FastQCFastQC Report
Wed 25 May 2016
SRR2088428_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088428_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1340725
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT187341.3973036976262843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT160531.1973372615562476No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT124400.9278561972067352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66950.4993566913423707No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG51340.38292714762535196No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44400.3311641089708926No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42430.31647056629808495No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG40340.3008819854929236No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38140.28447295306643794No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG31860.23763262413992428No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA23720.1769192041619273No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.16013723918029424No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18410.13731376680527327No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18410.13731376680527327No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC17890.1334352682317403No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA17070.1273191743273229No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17020.1269462417721755No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG16990.12672248223908705No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC16670.1243357138861437No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16170.12060638833466969No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16010.11941300415819799No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15410.11493781349642916No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA14180.1057636726398031No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC13970.10419735590818402No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13620.10158682802215219No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52300.022.9216061
TAAAGCC1403.6379788E-1219.8214284
AACGGAC609.237909E-418.526
ACGGACC609.237909E-418.527
GACGCAA500.00703547718.510
ACCGTTA1502.5102054E-1017.2666668
CGGACCA650.001580119617.07692328
GTATTGG6700.016.0149251
TAGTACT2200.015.9772734
CCGTTTA1403.474088E-815.85714227
TTTGCGC957.061077E-515.5789487
GTATCAA78350.015.3005752
TAGGCGA855.364978E-415.23529433
TCGAACC2500.014.80000131
TGTTACG1001.0935791E-414.816
GTGCGCC1508.103234E-814.811
CTGTGCG1001.0935791E-414.89
TTATGCG1405.9964805E-714.5357144
TCGTTAT1152.2102768E-514.4782613
ATTGGAC8000.014.1062513
Produced by FastQC (version 0.11.5)