##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088432_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3136186
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413171285121482	31.0	31.0	34.0	30.0	34.0
2	31.59818231444181	31.0	31.0	34.0	30.0	34.0
3	31.714778396434394	31.0	31.0	34.0	30.0	34.0
4	35.40700934192041	37.0	35.0	37.0	33.0	37.0
5	35.28965915924629	37.0	35.0	37.0	33.0	37.0
6	35.34334188087059	37.0	35.0	37.0	33.0	37.0
7	35.18077849974459	37.0	35.0	37.0	32.0	37.0
8	35.22139343776166	37.0	35.0	37.0	32.0	37.0
9	36.76033054161966	39.0	37.0	39.0	32.0	39.0
10	36.62048233108623	39.0	35.0	39.0	32.0	39.0
11	36.645292084079195	39.0	35.0	39.0	32.0	39.0
12	36.49853420683595	38.0	35.0	39.0	32.0	39.0
13	36.51528002484547	38.0	35.0	39.0	32.0	39.0
14	37.475255613028054	39.0	36.0	41.0	32.0	41.0
15	37.50066800884896	39.0	36.0	41.0	32.0	41.0
16	37.36625665697124	39.0	36.0	41.0	32.0	41.0
17	37.43517540094879	39.0	36.0	41.0	32.0	41.0
18	37.310959554057064	39.0	36.0	41.0	32.0	41.0
19	37.39412267002021	39.0	36.0	41.0	32.0	41.0
20	37.29296444789946	39.0	36.0	41.0	31.0	41.0
21	37.13858967548481	39.0	36.0	40.0	31.0	41.0
22	37.04028778905333	39.0	36.0	40.0	31.0	41.0
23	36.84410490959401	39.0	36.0	40.0	30.0	41.0
24	37.04528653593888	39.0	36.0	40.0	31.0	41.0
25	37.01151302888285	39.0	36.0	41.0	31.0	41.0
26	36.83037740746244	39.0	36.0	40.0	30.0	41.0
27	36.76858419749339	39.0	35.0	40.0	30.0	41.0
28	36.58086797147873	39.0	35.0	40.0	30.0	41.0
29	36.39961692323096	39.0	35.0	40.0	30.0	41.0
30	36.22720910048065	38.0	35.0	40.0	30.0	41.0
31	35.9341126451046	38.0	35.0	40.0	29.0	41.0
32	35.79205538191931	38.0	34.0	40.0	28.0	41.0
33	35.58129014031693	38.0	34.0	40.0	27.0	41.0
34	35.45605394578	38.0	34.0	40.0	27.0	41.0
35	35.210209470994386	38.0	34.0	40.0	26.0	41.0
36	35.04806475126156	38.0	34.0	40.0	25.0	41.0
37	34.88569970020911	38.0	33.0	40.0	25.0	41.0
38	34.80245463757571	38.0	33.0	40.0	24.0	41.0
39	34.69641883485227	38.0	33.0	40.0	24.0	41.0
40	34.56572091068578	38.0	33.0	40.0	23.0	41.0
41	34.34625784312538	38.0	33.0	40.0	22.0	41.0
42	34.105399042021105	38.0	33.0	40.0	21.0	41.0
43	33.76332717511015	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	6.0
13	7.0
14	10.0
15	20.0
16	51.0
17	116.0
18	283.0
19	703.0
20	1448.0
21	2963.0
22	5644.0
23	9367.0
24	14942.0
25	22640.0
26	32334.0
27	45325.0
28	59394.0
29	77862.0
30	96855.0
31	118566.0
32	144048.0
33	175098.0
34	212907.0
35	256684.0
36	324397.0
37	431235.0
38	549752.0
39	553527.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.47579257097634	21.46240688530591	13.793888500235637	23.267912043482113
2	19.131805320220167	23.616647737092126	34.88501638614546	22.366530556542248
3	19.62396362970819	25.024440514688862	32.50279160738553	22.848804248217423
4	13.816591235341273	16.598154573740207	36.380272088453935	33.204982102464584
5	12.443809136320358	38.86513746314792	34.98810338417428	13.702950016357448
6	32.24709886467193	37.72831075707882	13.612681135621422	16.41190924262783
7	26.119145994529664	32.991825102210136	21.564537307417353	19.32449159584285
8	27.22041996233642	34.41827111019563	18.805963676899264	19.555345250568685
9	24.635528632549217	14.48440876912275	21.717334367285616	39.16272823104242
10	16.629051975871327	27.646542647661843	33.21601461137828	22.508390765088553
11	33.23189377160666	22.6950824982957	23.35457782159604	20.7184459085016
12	21.220584493394206	27.46960798881189	30.679207164370993	20.630600353422913
13	31.37836850237837	21.107007046138207	26.054959750473984	21.459664701009444
14	20.61701697539623	23.034348090323725	27.651580614160004	28.697054320120042
15	22.842490847162765	29.62499673169895	25.42601108480173	22.106501336336557
16	21.95405502097133	28.376314415025128	26.406628943563938	23.263001620439606
17	21.86212807531186	26.809985122055895	27.052094486742817	24.275792315889426
18	22.92440563155374	25.714865125984236	29.348386862258806	22.012342380203215
19	22.916912453534323	24.960828216183607	30.372911555628395	21.74934777465367
