##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088427_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1349954
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.01282339990844	31.0	31.0	33.0	30.0	34.0
2	31.21724962480203	31.0	31.0	34.0	28.0	34.0
3	31.302185111492687	31.0	31.0	34.0	28.0	34.0
4	35.02036958296357	35.0	35.0	37.0	32.0	37.0
5	34.88774136007597	35.0	35.0	37.0	32.0	37.0
6	34.99024855661749	36.0	35.0	37.0	32.0	37.0
7	34.7787191267258	35.0	35.0	37.0	32.0	37.0
8	34.86660434355541	35.0	35.0	37.0	32.0	37.0
9	36.29451447975264	38.0	35.0	39.0	32.0	39.0
10	36.139956620744115	38.0	35.0	39.0	31.0	39.0
11	36.203918800196156	38.0	35.0	39.0	32.0	39.0
12	36.043854086879996	38.0	35.0	39.0	31.0	39.0
13	36.1000885956114	38.0	35.0	39.0	31.0	39.0
14	36.85601213078372	38.0	36.0	40.0	31.0	41.0
15	36.92259217721493	38.0	36.0	40.0	31.0	41.0
16	36.756185766329814	38.0	36.0	40.0	31.0	41.0
17	36.92008764743095	38.0	36.0	40.0	31.0	41.0
18	36.81484109828928	38.0	36.0	40.0	31.0	41.0
19	36.936731177506786	39.0	36.0	40.0	31.0	41.0
20	36.86119749265531	39.0	36.0	40.0	31.0	41.0
21	36.71173239977066	39.0	35.0	40.0	30.0	41.0
22	36.622972338316714	38.0	35.0	40.0	30.0	41.0
23	36.411243642375965	38.0	35.0	40.0	30.0	41.0
24	36.5521765926839	38.0	35.0	40.0	30.0	41.0
25	36.50825509609957	38.0	35.0	40.0	30.0	41.0
26	36.25675393383774	38.0	35.0	40.0	30.0	41.0
27	36.170513958253395	38.0	35.0	40.0	30.0	41.0
28	35.936582283544475	38.0	34.0	40.0	29.0	41.0
29	35.726724021707405	38.0	34.0	40.0	27.0	41.0
30	35.4867388073964	38.0	34.0	40.0	27.0	41.0
31	35.130096284762296	38.0	34.0	40.0	26.0	41.0
32	34.944795155983094	37.0	33.0	40.0	26.0	41.0
33	34.68605819161245	37.0	33.0	40.0	25.0	41.0
34	34.524024522317056	37.0	33.0	40.0	25.0	41.0
35	34.221611995668	37.0	33.0	40.0	24.0	41.0
36	34.0363108668888	37.0	33.0	40.0	23.0	41.0
37	33.804322221349764	37.0	33.0	40.0	22.0	41.0
38	33.648628768091356	37.0	32.0	40.0	21.0	41.0
39	33.47047751256709	37.0	32.0	40.0	20.0	41.0
40	33.271752222668326	36.0	32.0	40.0	18.0	41.0
41	32.96289873580878	36.0	31.0	40.0	16.0	41.0
42	32.62956663708541	36.0	31.0	40.0	15.0	41.0
43	32.246669145763484	36.0	30.0	39.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	8.0
15	15.0
16	34.0
17	83.0
18	207.0
19	525.0
20	1102.0
21	1999.0
22	3683.0
23	5930.0
24	8954.0
25	13922.0
26	19390.0
27	25799.0
28	33908.0
29	42636.0
30	51523.0
31	61758.0
32	72868.0
33	86182.0
34	101830.0
35	119850.0
36	148113.0
37	188291.0
38	211981.0
39	149359.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.009101050850624	22.61247420282469	15.205036616062472	21.173388130262218
2	19.58977861467872	25.58131610410429	33.64351674205195	21.185388539165036
3	21.129979243737193	25.425681171358434	33.18609374837958	20.2582458365248
4	14.541310296498992	16.914354118732934	35.240089662314425	33.30424592245365
5	12.071374283864488	40.20322173940742	33.8961920183947	13.829211958333396
6	31.963533572255052	37.25512128561418	12.812658801707316	17.96868634042345
7	24.95996159869151	34.005677230483414	21.13657206097393	19.89778910985115
8	28.323113232006424	33.807300100596024	18.451739837061115	19.41784683033644
9	24.089931953236928	14.499012558946452	22.57054684826298	38.84050863955365
10	16.885612398644696	28.467118138840284	32.332212801325085	22.315056661189935
11	32.19428217554079	22.917817940463156	23.60695253319743	21.28094735079862
12	21.036346423655917	28.420227652201486	30.914534865632458	19.628891058510142
13	32.62074115117996	20.783893377107667	26.866396929080544	19.728968542631822
14	19.975273231532334	23.803848131121505	28.992469373030488	27.228409264315673
15	22.071492806421553	30.12895254208662	26.708836004782384	21.090718646709444
16	20.45543774084154	28.641790757314695	27.743167544968202	23.159603956875568
17	21.124127192482113	26.776690168702043	28.083919896529807	24.015262742286033
18	22.511285569730525	25.008704000284453	31.087133339358232	21.39287709062679
19	21.997638438050483	24.40586864441307	32.8723793551484	20.72411356238805
20	22.935374094228393	24.19793563336232	32.603851686798215	20.262838585611064
