FastQCFastQC Report
Wed 25 May 2016
SRR2088427_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088427_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1349954
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT270372.002808984602438No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT207931.5402747056566373No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT167531.241005249067746No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93450.6922458098572248No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG54090.40068031947755256No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG46870.3471970156020131No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA44320.3283074830697935No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA42790.31697376355046175No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA39900.2955656266806128No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35970.26645352360154495No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28950.21445175168931682No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23260.17230216733310913No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22730.1683761076303341No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC22640.16770941824684396No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA22510.16674642247069157No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21020.1557090093440221No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19220.14237522167422No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18850.13963438754209404No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG18660.13822693217694826No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17820.1320044979310406No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC17790.1317822681365439No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG15650.11592987612911254No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15160.11230012281899975No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT14250.10555915238593316No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT13990.1036331608336284No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13660.10118863309416469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGTA952.8194336E-1023.3684225
TACGTGG400.001931360823.12512
GTATTGG6050.020.4876021
CGGGTAA1001.2902092E-820.3526
GGTATCA76200.019.6653541
TTGGACC10250.018.048784
TTAACGG1156.40739E-817.6956535
GTCGGGT1156.40739E-817.6956524
CTAGGAC953.6073325E-617.5263143
TGTTACG1705.456968E-1217.41176416
ATTGGAC7500.017.2666663
AGAACGA650.00158012717.07692334
TGGACCC10950.016.8949785
ACCGTTA2200.016.8181828
AGTTCCG904.4468812E-516.44444532
TAGCGAA1708.54925E-1116.32352810
GGACCCT11450.015.8340626
GTTCCGA957.0611364E-515.57894633
TAACACT1201.9360104E-615.4166674
CCGTTTA2400.015.41666727
Produced by FastQC (version 0.11.5)