##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088430_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3043633
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413786419059065	31.0	31.0	34.0	30.0	34.0
2	31.597541819266645	31.0	31.0	34.0	30.0	34.0
3	31.712708463865386	31.0	31.0	34.0	30.0	34.0
4	35.395989923883725	37.0	35.0	37.0	33.0	37.0
5	35.291226964617614	37.0	35.0	37.0	33.0	37.0
6	35.35251293437809	37.0	35.0	37.0	33.0	37.0
7	35.18253021964212	37.0	35.0	37.0	32.0	37.0
8	35.234715223550275	37.0	35.0	37.0	32.0	37.0
9	36.76714045353037	39.0	37.0	39.0	32.0	39.0
10	36.63315058024407	39.0	35.0	39.0	32.0	39.0
11	36.66291041002644	39.0	35.0	39.0	32.0	39.0
12	36.517796988007426	38.0	35.0	39.0	32.0	39.0
13	36.53294040378719	38.0	35.0	39.0	32.0	39.0
14	37.48930537945935	39.0	36.0	41.0	32.0	41.0
15	37.51661090545411	39.0	36.0	41.0	32.0	41.0
16	37.38246628289284	39.0	36.0	41.0	32.0	41.0
17	37.46372936553126	39.0	36.0	41.0	32.0	41.0
18	37.340736218854246	39.0	36.0	41.0	32.0	41.0
19	37.424525887319525	39.0	36.0	41.0	32.0	41.0
20	37.33297214217352	39.0	36.0	41.0	31.0	41.0
21	37.18065121517608	39.0	36.0	40.0	31.0	41.0
22	37.096590489063566	39.0	36.0	40.0	31.0	41.0
23	36.89251365062739	39.0	36.0	40.0	30.0	41.0
24	37.09027862426252	39.0	36.0	41.0	31.0	41.0
25	37.064660555329766	39.0	36.0	41.0	31.0	41.0
26	36.88671597396927	39.0	36.0	40.0	30.0	41.0
27	36.834397248288475	39.0	35.0	40.0	30.0	41.0
28	36.64554070743746	39.0	35.0	40.0	30.0	41.0
29	36.47906728570757	39.0	35.0	40.0	30.0	41.0
30	36.30446936276483	38.0	35.0	40.0	30.0	41.0
31	36.01293487092563	38.0	35.0	40.0	29.0	41.0
32	35.872527995326635	38.0	35.0	40.0	29.0	41.0
33	35.662420206378364	38.0	34.0	40.0	27.0	41.0
34	35.54359181938164	38.0	34.0	40.0	27.0	41.0
35	35.28796770175642	38.0	34.0	40.0	26.0	41.0
36	35.13633838245281	38.0	34.0	40.0	25.0	41.0
37	34.98387650547881	38.0	33.0	40.0	25.0	41.0
38	34.897050662809875	38.0	33.0	40.0	24.0	41.0
39	34.7962566446086	38.0	33.0	40.0	24.0	41.0
40	34.659389617605015	38.0	33.0	40.0	24.0	41.0
41	34.440556072299124	38.0	33.0	40.0	23.0	41.0
42	34.19344743600822	38.0	33.0	40.0	21.0	41.0
43	33.85679745225525	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	0.0
13	1.0
14	8.0
15	22.0
16	39.0
17	107.0
18	254.0
19	589.0
20	1322.0
21	2845.0
22	4987.0
23	8792.0
24	14033.0
25	21211.0
26	30043.0
27	42305.0
28	57093.0
29	73892.0
30	92749.0
31	112689.0
32	137866.0
33	168567.0
34	205277.0
35	248164.0
36	315300.0
37	423356.0
38	538557.0
39	543563.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.40581338157393	21.605692933412143	13.90213603282656	23.08635765218737
2	18.80476391207481	23.828267074249755	35.25556464922019	22.111404364455243
3	19.62624928826833	25.1733372584671	32.81906852764443	22.38134492562014
4	13.668829323377688	16.457766097292282	36.612955635584186	33.26044894374584
5	12.268759078377716	38.88691573524141	35.14336321100474	13.700961975376138
6	32.099829381531876	37.50330608190935	13.495122440846185	16.901742095712592
7	25.99360698218215	33.07501265757074	21.541526195832414	19.389854164414697
8	27.134776104740617	34.44709004009353	18.91762245973808	19.500511395427765
9	24.38835431210005	14.553134362782899	21.910000318698085	39.14851100641897
10	16.378288709578325	27.60819060642331	33.5896936325766	22.42382705142177
11	32.82268262960745	22.53487854810353	23.83260399660537	20.809834825683648
12	21.137798151091147	27.287422629469454	31.0673790171154	20.507400202324
13	31.589156774157722	20.969906687172863	26.510160719114296	20.930775819555116
14	20.457722728068724	23.05425785566131	27.879511097428633	28.60850831884133
15	22.47396450229052	29.626830830129652	25.806166512191187	22.09303815538864
16	21.70761060876919	28.539643248709684	26.720928574502906	23.03181756801822
17	21.672225265004027	26.80369150945597	26.988897807324342	24.535185418215665
18	23.04147707690119	25.59513581302345	29.60820177728392	21.755185332791438
19	22.634693473227554	24.671009941080282	30.82881543208396	21.865481153608204
20	23.636621103792738	24.407114786835336	30.7588989868358	21.197365122536127