21	21.61369942975835	25.31019575481831	33.32639482530516	19.74970999011818
22	19.977643682673634	25.84858447028565	32.72267055025579	21.451101296784927
23	21.21724147637623	26.1421500288158	33.68225880289255	18.958349691915426
24	21.127534715997733	26.11896405358997	30.920535070083872	21.832966160328425
25	20.901675168190916	26.649278419857268	31.980052653645973	20.46899375830584
26	20.845228800388753	27.37071040939173	32.09864928730905	19.68541150291047
27	19.491182662520355	27.453676199337163	31.755970944195134	21.29917019394735
28	20.010311462464646	27.135665363412382	31.86434500731136	20.989678166811608
29	20.31787749804808	26.890323670288023	31.131801528052065	21.659997303611828
30	20.2803947393763	27.194259952561346	33.11068377144702	19.41466153661532
31	21.117608451843545	26.96995601331601	32.727855912127374	19.184579622713073
32	20.714705834421025	26.825951106482147	31.3879584045086	21.07138465458823
33	20.270024015633126	26.982400881807823	31.96975600650096	20.777819096058085
34	19.316806350438608	27.965767722455727	32.43147544286694	20.28595048423872
35	18.86027227594422	30.299032411474762	31.1923961853515	19.64829912722952
36	21.599995259097717	27.432564368859975	31.01164928582752	19.955791086214788
37	20.43491852314968	28.45778448747143	30.7814932953271	20.325803694051796
38	20.196021494065725	26.608388137669873	32.36658434287391	20.829006025390495
39	19.69793044800045	26.47312426941955	33.09431284325244	20.734632439327562
40	18.451443530668453	27.457157799450943	32.36302866616196	21.728370003718645
41	20.303358484807628	26.217189622757513	32.25391383706408	21.225538055370777
42	18.86123527172037	28.402893728230737	31.56122356761786	21.174647432431033
43	19.334510657400177	28.168885754625713	30.494668707230023	22.001934880744084
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	236.0
2	350.0
3	756.5
4	1163.0
5	1163.0
6	2053.0
7	2943.0
8	3434.0
9	3925.0
10	6040.0
11	8155.0
12	8155.0
13	15810.0
14	23465.0
15	38788.5
16	54112.0
17	47975.5
18	41839.0
19	41839.0
20	43565.5
21	45292.0
22	34260.5
23	23229.0
24	24433.5
25	25638.0
26	25638.0
27	27205.0
28	28772.0
29	30469.0
30	32166.0
31	34628.5
32	37091.0
33	37091.0
34	40350.0
35	43609.0
36	47821.5
37	52034.0
38	55170.5
39	58307.0
40	58307.0
41	61086.5
42	63866.0
43	68088.5
44	72311.0
45	79766.0
46	87221.0
47	87221.0
48	120696.0
49	154171.0
50	142271.5
51	130372.0
52	117984.5
53	105597.0
54	105597.0
55	90921.5
56	76246.0
57	63510.0
58	50774.0
59	43928.5
60	37083.0
61	37083.0
62	32442.5
63	27802.0
64	23970.0
65	20138.0
66	17271.0
67	14404.0
68	14404.0
69	12367.0
70	10330.0
71	8620.5
72	6911.0
73	5722.5
74	4534.0
75	4534.0
76	3594.0
77	2654.0
78	2097.5
79	1541.0
80	1172.0
81	803.0
82	803.0
83	646.5
84	490.0
85	368.0
86	246.0
87	188.0
88	130.0
89	130.0
90	98.5
91	67.0
92	50.0
93	33.0
94	24.5
95	16.0
96	16.0
97	8.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1349954.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.843705926480716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.56702286296097	41.652901127329166
2	9.261211275631387	9.232261826911563
3	2.6826528853007634	4.0114008455326875
4	1.1826846850438661	2.357975505803157
5	0.6637525591303634	1.6541943682621418
6	0.4422805173925817	1.3226940027556544
7	0.30689468676404497	1.070773796222653
8	0.22886788491469517	0.9126098841362957
9	0.18331856283344872	0.8223548883061945
>10	1.2656394963721305	12.241188998917114
>50	0.12150647759703599	4.2510871907577386
>100	0.08254610002420765	7.5228950893646145
>500	0.0056620029399195755	2.0326179127875226
>1k	0.005215002707820662	5.022910858838777
>5k	2.9800015473260925E-4	1.0957376844337428
