##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088431_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3233950
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.391495539510505	31.0	31.0	34.0	30.0	34.0
2	31.582368620417757	31.0	31.0	34.0	30.0	34.0
3	31.706044311136537	31.0	31.0	34.0	30.0	34.0
4	35.40311940506192	37.0	35.0	37.0	33.0	37.0
5	35.27567649468916	37.0	35.0	37.0	33.0	37.0
6	35.32535506114813	37.0	35.0	37.0	33.0	37.0
7	35.16844447193061	37.0	35.0	37.0	32.0	37.0
8	35.200080706257054	37.0	35.0	37.0	32.0	37.0
9	36.75353236753815	39.0	35.0	39.0	32.0	39.0
10	36.598144683745886	39.0	35.0	39.0	32.0	39.0
11	36.62515221323768	39.0	35.0	39.0	32.0	39.0
12	36.47916665378253	38.0	35.0	39.0	32.0	39.0
13	36.49594644320413	38.0	35.0	39.0	32.0	39.0
14	37.47934971165293	39.0	36.0	41.0	32.0	41.0
15	37.497388333152955	39.0	36.0	41.0	32.0	41.0
16	37.36170781861191	39.0	36.0	41.0	32.0	41.0
17	37.41598076655483	39.0	36.0	41.0	32.0	41.0
18	37.281515174940864	39.0	36.0	40.0	31.0	41.0
19	37.35028772862908	39.0	36.0	41.0	31.0	41.0
20	37.24844756412437	39.0	36.0	40.0	31.0	41.0
21	37.09326705731381	39.0	36.0	40.0	31.0	41.0
22	37.001917469348626	39.0	36.0	40.0	31.0	41.0
23	36.818602328421896	39.0	36.0	40.0	30.0	41.0
24	37.03591459360844	39.0	36.0	40.0	31.0	41.0
25	37.00431330107145	39.0	36.0	40.0	31.0	41.0
26	36.8357179300855	39.0	36.0	40.0	30.0	41.0
27	36.783675072280026	39.0	35.0	40.0	30.0	41.0
28	36.60057638491628	39.0	35.0	40.0	30.0	41.0
29	36.444824440699456	39.0	35.0	40.0	30.0	41.0
30	36.30241191113035	38.0	35.0	40.0	30.0	41.0
31	36.04667975695357	38.0	35.0	40.0	29.0	41.0
32	35.91671176115896	38.0	35.0	40.0	29.0	41.0
33	35.72082994480434	38.0	34.0	40.0	27.0	41.0
34	35.62085375469627	38.0	34.0	40.0	27.0	41.0
35	35.376748249045285	38.0	34.0	40.0	26.0	41.0
36	35.22648092889501	38.0	34.0	40.0	25.0	41.0
37	35.08828584239088	38.0	34.0	40.0	25.0	41.0
38	35.01940444348243	38.0	33.0	40.0	25.0	41.0
39	34.94291222808021	38.0	33.0	40.0	24.0	41.0
40	34.83766415683607	38.0	33.0	40.0	24.0	41.0
41	34.64868782757927	38.0	33.0	40.0	23.0	41.0
42	34.44125048315527	38.0	33.0	40.0	23.0	41.0
43	34.11494580930441	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	4.0
14	5.0
15	23.0
16	65.0
17	133.0
18	295.0
19	699.0
20	1445.0
21	2953.0
22	5361.0
23	9289.0
24	14772.0
25	21961.0
26	32299.0
27	44311.0
28	59847.0
29	78133.0
30	98005.0
31	120413.0
32	146668.0
33	178472.0
34	217025.0
35	264763.0
36	333200.0
37	441486.0
38	581915.0
39	580404.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.37859274262125	21.351257749810603	13.371789916356159	23.898359591211985
2	19.197266500718936	23.31133752840953	34.688538783840194	22.80285718703134
3	19.561094018151177	24.99117178682416	32.15467153171818	23.293062663306483
4	13.959399495972418	16.475981384993585	36.49506022047341	33.06955889856059
5	12.618315063621887	38.57016960682756	35.14247282734736	13.669042502203189
6	32.5819508650412	37.568144219916825	13.785247143586016	16.06465777145596
7	26.57440591227446	32.5001932621098	21.658745496992843	19.266655328622893
8	27.080165123146614	34.486031014703386	18.953570710740735	19.48023315140927
9	24.880749547766662	14.327772538227245	21.309822353468668	39.48165556053742
10	16.42675366038436	27.37008920978988	33.712642434174924	22.49051469565083
11	33.702654648340264	22.415374387359112	23.269036317815676	20.61293464648495
12	21.156665996691356	26.929080536186394	30.763091575318107	21.15116189180414
13	31.205337126424343	20.91151687564743	25.910542834614013	21.972603163314215
14	20.89880795930673	22.477403794121738	27.29395939949597	29.329828847075557
15	23.076485412575952	29.387436416765873	24.911640563397704	22.624437607260468
