##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088428_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1340725
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.04164388670309	31.0	31.0	33.0	30.0	34.0
2	31.260673143262043	31.0	31.0	34.0	28.0	34.0
3	31.35508847824871	31.0	31.0	34.0	28.0	34.0
4	35.07699490947062	35.0	35.0	37.0	32.0	37.0
5	34.93347778254303	35.0	35.0	37.0	32.0	37.0
6	35.0206992485409	36.0	35.0	37.0	32.0	37.0
7	34.837986910067315	35.0	35.0	37.0	32.0	37.0
8	34.90683398907308	36.0	35.0	37.0	32.0	37.0
9	36.36820749967369	38.0	35.0	39.0	32.0	39.0
10	36.19874918421004	38.0	35.0	39.0	32.0	39.0
11	36.259190363422775	38.0	35.0	39.0	32.0	39.0
12	36.08670308974622	38.0	35.0	39.0	31.0	39.0
13	36.13024371142479	38.0	35.0	39.0	31.0	39.0
14	36.94859348486826	39.0	36.0	40.0	31.0	41.0
15	36.98274068134778	38.0	36.0	40.0	31.0	41.0
16	36.826598295698226	38.0	36.0	40.0	31.0	41.0
17	36.94275783624531	38.0	36.0	40.0	31.0	41.0
18	36.80797031456861	38.0	36.0	40.0	31.0	41.0
19	36.917460329299445	39.0	36.0	40.0	31.0	41.0
20	36.82830259747524	39.0	36.0	40.0	30.0	41.0
21	36.66642823845308	39.0	35.0	40.0	30.0	41.0
22	36.58507896846855	38.0	35.0	40.0	30.0	41.0
23	36.37151541143784	38.0	35.0	40.0	30.0	41.0
24	36.54466650506256	38.0	35.0	40.0	30.0	41.0
25	36.503192675604616	38.0	35.0	40.0	30.0	41.0
26	36.291624307743945	38.0	35.0	40.0	30.0	41.0
27	36.21974304946951	38.0	35.0	40.0	30.0	41.0
28	36.012481306755674	38.0	34.0	40.0	29.0	41.0
29	35.81468310056126	38.0	34.0	40.0	28.0	41.0
30	35.60618322176435	38.0	34.0	40.0	27.0	41.0
31	35.30474332916892	38.0	34.0	40.0	27.0	41.0
32	35.145707732756534	38.0	33.0	40.0	26.0	41.0
33	34.92505994890824	38.0	33.0	40.0	25.0	41.0
34	34.788636745044656	37.0	33.0	40.0	25.0	41.0
35	34.50561748307818	37.0	33.0	40.0	24.0	41.0
36	34.359007253538195	37.0	33.0	40.0	24.0	41.0
37	34.155453206287646	37.0	33.0	40.0	23.0	41.0
38	34.060654496634285	37.0	33.0	40.0	23.0	41.0
39	33.93725186000112	37.0	33.0	40.0	22.0	41.0
40	33.791440451994255	37.0	33.0	40.0	21.0	41.0
41	33.549103656603705	37.0	32.0	40.0	19.0	41.0
42	33.27928620708945	37.0	31.0	40.0	18.0	41.0
43	32.94907009267374	37.0	31.0	40.0	16.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	6.0
15	7.0
16	41.0
17	68.0
18	182.0
19	469.0
20	921.0
21	1758.0
22	3368.0
23	5377.0
24	8553.0
25	12592.0
26	17547.0
27	24419.0
28	32105.0
29	40221.0
30	49266.0
31	59232.0
32	70282.0
33	83657.0
34	98976.0
35	117206.0
36	145496.0
37	185221.0
38	222800.0
39	160951.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.55857838109978	22.656100244270824	14.452255309627255	22.333066065002143
2	19.504223461187046	24.70484252922859	34.21186298457924	21.579071025005128
3	20.415745212478324	25.635458427343416	32.74772977307054	21.20106658710772
4	14.375207443733801	16.984989464655317	36.14768129183837	32.49212179977251
5	12.099200059669208	39.540547092058404	34.827723806149656	13.532529042122732
6	31.91243543605139	37.68431259206773	13.362546383486546	17.04070558839434
7	25.484644502041803	33.42728747506013	21.644408808666952	19.443659214231108
8	27.35974938932294	34.406906710921334	18.864494956087192	19.36884894366854
9	24.322139141136326	14.35089224113819	21.99944060116728	39.327528016558205
10	16.200190195603128	27.945775606481565	33.5537116112551	22.3003225866602
11	32.647336329224856	22.52184452441776	23.92399634526096	20.90682280109642
12	20.916295287997166	27.4902011971135	31.31134274366481	20.282160771224525
13	31.76311324097037	20.96276268436853	26.713457271252494	20.560666803408605
14	20.18378116317664	23.07997538645136	28.176844617650897	28.5593988327211
15	22.238490369016763	29.75695985381044	26.16442596356449	21.840123813608308
16	21.407372876615263	28.524417759048276	27.01978407205057	23.04842529228589
17	21.551026496858043	26.61895616177814	27.348859758712635	24.48115758265118
18	22.98487758488877	25.308321989968114	30.096253892483542	21.61054653265957