>10k+	4.470002320989139E-4	4.796396019641074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27037	2.002808984602438	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20793	1.5402747056566373	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	16753	1.241005249067746	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9345	0.6922458098572248	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5409	0.40068031947755256	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4687	0.3471970156020131	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4432	0.3283074830697935	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4279	0.31697376355046175	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3990	0.2955656266806128	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3597	0.26645352360154495	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2895	0.21445175168931682	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2326	0.17230216733310913	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2273	0.1683761076303341	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2264	0.16770941824684396	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2251	0.16674642247069157	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2102	0.1557090093440221	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1922	0.14237522167422	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1885	0.13963438754209404	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1866	0.13822693217694826	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1782	0.1320044979310406	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1779	0.1317822681365439	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1565	0.11592987612911254	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1516	0.11230012281899975	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1425	0.10555915238593316	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1399	0.1036331608336284	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1366	0.10118863309416469	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.407659816556713E-5	0.0
5	0.0	0.0	0.0	7.407659816556713E-5	0.0
6	0.0	0.0	0.0	7.407659816556713E-5	0.0
7	0.0	0.0	0.0	7.407659816556713E-5	0.0
8	0.0	0.0	0.0	7.407659816556713E-5	0.0
9	0.0	0.0	0.0	7.407659816556713E-5	0.0
10	0.0	0.0	0.0	2.2222979449670137E-4	0.0
11	0.0	0.0	0.0	6.666893834901041E-4	0.0
12	0.0	0.0	0.0	8.148425798212383E-4	0.0
13	0.0	0.0	0.0	8.889191779868055E-4	0.0
14	0.0	0.0	0.0	0.001185225570649074	0.0
15	0.0	0.0	0.0	0.0014074553651457753	0.0
16	0.0	0.0	0.0	0.0021482213468014466	0.0
17	7.407659816556713E-5	0.0	0.0	0.0033334469174505204	0.0
18	7.407659816556713E-5	0.0	0.0	0.0037779065064439234	0.0
19	1.4815319633113425E-4	0.0	0.0	0.005037208675258564	7.407659816556713E-5
20	1.4815319633113425E-4	0.0	0.0	0.008815115181702488	7.407659816556713E-5
21	1.4815319633113425E-4	0.0	0.0	0.014296783445954454	7.407659816556713E-5
22	1.4815319633113425E-4	0.0	0.0	0.024297124198306017	7.407659816556713E-5
23	1.4815319633113425E-4	0.0	0.0	0.031852937211193866	7.407659816556713E-5
24	2.2222979449670137E-4	0.0	0.0	0.04192735456171099	7.407659816556713E-5
25	2.2222979449670137E-4	0.0	0.0	0.04918686118193657	7.407659816556713E-5
26	2.2222979449670137E-4	0.0	0.0	0.06074281049576504	7.407659816556713E-5
27	2.2222979449670137E-4	0.0	0.0	0.11578172293278141	7.407659816556713E-5
28	2.2222979449670137E-4	0.0	0.0	0.2288966883316024	7.407659816556713E-5
29	2.2222979449670137E-4	0.0	0.0	0.3846056976756245	7.407659816556713E-5
30	2.2222979449670137E-4	0.0	0.0	0.583353210553841	1.4815319633113425E-4
31	2.2222979449670137E-4	0.0	0.0	0.7985457282248136	1.4815319633113425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGGGTA	95	2.8194336E-10	23.36842	25
TACGTGG	40	0.0019313608	23.125	12
GTATTGG	605	0.0	20.487602	1
CGGGTAA	100	1.2902092E-8	20.35	26
GGTATCA	7620	0.0	19.665354	1
TTGGACC	1025	0.0	18.04878	4
TTAACGG	115	6.40739E-8	17.69565	35
GTCGGGT	115	6.40739E-8	17.69565	24
CTAGGAC	95	3.6073325E-6	17.526314	3
TGTTACG	170	5.456968E-12	17.411764	16
ATTGGAC	750	0.0	17.266666	3
AGAACGA	65	0.001580127	17.076923	34
TGGACCC	1095	0.0	16.894978	5
ACCGTTA	220	0.0	16.818182	8
AGTTCCG	90	4.4468812E-5	16.444445	32
TAGCGAA	170	8.54925E-11	16.323528	10
GGACCCT	1145	0.0	15.834062	6
GTTCCGA	95	7.0611364E-5	15.578946	33
TAACACT	120	1.9360104E-6	15.416667	4
CCGTTTA	240	0.0	15.416667	27
>>END_MODULE