FastQCFastQC Report
Wed 25 May 2016
SRR2088431_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088431_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3233950
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT325421.006261692357643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280180.8663708467972603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227270.7027628751217551No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119410.3692388565067487No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG83060.25683761344485845No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA75190.2325020485783639No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68800.2127429304720234No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG67720.2094033612146137No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61530.19026268185964534No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55500.17161675350577468No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA42310.13083071785278066No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35320.10921628349232362No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32700.10111473584934831No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32620.10086736034879945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96900.022.7002051
GTATTGG14050.018.697511
ATTGGAC18550.015.25875953
GTATTAG8550.015.1461991
GTATCAA146700.015.0446482
TTGGACC21850.014.9016034
TGGACCC21000.014.8880955
TTAACGG5450.014.5963335
CTAATAC9450.014.0952393
ATTAGAG7450.013.906043
GGACCCT23450.013.8848626
CTAGTAC5300.013.6132083
TGTTACG2601.8189894E-1213.51923116
TATTGGA19850.013.3274552
CTCTAAT7500.013.321
ACCGTTA4450.013.3033718
CCGTATA1409.570831E-613.2142852
CCGTTTA4350.013.18390827
TAACGGC6200.013.12903236
CGCCGTT4700.012.98936225
Produced by FastQC (version 0.11.5)