21	22.40135390830629	25.376449788788598	31.3237174127104	20.89847889019471
22	21.134972580465515	25.74436536862362	30.791885881116414	22.328776169794455
23	22.244863293307702	25.96663263934909	31.723239956985616	20.06526411035759
24	22.16032616284552	25.96341280305477	29.08760681724768	22.78865421685203
25	22.05292162359917	26.229279285643177	30.22729744354855	21.490501647209108
26	21.842383756517293	26.869599587072422	30.31985130927415	20.968165347136136
27	20.748559369674332	27.006836895249855	29.888196113000483	22.356407622075327
28	21.314757725389363	26.473953988539357	30.047709431459047	22.163578854612233
29	21.678730648537456	26.401244828137955	29.443957270801047	22.476067252523546
30	21.547144481611287	26.59328506426366	31.14895915506239	20.710611299062666
31	22.27758734380919	26.383929994187866	30.836897878292156	20.501584783710783
32	21.90802899035462	26.119640574274232	29.809014424538045	22.163316010833107
33	21.490370225319545	26.312929318350797	30.427978668913102	21.76872178741655
34	20.83891191874973	27.056678646867084	30.850631465751622	21.253777968631564
35	20.394673076550294	29.2076278578922	29.538285332035763	20.859413733521748
36	22.643695872662704	26.64470387855566	29.623381005528593	21.088219243253047
37	21.571227542873928	27.303423244523895	29.54679489938504	21.578554313217133
38	21.43573157473322	25.824664143147352	30.860258119162197	21.879346162957226
39	20.956567365382096	25.739995590795605	31.59089811419445	21.71253892962785
40	19.85567905197506	26.542194804695573	31.077399936194674	22.524726207134695
41	21.543596090593052	25.254227431493874	31.060577934330453	22.141598543582617
42	19.98831659401774	27.388518918016725	30.55378227269845	22.069382215267083
43	20.50487033094989	27.162177568714757	29.693593150028274	22.639358950307084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	381.0
2	566.0
3	1371.5
4	2177.0
5	2177.0
6	3503.5
7	4830.0
8	5452.0
9	6074.0
10	9266.5
11	12459.0
12	12459.0
13	23630.5
14	34802.0
15	55742.0
16	76682.0
17	70712.5
18	64743.0
19	64743.0
20	69429.0
21	74115.0
22	59116.0
23	44117.0
24	49496.0
25	54875.0
26	54875.0
27	60357.5
28	65840.0
29	72066.0
30	78292.0
31	86041.0
32	93790.0
33	93790.0
34	104142.0
35	114494.0
36	124864.5
37	135235.0
38	142427.5
39	149620.0
40	149620.0
41	154620.0
42	159620.0
43	169647.0
44	179674.0
45	189986.0
46	200298.0
47	200298.0
48	261586.5
49	322875.0
50	303353.0
51	283831.0
52	260554.5
53	237278.0
54	237278.0
55	209370.0
56	181462.0
57	152347.0
58	123232.0
59	109097.0
60	94962.0
61	94962.0
62	84576.5
63	74191.0
64	64905.0
65	55619.0
66	47691.0
67	39763.0
68	39763.0
69	34193.5
70	28624.0
71	24187.0
72	19750.0
73	16222.0
74	12694.0
75	12694.0
76	10204.0
77	7714.0
78	5984.0
79	4254.0
80	3313.0
81	2372.0
82	2372.0
83	1805.5
84	1239.0
85	952.5
86	666.0
87	517.5
88	369.0
89	369.0
90	262.0
91	155.0
92	105.0
93	55.0
94	39.0
95	23.0
96	23.0
97	13.5
98	4.0
99	3.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3043633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.031937229692566
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76247458387653	36.2558928610026
2	11.849482843985868	10.909093009573528
3	3.778000598412036	5.217260591995313
4	1.7013284395524928	3.132617757462846
5	0.8892695793910403	2.046740072940174
6	0.5492826468684254	1.5170726593204045
7	0.36721704868648253	1.1832598494366384
8	0.27112395994571775	0.9984288884549568
9	0.21794836836720294	0.90293270507937
>10	1.361450755085849	12.091126062364323
>50	0.14524963101638108	4.612593996627122
>100	0.09308306769918061	8.428046679277742
>500	0.008625598232308688	2.7409230096338772
>1k	0.0046721990424858076	4.333403314926381
>5k	4.3127991161407455E-4	1.532459127343646
>10k+	3.593999263450621E-4	4.0981494145612
