##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088426_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3385430
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26473771426377	31.0	31.0	34.0	30.0	34.0
2	31.488702764493727	31.0	31.0	34.0	30.0	34.0
3	31.613311455265652	31.0	31.0	34.0	30.0	34.0
4	35.3199815089959	37.0	35.0	37.0	33.0	37.0
5	35.17206706385895	37.0	35.0	37.0	32.0	37.0
6	35.21514490035239	37.0	35.0	37.0	32.0	37.0
7	35.07772661079981	37.0	35.0	37.0	32.0	37.0
8	35.10034796170649	37.0	35.0	37.0	32.0	37.0
9	36.64624641478335	39.0	35.0	39.0	32.0	39.0
10	36.465463176021956	38.0	35.0	39.0	32.0	39.0
11	36.538305621442476	38.0	35.0	39.0	32.0	39.0
12	36.37613035862505	38.0	35.0	39.0	32.0	39.0
13	36.361889627019316	38.0	35.0	39.0	32.0	39.0
14	37.363006767234886	39.0	36.0	41.0	32.0	41.0
15	37.40085543047707	39.0	36.0	41.0	32.0	41.0
16	37.242637124383016	39.0	36.0	41.0	31.0	41.0
17	37.3170542589863	39.0	36.0	41.0	32.0	41.0
18	37.179352696703226	39.0	36.0	40.0	31.0	41.0
19	37.280412532529105	39.0	36.0	40.0	31.0	41.0
20	37.19919035395799	39.0	36.0	40.0	31.0	41.0
21	37.03408843189787	39.0	36.0	40.0	31.0	41.0
22	36.93785634321194	39.0	36.0	40.0	31.0	41.0
23	36.785778172935196	39.0	35.0	40.0	30.0	41.0
24	36.961818144223926	39.0	36.0	40.0	31.0	41.0
25	36.95048900730483	39.0	36.0	40.0	31.0	41.0
26	36.77876872361857	39.0	36.0	40.0	30.0	41.0
27	36.72798196979409	39.0	35.0	40.0	30.0	41.0
28	36.59208017888422	39.0	35.0	40.0	30.0	41.0
29	36.43421160679736	39.0	35.0	40.0	30.0	41.0
30	36.32646517576792	38.0	35.0	40.0	30.0	41.0
31	36.162342745234724	38.0	35.0	40.0	29.0	41.0
32	36.01981077736063	38.0	35.0	40.0	29.0	41.0
33	35.8827203634398	38.0	35.0	40.0	29.0	41.0
34	35.774672936672744	38.0	34.0	40.0	28.0	41.0
35	35.55853702483879	38.0	34.0	40.0	27.0	41.0
36	35.477523977751716	38.0	34.0	40.0	27.0	41.0
37	35.30388222471001	38.0	34.0	40.0	26.0	41.0
38	35.33831064296116	38.0	34.0	40.0	26.0	41.0
39	35.2682436204559	38.0	34.0	40.0	25.0	41.0
40	35.212495015404244	38.0	34.0	40.0	25.0	41.0
41	35.04966252440606	38.0	34.0	40.0	25.0	41.0
42	34.914630637762414	38.0	33.0	40.0	24.0	41.0
43	34.6466832869089	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	1.0
14	6.0
15	18.0
16	56.0
17	108.0
18	248.0
19	581.0
20	1336.0
21	2678.0
22	4865.0
23	8530.0
24	13946.0
25	21578.0
26	31487.0
27	44086.0
28	60928.0
29	80202.0
30	103109.0
31	127668.0
32	156185.0
33	189761.0
34	230520.0
35	280828.0
36	351037.0
37	457880.0
38	616200.0
39	601586.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.635437743506735	23.22582360291012	13.990394130140041	23.148344523443107
2	17.32403269304047	23.934596196051906	36.71545416682667	22.025916944080958
3	18.564259193071486	27.766753410940414	32.420992311168746	21.247995084819358
4	12.989605456323124	17.814900913621017	39.19605485861471	29.999438771441145
5	12.454429717938343	39.36141051506012	35.43112691740784	12.753032849593701
6	30.010840572689435	39.38353473561704	16.088857250039137	14.516767441654382
7	24.846740295915144	33.52667164880089	24.47804267109348	17.148545384190488
8	25.64938575011151	37.090207152414905	20.62517907621779	16.635228021255795
9	24.7448920816558	14.469594704365472	22.758910980289063	38.026602233689665
10	15.197242300091865	28.116841878284294	36.481835394617526	20.204080427006318
11	33.08460077449541	23.473709395852225	24.128958507486495	19.31273132216587
12	19.555772826494714	27.58562427815669	31.82233866894309	21.036264226405507
13	29.098785087861806	22.748572559468073	27.555613319430616	20.5970290332395
14	20.424790942361824	22.691829398333446	29.582593643938882	27.300786015365848
15	22.821177811976618	29.72322570544953	26.732320561937478	20.723275920636375
16	20.269507861630576	29.621347952844985	28.04246432506358	22.06667986046086