20	23.646811764353263	24.741007070371467	30.21762739837497	21.394553766900305
21	22.55972700598753	25.601383336319977	30.63440752557406	21.20448213211844
22	21.51827091888045	26.025784184994126	30.168108651719	22.28783624440642
23	22.386363563895763	26.13872391497188	31.013817420267802	20.461095100864554
24	22.357825715694158	26.326722968599437	28.569574636198237	22.745876679508168
25	22.22425583176508	26.300704103646915	29.830054722519645	21.64498534206836
26	22.02404449225907	27.0091442280528	29.76267989207273	21.2041313876154
27	21.102479253462644	27.01893318827391	29.4067061073546	22.471881450908842
28	21.500925008912102	26.697938196267696	29.634626262600495	22.166510532219707
29	21.91601518532383	26.643604684160955	29.036670656651104	22.403709473864115
30	21.735381766260037	26.75386600157006	30.544872019708013	20.965880212461887
31	22.245141072627707	26.527125623288928	30.41130213577894	20.81643116830443
32	21.742237227001205	26.38309717599658	29.524683803830516	22.349981793171704
33	21.475830834013035	26.42914036348609	30.154907904059264	21.94012089844161
34	20.950383682600457	26.892282536813823	30.52220117046629	21.635132610119427
35	20.618802583775324	28.74966599557552	29.382823595284208	21.24870782536495
36	22.640398241685922	26.55445180866186	29.35951502876424	21.445634920887983
37	21.572859517898493	27.108404922412127	29.329319115639187	21.989416444050196
38	21.42812958160007	25.80363537111638	30.608293003029797	22.159942044253754
39	20.884156743254387	25.798278545979098	31.325246653100297	21.99231805766622
40	20.02805318307014	26.448463197017013	30.932636010746812	22.590847609166037
41	21.227057323768424	25.286765517096242	31.064292742841143	22.421884416294187
42	19.929780950492095	27.14469103554445	30.5204155620872	22.40511245187626
43	20.389096820150336	26.676351466398994	29.930941595938503	23.003610117512164
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	204.0
1	405.0
2	606.0
3	1446.0
4	2286.0
5	2286.0
6	3653.5
7	5021.0
8	5606.5
9	6192.0
10	9477.5
11	12763.0
12	12763.0
13	24313.0
14	35863.0
15	57274.5
16	78686.0
17	71552.0
18	64418.0
19	64418.0
20	68040.5
21	71663.0
22	57438.5
23	43214.0
24	48386.5
25	53559.0
26	53559.0
27	59231.0
28	64903.0
29	71669.0
30	78435.0
31	86793.5
32	95152.0
33	95152.0
34	106300.5
35	117449.0
36	128081.0
37	138713.0
38	148323.5
39	157934.0
40	157934.0
41	163945.0
42	169956.0
43	180689.0
44	191422.0
45	199667.0
46	207912.0
47	207912.0
48	261611.5
49	315311.0
50	299384.5
51	283458.0
52	261545.0
53	239632.0
54	239632.0
55	214341.0
56	189050.0
57	161791.0
58	134532.0
59	119681.0
60	104830.0
61	104830.0
62	93066.0
63	81302.0
64	71106.0
65	60910.0
66	52590.0
67	44270.0
68	44270.0
69	38233.0
70	32196.0
71	26889.0
72	21582.0
73	17882.0
74	14182.0
75	14182.0
76	11296.0
77	8410.0
78	6591.5
79	4773.0
80	3678.5
81	2584.0
82	2584.0
83	1972.0
84	1360.0
85	1037.5
86	715.0
87	523.5
88	332.0
89	332.0
90	291.5
91	251.0
92	170.0
93	89.0
94	68.5
95	48.0
96	48.0
97	29.5
98	11.0
99	9.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3136186.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.024712950290784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.47263442522431	37.37177821249571
2	11.543756537443405	10.856836750826378
3	3.6778041254401144	5.188430498586499
4	1.576011036443743	2.9644586638102917
5	0.8476927109611274	1.9931253201500416
6	0.5437471727207337	1.5341732828834813
7	0.3597805105868253	1.1843002664818159
8	0.25982313212782204	0.9774486564925045
9	0.19387444887562522	0.820520127609489
>10	1.2736057578389	11.644304029640832
>50	0.13917965758014533	4.542879957898251
>100	0.09747546090089695	9.009835566243314
>500	0.009516759720405442	3.1194902990222184
>1k	0.004418495584438003	3.8628258260457633
>5k	4.0786113087120025E-4	1.3719773295202342
>10k+	2.7190742058080016E-4	3.557615212293219