16	22.486865907017734	28.1248009400269	25.99019774579075	23.398135407164613
17	22.134726881986424	26.710524281451477	26.694568561666077	24.460180274896025
18	23.14315310997387	25.750429041883766	28.778397934414567	22.328019913727797
19	23.191484098393605	24.869153821178436	29.650118276411202	22.28924380401676
20	23.90772893829527	24.716059308276257	29.525812087385393	21.850399666043074
21	22.93170890087973	25.474357983271233	29.861500641630208	21.732432474218836
22	21.832990615191946	25.90859475254719	29.501785741894587	22.756628890366272
23	22.724531919170055	25.973345289815857	30.317290001391488	20.984832789622597
24	22.760772430000465	26.11063869262048	28.060978060885294	23.06761081649376
25	22.720604833098843	26.08082994480434	29.05851976684859	22.140045455248224
26	22.566149754943645	26.684488010018708	29.072867545880428	21.676494689157224
27	21.606085437313503	26.828213175837597	28.660585352278172	22.905116034570728
28	22.020779542046103	26.443204131170862	28.910434607832524	22.62558171895051
29	22.402263485830023	26.453068229255244	28.436463148780906	22.70820513613383
30	22.235625164272793	26.49651355153914	29.778660770883903	21.489200513304162
31	22.763586326319206	26.214721934476415	29.65855996536743	21.363131773836948
32	22.19994124831862	26.064936068894077	28.856259373212325	22.878863309574978
33	22.02662378824657	26.203930178264972	29.43264428949118	22.336801743997277
34	21.62522611666847	26.638476166916618	29.74628550224957	21.99001221416534
35	21.237310409870283	28.434422300901375	28.717883702592808	21.61038358663554
36	23.135082484268466	26.432195921396435	28.62533434344996	21.807387250885142
37	22.09310595401908	26.890211660662654	28.683962337080043	22.332720048238222
38	22.024490174554337	25.78824657153017	29.813231497085606	22.374031756829883
39	21.53218200652453	25.705623154346853	30.429938619953926	22.332256219174692
40	20.71661590315249	26.30897200018553	30.115122373567928	22.859289723094047
41	21.802841726062557	25.19111303514278	30.261754201518265	22.744291037276394
42	20.64800012368775	26.95592077799595	29.77142503749285	22.624654060823453
43	21.047295103511185	26.46491133134402	29.21820065245288	23.26959291269191
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	202.0
1	366.0
2	530.0
3	1233.5
4	1937.0
5	1937.0
6	3156.5
7	4376.0
8	4809.5
9	5243.0
10	7920.0
11	10597.0
12	10597.0
13	20255.5
14	29914.0
15	47936.0
16	65958.0
17	60557.5
18	55157.0
19	55157.0
20	59573.5
21	63990.0
22	52427.5
23	40865.0
24	46632.5
25	52400.0
26	52400.0
27	58717.5
28	65035.0
29	72127.0
30	79219.0
31	89651.5
32	100084.0
33	100084.0
34	112009.5
35	123935.0
36	136485.5
37	149036.0
38	158806.5
39	168577.0
40	168577.0
41	173925.0
42	179273.0
43	190052.5
44	200832.0
45	209034.5
46	217237.0
47	217237.0
48	266371.0
49	315505.0
50	301268.0
51	287031.0
52	266863.5
53	246696.0
54	246696.0
55	222764.0
56	198832.0
57	172930.0
58	147028.0
59	132263.0
60	117498.0
61	117498.0
62	104917.5
63	92337.0
64	80609.5
65	68882.0
66	58973.5
67	49065.0
68	49065.0
69	42236.5
70	35408.0
71	29826.5
72	24245.0
73	20049.5
74	15854.0
75	15854.0
76	12720.0
77	9586.0
78	7525.5
79	5465.0
80	4192.5
81	2920.0
82	2920.0
83	2246.0
84	1572.0
85	1164.5
86	757.0
87	580.0
88	403.0
89	403.0
90	347.0
91	291.0
92	198.0
93	105.0
94	81.5
95	58.0
96	58.0
97	33.5
98	9.0
99	7.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3233950.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.995559241020096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00200135723004	39.99744798255564
2	11.449299025631236	11.448282154082003
3	3.616197049549324	5.4238138145383585
4	1.543407716787641	3.086541277508163
5	0.8179413698900304	2.044671810700907
6	0.4795055106460778	1.4383887698340942
