FastQCFastQC Report
Wed 25 May 2016
SRR2088426_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088426_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3385430
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT256620.7580130145948963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT247730.7317534257095849No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT193020.570149139104929No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCAC172100.5083549209406191No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGC139690.4126211441382631No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTA124680.36828408798882273No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122740.3625536490194746No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCT104470.3085870923339133No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAG79090.23361877220914332No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATT77380.22856771517946023No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGA76300.22537757389755514No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA68610.20266258643658266No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACC64910.19173339871153738No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGAC52980.1564941528845671No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACC51740.1528313980794168No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAG49150.1451809666718851No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAA48760.14402897120897493No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCT41460.12246597921091265No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTC40730.12030968001110642No Hit
CTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCC39480.11661738686075329No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTC35270.10418174353036395No Hit
ATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA34440.10173006087852945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.027.4824941
GTATCAA105400.017.1835862
TCCATCA22100.015.9886882
CCATCAC22750.015.5318683
ATCACTG24000.015.26255
GTACCGA1101.4534491E-515.1363646
GTATTAG6300.014.682541
ACCGCCT4500.014.38888912
CATCACT25500.014.2921574
CGACTCT55700.014.2154427
CTCTTCG55150.014.15593930
TCGACTC57050.014.04119226
CTCGACT57550.013.79061725
ACTGTAC41250.013.7684853
CACTGTA40800.013.5575982
TCTTCGA57950.013.53580731
GACTCTT58250.013.49785328
CGAAGGC55800.013.4274235
TCGAAGG56650.013.32391834
TGCATTC63300.013.2977910
Produced by FastQC (version 0.11.5)