FastQCFastQC Report
Wed 25 May 2016
SRR2088430_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088430_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043633
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT380651.2506435565654597No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT333461.0955985823520773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT272120.8940631147053537No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140800.46260505126603635No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG111540.3664699390498132No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA94440.3102870812611113No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG86750.285021222992391No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA82020.2694805845514226No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA75960.24957016828244405No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG69870.22956118559629232No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA54110.17778096110799166No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42310.13901150368654827No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT42210.13868294896263775No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41770.13723730817743138No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC41590.1366459096743924No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38750.12731495551533317No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36560.12011960706169239No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35590.11693262623976017No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA35300.11597981754041962No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33690.11069008648545997No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33050.10858733625243254No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG32030.10523607806854506No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC30900.10152340968835598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108850.022.6724851
TAGGTCG1306.9849193E-1018.521
ATTAGAG5900.016.6186453
GTATTAG7350.016.1088431
GCCTAAT1659.767973E-1015.696971
GTATTGG14200.015.37323951
TACTCCG6550.015.2519085
AGCGTAT855.367759E-415.2352947
CGAATTA1353.9781116E-715.07407315
TTAACGG5000.014.80000135
ACGTTTA1651.6481863E-814.57575726
CCGGTCT6250.014.5049
TAATACT9350.014.443854
CTAATAC8600.014.412793
GTATCAA171400.014.387692
GTCGGGT2450.014.34693824
ACCGTTA3750.014.3066668
TCCGGTC6650.014.187978
TAACGGC5100.014.147058536
TACTGGT8250.014.1272747
Produced by FastQC (version 0.11.5)