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	38065	1.2506435565654597	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33346	1.0955985823520773	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	27212	0.8940631147053537	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14080	0.46260505126603635	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11154	0.3664699390498132	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	9444	0.3102870812611113	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8675	0.285021222992391	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	8202	0.2694805845514226	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7596	0.24957016828244405	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6987	0.22956118559629232	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5411	0.17778096110799166	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4231	0.13901150368654827	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4221	0.13868294896263775	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4177	0.13723730817743138	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4159	0.1366459096743924	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3875	0.12731495551533317	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3656	0.12011960706169239	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3559	0.11693262623976017	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3530	0.11597981754041962	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3369	0.11069008648545997	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3305	0.10858733625243254	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3203	0.10523607806854506	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	3090	0.10152340968835598	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.285547239105372E-5	0.0	0.0	0.0	0.0
4	3.285547239105372E-5	0.0	0.0	0.0	0.0
5	6.571094478210744E-5	0.0	0.0	0.0	0.0
6	1.3142188956421488E-4	0.0	0.0	0.0	0.0
7	1.3142188956421488E-4	0.0	0.0	0.0	0.0
8	1.642773619552686E-4	0.0	0.0	0.0	0.0
9	1.642773619552686E-4	0.0	0.0	1.642773619552686E-4	0.0
10	2.29988306737376E-4	0.0	0.0	2.29988306737376E-4	0.0
11	2.6284377912842976E-4	0.0	0.0	4.928320858658058E-4	0.0
12	2.6284377912842976E-4	0.0	0.0	6.242539754300207E-4	0.0
13	2.956992515194835E-4	0.0	0.0	6.242539754300207E-4	0.0
14	3.285547239105372E-4	0.0	3.285547239105372E-5	9.19953226949504E-4	0.0
15	3.285547239105372E-4	0.0	3.285547239105372E-5	0.001182797006077934	0.0
16	3.285547239105372E-4	0.0	3.285547239105372E-5	0.0018070509815079545	0.0
17	3.285547239105372E-4	0.0	3.285547239105372E-5	0.002661293263675351	0.0
18	3.614101963015909E-4	0.0	3.285547239105372E-5	0.0034169691286695864	0.0
19	3.614101963015909E-4	0.0	3.285547239105372E-5	0.004566910662356467	0.0
20	3.9426566869264464E-4	0.0	3.285547239105372E-5	0.007721036011897624	0.0
21	4.271211410836983E-4	0.0	3.285547239105372E-5	0.013175044428812541	0.0
22	4.59976613474752E-4	0.0	3.285547239105372E-5	0.021717467250486507	0.0
23	4.928320858658058E-4	0.0	3.285547239105372E-5	0.02733575302935669	0.0
24	4.928320858658058E-4	0.0	3.285547239105372E-5	0.03558247659951118	0.0
25	4.928320858658058E-4	0.0	6.571094478210744E-5	0.04198929371576665	0.0
26	4.928320858658058E-4	0.0	6.571094478210744E-5	0.051385958819608016	0.0
27	4.928320858658058E-4	0.0	6.571094478210744E-5	0.0985335617007701	0.0
28	4.928320858658058E-4	0.0	6.571094478210744E-5	0.19893988532783027	0.0
29	4.928320858658058E-4	0.0	6.571094478210744E-5	0.32674767292902923	0.0
30	4.928320858658058E-4	0.0	6.571094478210744E-5	0.4971361527490338	0.0
31	4.928320858658058E-4	0.0	6.571094478210744E-5	0.6900306311569102	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10885	0.0	22.672485	1
TAGGTCG	130	6.9849193E-10	18.5	21
ATTAGAG	590	0.0	16.618645	3
GTATTAG	735	0.0	16.108843	1
GCCTAAT	165	9.767973E-10	15.69697	1
GTATTGG	1420	0.0	15.3732395	1
TACTCCG	655	0.0	15.251908	5
AGCGTAT	85	5.367759E-4	15.235294	7
CGAATTA	135	3.9781116E-7	15.074073	15
TTAACGG	500	0.0	14.800001	35
ACGTTTA	165	1.6481863E-8	14.575757	26
CCGGTCT	625	0.0	14.504	9
TAATACT	935	0.0	14.44385	4
CTAATAC	860	0.0	14.41279	3
GTATCAA	17140	0.0	14.38769	2
GTCGGGT	245	0.0	14.346938	24
ACCGTTA	375	0.0	14.306666	8
TCCGGTC	665	0.0	14.18797	8
TAACGGC	510	0.0	14.1470585	36
TACTGGT	825	0.0	14.127274	7
>>END_MODULE