19	22.462249902105206	24.41760987525406	31.322157787764084	21.797982434876655
20	23.589699602826826	24.245613380820078	31.27531745883757	20.889369557515522
21	22.48708721027802	25.150347759607676	31.78601129985642	20.57655373025788
22	20.85558186801917	25.502992783755058	31.407708515914894	22.233716832310876
23	22.008689328534935	25.97639336925917	32.29252829625762	19.722389005948273
24	22.015849633593763	25.717429002964813	29.600551940181617	22.666169423259802
25	21.845195696358314	26.218202838016747	30.566074325458242	21.3705271401667
26	21.844971936825225	26.813776128587147	30.647522795502436	20.693729139085196
27	20.63122564284249	26.98129743235936	30.230957131402786	22.156519793395365
28	21.111562773872343	26.53504633686998	30.4242853679912	21.92910552126648
29	21.471144343545472	26.227078632829254	29.846538253556844	22.455238770068433
30	21.329206213056366	26.59508847824871	31.54367972552164	20.532025583173283
31	22.20462809300938	26.234164351377053	31.372205336664866	20.189002218948705
32	21.688489436685376	26.000037293255513	30.206119823229972	22.10535344682914
33	21.31906244755636	26.305916574987414	30.78259896697682	21.592422010479403
34	20.68157153778739	27.161125510451434	31.073859292546942	21.083443659214232
35	20.013873091051483	29.444293199574854	29.99779969792463	20.544034011449032
36	22.50051278226333	26.645359786682576	29.943948236961344	20.91017919409275
37	21.388353316302748	27.603423520856254	29.719219079229518	21.28900408361148
38	21.326894031214454	25.85914337392083	31.195286132502936	21.618676462361783
39	20.867739469316973	25.736597736299387	31.84224952917265	21.553413265210985
40	19.653321896734976	26.644912267616398	31.216021182569133	22.48574465307949
41	21.416845363516007	25.41729288258219	31.261220608252998	21.90464114564881
42	19.873948796360178	27.495496839396594	30.64901452572302	21.981539838520202
43	20.380242033228292	27.456786440172294	29.64336459751254	22.519606929086873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	84.0
1	158.5
2	233.0
3	512.5
4	792.0
5	792.0
6	1443.0
7	2094.0
8	2441.5
9	2789.0
10	4323.0
11	5857.0
12	5857.0
13	11449.0
14	17041.0
15	27379.5
16	37718.0
17	34255.5
18	30793.0
19	30793.0
20	33019.0
21	35245.0
22	27872.0
23	20499.0
24	22290.5
25	24082.0
26	24082.0
27	26334.5
28	28587.0
29	31349.5
30	34112.0
31	37155.5
32	40199.0
33	40199.0
34	44723.5
35	49248.0
36	53568.5
37	57889.0
38	61141.0
39	64393.0
40	64393.0
41	66176.5
42	67960.0
43	72222.0
44	76484.0
45	82444.5
46	88405.0
47	88405.0
48	117651.0
49	146897.0
50	137149.5
51	127402.0
52	116882.0
53	106362.0
54	106362.0
55	93251.0
56	80140.0
57	67118.0
58	54096.0
59	47615.0
60	41134.0
61	41134.0
62	36113.0
63	31092.0
64	26941.0
65	22790.0
66	19480.5
67	16171.0
68	16171.0
69	13747.5
70	11324.0
71	9446.0
72	7568.0
73	6168.0
74	4768.0
75	4768.0
76	3845.0
77	2922.0
78	2294.5
79	1667.0
80	1267.0
81	867.0
82	867.0
83	675.5
84	484.0
85	380.0
86	276.0
87	203.5
88	131.0
89	131.0
90	103.5
91	76.0
92	51.5
93	27.0
94	22.5
95	18.0
96	18.0
97	12.5
98	7.0
99	4.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1340725.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.79581134486089
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.68958964284116	46.406347769428095
2	8.941602126256877	9.799246864623576
3	2.4686729990942813	4.0581881979156655
4	1.0445519911007994	2.2894829537703285
5	0.6051451335263338	1.657970928648482
6	0.3904160437280615	1.2835898326877893
7	0.2755726738684888	1.0570159774367613
8	0.19767146433153066	0.8665254614218363
9	0.1572140331139363	0.7753203449348371
>10	1.0530551636202923	10.953032450345798
>50	0.10346229914500087	3.9374720283454576
>100	0.06335242947477128	6.499608621435829
>500	0.0057344788132552196	2.245514698151432
>1k	0.0032768450361458394	3.7523752144261033
>5k	2.7307041967881995E-4	0.8849934485384583
>10k+	4.096056295182299E-4	3.5333152078895744
