FastQCFastQC Report
Wed 25 May 2016
SRR2088429_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088429_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3350841
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT453821.3543465655338465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT386771.1542475456161603No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT319260.9527757360017977No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165390.49357758246362626No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG118090.3524189897401876No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109160.325768963672105No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG100370.29953674316388035No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93950.28037737391896544No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA90330.26957411587120966No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG82490.24617700451916397No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA61410.18326742450626574No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.1456947673733251No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47120.14062141414647847No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA45510.13581665020811193No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC45270.13510041210549828No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG40890.12202906673279931No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39910.11910442781379361No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39440.1177017948628419No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC39400.11758242184573962No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39060.1165677512003703No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA38890.11606041587768562No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.11050957058242991No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC34440.10278016772505769No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG33940.10128800501127927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121650.023.6325511
GTATTGG15950.018.0940461
TCTAGAC2450.017.3673483
GTATTAG8750.016.0685711
TAATACT10000.015.7250014
TGGACCC23350.015.6081385
GTATCAA189350.015.2123062
TTGGACC24450.015.2085884
ATTGGAC18350.015.1226163
GTCAACC7400.015.015
TATTACC8050.014.9378882
CTAATAC10850.014.8341023
TATACTG3500.014.85
ATTAGAG7800.014.7051293
ATTACCC8250.014.5757563
TCTAATA10600.014.3113212
TACCCTC9200.014.2771735
GGACCCT25550.014.1193736
TCGGGTA2950.013.7966125
ATACTGG10500.013.5666676
Produced by FastQC (version 0.11.5)