FastQCFastQC Report
Wed 25 May 2016
SRR2088432_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088432_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3136186
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT387871.236757003570579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317061.0109732012068162No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT266730.8504916481356654No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141280.45048348535450383No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG82850.2641743825143024No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA76600.24424571756904725No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG74830.238601919656551No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68080.21707896151567538No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA65600.20917126726539814No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG61240.19526903059958817No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.13717298655118032No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42110.13427137293515115No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA37130.11839221270677186No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC34940.11140920850995445No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33570.10704084515395453No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA33510.10684952997048007No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33200.10586106818919541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108050.021.5904671
TATACCG2050.018.9512215
GTATTGG13950.016.8422951
ATAAGAC1851.8189894E-1116.03
GTATTAG8500.015.8882361
ATTGGAC16250.015.5969233
TAATACT8500.015.2352944
ATTAGAG7350.015.1020413
GATCGGT1353.9782208E-715.07407311
TAACGGC6150.015.04065136
TTAACGG6300.014.9761935
CGATTGC750.004106510414.810
TTGGACC21100.014.46682554
GTATCAA168400.013.8420432
TGGACCC20600.013.7402925
GTAATAC2457.2759576E-1213.5918363
AACGGCC7150.013.19580637
GCACCGC7350.013.08843610
TATTAGA8200.013.0853662
AATACTG9350.013.0588235
Produced by FastQC (version 0.11.5)