E-5
25	4.921221496387619E-4	0.0	4.101017913656349E-5	0.05187787660775281	4.101017913656349E-5
26	4.921221496387619E-4	0.0	4.101017913656349E-5	0.06217143157103024	4.101017913656349E-5
27	4.921221496387619E-4	0.0	4.101017913656349E-5	0.1123268806550474	4.101017913656349E-5
28	4.921221496387619E-4	0.0	4.101017913656349E-5	0.21940445838061465	4.101017913656349E-5
29	4.921221496387619E-4	0.0	4.101017913656349E-5	0.35970028120679837	4.101017913656349E-5
30	5.741425079118888E-4	0.0	4.101017913656349E-5	0.5647921870687523	4.101017913656349E-5
31	5.741425079118888E-4	0.0	4.101017913656349E-5	0.7914144369774022	4.101017913656349E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	12115	0.0	22.798595	1
GATCGGT	50	2.7033064E-4	22.2	11
TACGTTA	90	2.1542328E-6	18.5	19
AATACTG	500	0.0	17.39	5
GTATTGG	1450	0.0	17.351723	1
CAGATCG	120	1.04259016E-7	16.958332	9
GTATACT	155	4.0199666E-10	16.709679	4
ACGTTTA	90	4.449249E-5	16.444445	26
ACGTTAA	105	9.3516155E-6	15.857142	20
TGGACCC	2230	0.0	15.596413	5
TTGGACC	2210	0.0	15.570136	4
CTCTAAT	410	0.0	15.341463	1
CCGAGTT	230	0.0	15.282609	13
CCGTTTA	390	0.0	15.179487	27
ATTGGAC	1675	0.0	15.131343	3
GTATCAA	18445	0.0	14.994578	2
TACTGGT	470	0.0	14.957447	7
ACCGTTA	415	0.0	14.710843	8
CTAGGCA	340	0.0	14.691175	4
TGTTACG	240	0.0	14.645833	16
>>END_MODULE