7	0.33055134582860907	1.1568269574801202
8	0.2256908070721446	0.9026830492103233
9	0.1851073021021255	0.8329088779372997
>10	1.134345552573473	10.903606542568788
>50	0.12297181501826317	4.241018064422362
>100	0.07995442621442701	7.8401496682439324
>500	0.008498384950422455	2.937823030088408
>1k	0.003908016436848978	3.5153827619105593
>5k	3.721920416046646E-4	1.2771255845601435
>10k+	2.481280277364431E-4	2.9533296543587504
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	32542	1.006261692357643	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28018	0.8663708467972603	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	22727	0.7027628751217551	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11941	0.3692388565067487	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	8306	0.25683761344485845	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7519	0.2325020485783639	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6880	0.2127429304720234	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6772	0.2094033612146137	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6153	0.19026268185964534	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5550	0.17161675350577468	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4231	0.13083071785278066	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3532	0.10921628349232362	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3270	0.10111473584934831	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3262	0.10086736034879945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	3.092193756860805E-5	0.0
9	0.0	0.0	0.0	1.236877502744322E-4	0.0
10	3.092193756860805E-5	0.0	0.0	1.5460968784304025E-4	0.0
11	3.092193756860805E-5	0.0	0.0	3.092193756860805E-4	0.0
12	6.18438751372161E-5	0.0	0.0	5.565948762349448E-4	0.0
13	6.18438751372161E-5	0.0	0.0	6.18438751372161E-4	0.0
14	6.18438751372161E-5	0.0	0.0	7.112045640779852E-4	0.0
15	6.18438751372161E-5	0.0	0.0	8.967361894896334E-4	0.0
16	6.18438751372161E-5	0.0	0.0	0.00126779944031293	0.0
17	9.276581270582414E-5	0.0	0.0	0.0017934723789792668	0.0
18	9.276581270582414E-5	0.0	0.0	0.0020717698170967394	0.0
19	1.236877502744322E-4	0.0	0.0	0.00253559888062586	0.0
20	1.236877502744322E-4	0.0	0.0	0.004390915134742343	0.0
21	1.236877502744322E-4	0.0	0.0	0.007359421141328716	0.0
22	1.236877502744322E-4	0.0	0.0	0.011688492400933842	0.0
23	1.5460968784304025E-4	3.092193756860805E-5	0.0	0.015399124909166808	0.0
24	1.855316254116483E-4	3.092193756860805E-5	0.0	0.020686776233398783	0.0
25	1.855316254116483E-4	3.092193756860805E-5	0.0	0.024428330679200357	0.0
26	1.855316254116483E-4	3.092193756860805E-5	0.0	0.03218973700892098	0.0
27	1.855316254116483E-4	3.092193756860805E-5	0.0	0.07446002566520818	0.0
28	1.855316254116483E-4	3.092193756860805E-5	0.0	0.16156712379597707	0.0
29	1.855316254116483E-4	3.092193756860805E-5	0.0	0.26840241809551785	0.0
30	2.1645356298025635E-4	3.092193756860805E-5	0.0	0.4088807804697042	0.0
31	2.1645356298025635E-4	3.092193756860805E-5	0.0	0.5793534222854404	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9690	0.0	22.700205	1
GTATTGG	1405	0.0	18.69751	1
ATTGGAC	1855	0.0	15.2587595	3
GTATTAG	855	0.0	15.146199	1
GTATCAA	14670	0.0	15.044648	2
TTGGACC	2185	0.0	14.901603	4
TGGACCC	2100	0.0	14.888095	5
TTAACGG	545	0.0	14.59633	35
CTAATAC	945	0.0	14.095239	3
ATTAGAG	745	0.0	13.90604	3
GGACCCT	2345	0.0	13.884862	6
CTAGTAC	530	0.0	13.613208	3
TGTTACG	260	1.8189894E-12	13.519231	16
TATTGGA	1985	0.0	13.327455	2
CTCTAAT	750	0.0	13.32	1
ACCGTTA	445	0.0	13.303371	8
CCGTATA	140	9.570831E-6	13.214285	2
CCGTTTA	435	0.0	13.183908	27
TAACGGC	620	0.0	13.129032	36
CGCCGTT	470	0.0	12.989362	25
>>END_MODULE