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	18734	1.3973036976262843	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16053	1.1973372615562476	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12440	0.9278561972067352	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6695	0.4993566913423707	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5134	0.38292714762535196	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4440	0.3311641089708926	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4243	0.31647056629808495	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4034	0.3008819854929236	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3814	0.28447295306643794	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3186	0.23763262413992428	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2372	0.1769192041619273	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2147	0.16013723918029424	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1841	0.13731376680527327	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1841	0.13731376680527327	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1789	0.1334352682317403	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1707	0.1273191743273229	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1702	0.1269462417721755	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1699	0.12672248223908705	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1667	0.1243357138861437	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1617	0.12060638833466969	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1601	0.11941300415819799	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1541	0.11493781349642916	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1418	0.1057636726398031	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1397	0.10419735590818402	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1362	0.10158682802215219	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.458651102948032E-5	0.0
10	2.2375953308844095E-4	0.0	0.0	1.4917302205896065E-4	0.0
11	2.2375953308844095E-4	0.0	0.0	2.2375953308844095E-4	0.0
12	2.2375953308844095E-4	0.0	0.0	3.729325551474016E-4	0.0
13	2.2375953308844095E-4	0.0	0.0	3.729325551474016E-4	0.0
14	2.2375953308844095E-4	0.0	0.0	7.458651102948032E-4	0.0
15	2.2375953308844095E-4	0.0	0.0	8.950381323537638E-4	0.0
16	2.983460441179213E-4	0.0	0.0	0.0019392492867664883	0.0
17	2.983460441179213E-4	0.0	0.0	0.0026851143970612916	0.0
18	3.729325551474016E-4	0.0	0.0	0.003058046952208693	0.0
19	3.729325551474016E-4	0.0	0.0	0.004176844617650898	0.0
20	3.729325551474016E-4	0.0	0.0	0.007234891569859591	0.0
21	3.729325551474016E-4	0.0	0.0	0.014618956161778142	0.0
22	4.475190661768819E-4	0.0	0.0	0.02558317328311175	0.0
23	5.221055772063623E-4	0.0	0.0	0.03140092114341121	0.0
24	5.221055772063623E-4	0.0	0.0	0.041544686643420536	0.0
25	5.221055772063623E-4	0.0	0.0	0.047586194036808444	0.0
26	5.221055772063623E-4	0.0	0.0	0.055492364205933356	0.0
27	5.221055772063623E-4	0.0	0.0	0.09778291595964869	0.0
28	5.221055772063623E-4	0.0	0.0	0.19034477614723377	0.0
29	5.221055772063623E-4	0.0	0.0	0.2963322083201253	0.0
30	5.221055772063623E-4	0.0	0.0	0.4552014768129184	0.0
31	5.966920882358426E-4	0.0	0.0	0.6298830856439613	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5230	0.0	22.921606	1
TAAAGCC	140	3.6379788E-12	19.821428	4
AACGGAC	60	9.237909E-4	18.5	26
ACGGACC	60	9.237909E-4	18.5	27
GACGCAA	50	0.007035477	18.5	10
ACCGTTA	150	2.5102054E-10	17.266666	8
CGGACCA	65	0.0015801196	17.076923	28
GTATTGG	670	0.0	16.014925	1
TAGTACT	220	0.0	15.977273	4
CCGTTTA	140	3.474088E-8	15.857142	27
TTTGCGC	95	7.061077E-5	15.578948	7
GTATCAA	7835	0.0	15.300575	2
TAGGCGA	85	5.364978E-4	15.235294	33
TCGAACC	250	0.0	14.800001	31
TGTTACG	100	1.0935791E-4	14.8	16
GTGCGCC	150	8.103234E-8	14.8	11
CTGTGCG	100	1.0935791E-4	14.8	9
TTATGCG	140	5.9964805E-7	14.535714	4
TCGTTAT	115	2.2102768E-5	14.47826	13
ATTGGAC	800	0.0	14.106251	3
>>END_MODULE