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38787	1.236757003570579	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31706	1.0109732012068162	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	26673	0.8504916481356654	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14128	0.45048348535450383	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8285	0.2641743825143024	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7660	0.24424571756904725	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7483	0.238601919656551	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6808	0.21707896151567538	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6560	0.20917126726539814	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6124	0.19526903059958817	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4302	0.13717298655118032	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4211	0.13427137293515115	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3713	0.11839221270677186	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3494	0.11140920850995445	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3357	0.10704084515395453	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3351	0.10684952997048007	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3320	0.10586106818919541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.1885863912408256E-5	0.0
3	0.0	0.0	0.0	3.1885863912408256E-5	0.0
4	0.0	0.0	0.0	3.1885863912408256E-5	0.0
5	3.1885863912408256E-5	3.1885863912408256E-5	0.0	3.1885863912408256E-5	0.0
6	6.377172782481651E-5	3.1885863912408256E-5	0.0	3.1885863912408256E-5	0.0
7	6.377172782481651E-5	3.1885863912408256E-5	0.0	3.1885863912408256E-5	0.0
8	1.2754345564963302E-4	3.1885863912408256E-5	0.0	3.1885863912408256E-5	0.0
9	1.2754345564963302E-4	3.1885863912408256E-5	0.0	3.188586391240826E-4	0.0
10	1.2754345564963302E-4	3.1885863912408256E-5	0.0	4.464020947737156E-4	0.0
11	1.2754345564963302E-4	3.1885863912408256E-5	0.0	5.739455504233486E-4	0.0
12	1.2754345564963302E-4	3.1885863912408256E-5	0.0	7.333748699853899E-4	0.0
13	1.9131518347444953E-4	3.1885863912408256E-5	0.0	7.652607338977981E-4	0.0
14	1.9131518347444953E-4	3.1885863912408256E-5	0.0	0.0010522335091094725	0.0
15	1.9131518347444953E-4	3.1885863912408256E-5	0.0	0.0014667497399707798	0.0
16	1.9131518347444953E-4	3.1885863912408256E-5	0.0	0.0017856083790948624	0.0
17	2.232010473868578E-4	3.1885863912408256E-5	0.0	0.0025189832490802523	0.0
18	2.232010473868578E-4	3.1885863912408256E-5	0.0	0.002965385343853968	0.0
19	2.232010473868578E-4	6.377172782481651E-5	0.0	0.003921961261226216	0.0
20	2.232010473868578E-4	6.377172782481651E-5	0.0	0.006185857599007202	0.0
21	2.232010473868578E-4	6.377172782481651E-5	0.0	0.01007593299632101	0.0
22	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.0173777958322625	0.0
23	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.022256333010860965	0.0
24	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.029558195846802453	0.0
25	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.035170107895386306	0.0
26	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.04473586706910879	0.0
27	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.09297917916858248	0.0
28	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.19750104107345673	0.0
29	2.5508691129926605E-4	6.377172782481651E-5	0.0	0.3294447459430021	0.0
30	2.869727752116743E-4	6.377172782481651E-5	0.0	0.5090897032255102	0.0
31	3.188586391240826E-4	6.377172782481651E-5	0.0	0.7386998092587621	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10805	0.0	21.590467	1
TATACCG	205	0.0	18.951221	5
GTATTGG	1395	0.0	16.842295	1
ATAAGAC	185	1.8189894E-11	16.0	3
GTATTAG	850	0.0	15.888236	1
ATTGGAC	1625	0.0	15.596923	3
TAATACT	850	0.0	15.235294	4
ATTAGAG	735	0.0	15.102041	3
GATCGGT	135	3.9782208E-7	15.074073	11
TAACGGC	615	0.0	15.040651	36
TTAACGG	630	0.0	14.97619	35
CGATTGC	75	0.0041065104	14.8	10
TTGGACC	2110	0.0	14.4668255	4
GTATCAA	16840	0.0	13.842043	2
TGGACCC	2060	0.0	13.740292	5
GTAATAC	245	7.2759576E-12	13.591836	3
AACGGCC	715	0.0	13.195806	37
GCACCGC	735	0.0	13.088436	10
TATTAGA	820	0.0	13.085366	2
AATACTG	935	0.0	13.058823	5
>>END_MODULE