17	20.49760296328679	28.394620476571664	28.337670547020615	22.770106013120934
18	21.20705493836824	27.851498923327316	29.817659795062962	21.12378634324148
19	22.495547094460676	27.56107791329314	30.37608221112237	19.567292781123815
20	21.570612891124615	27.774285688967133	30.006350744218608	20.648750675689648
21	21.82727157259196	26.376590270659854	31.448412757020527	20.347725399727658
22	21.034167003896105	26.944110497041734	29.954865408530086	22.066857090532075
23	21.53566902874967	27.822196884886115	30.990125331198698	19.652008755165518
24	21.37190844294521	28.619436821910366	29.349713330359805	20.65894140478462
25	20.61543142230086	27.601397754495	30.722685153732314	21.060485669471827
26	20.529474837760638	26.700921300986874	31.031951628005892	21.73765223324659
27	20.986551191429154	27.25665572763283	30.502329098519244	21.254463982418777
28	21.283322945681938	27.5177746992258	29.799139252620787	21.39976310247147
29	20.870908569960093	28.643097036417824	29.88485953039939	20.601134863222693
30	20.364946254980904	28.60056181932576	29.17372386964138	21.860768056051963
31	21.822752205775927	27.773813075443886	30.197345684300075	20.206089034480108
32	19.62619224145825	27.608280188927253	30.69347173032672	22.07205583928777
33	19.89324842043699	28.057144882629387	31.905134650546607	20.14447204638702
34	20.505164779658713	27.92283402699214	31.426140844737592	20.14586034861155
35	20.334698989493212	28.59985289904089	30.126719500920117	20.93872861054578
36	21.943150500822643	28.472660784597526	29.63168637366597	19.95250234091386
37	19.615558437185232	28.95065028666964	30.27591768253959	21.15787359360554
38	20.96599250316799	28.599262132136833	30.296417294110352	20.13832807058483
39	20.72061746956812	27.1737711309937	31.150193623852807	20.955417775585374
40	20.297185291085622	27.871791766481657	31.33598981517857	20.495033127254146
41	21.362544787515915	26.833164472459924	30.317773517691993	21.48651722233217
42	19.47802790192088	28.347802199425182	30.63607872559763	21.538091173056305
43	19.864537148899846	27.067521703299136	30.129053030191145	22.938888117609878
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	710.5
2	1120.0
3	2272.5
4	3425.0
5	3425.0
6	5364.5
7	7304.0
8	8362.5
9	9421.0
10	13944.0
11	18467.0
12	18467.0
13	31116.5
14	43766.0
15	57633.0
16	71500.0
17	69430.0
18	67360.0
19	67360.0
20	74280.0
21	81200.0
22	72069.0
23	62938.0
24	69169.5
25	75401.0
26	75401.0
27	83176.5
28	90952.0
29	98238.5
30	105525.0
31	115288.0
32	125051.0
33	125051.0
34	135168.5
35	145286.0
36	165930.0
37	186574.0
38	209853.0
39	233132.0
40	233132.0
41	237394.0
42	241656.0
43	256893.5
44	272131.0
45	257215.0
46	242299.0
47	242299.0
48	256326.5
49	270354.0
50	249272.0
51	228190.0
52	215889.5
53	203589.0
54	203589.0
55	188429.0
56	173269.0
57	150978.5
58	128688.0
59	111403.5
60	94119.0
61	94119.0
62	80523.0
63	66927.0
64	56557.0
65	46187.0
66	38921.5
67	31656.0
68	31656.0
69	26826.5
70	21997.0
71	18331.0
72	14665.0
73	11854.0
74	9043.0
75	9043.0
76	7252.0
77	5461.0
78	4277.5
79	3094.0
80	2354.5
81	1615.0
82	1615.0
83	1223.5
84	832.0
85	641.5
86	451.0
87	328.5
88	206.0
89	206.0
90	192.5
91	179.0
92	119.5
93	60.0
94	46.0
95	32.0
96	32.0
97	19.0
98	6.0
99	3.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3385430.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.24301596974043
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3397492868383	41.16851055710398
2	11.452682307735532	11.737399647833112
3	3.508635700092122	5.39379225635466
4	1.489008007316982	3.0520504439206193
5	0.7766305423439681	1.9898445641960065
6	0.4927915209343939	1.515127426619629
7	0.31551612334025336	1.1317598422924637
8	0.24312121353821736	0.9966611382337237
9	0.17204164516484585	0.7934339493578352
>10	1.0598349929540796	10.063007744505546
>50	0.08227896073839475	2.9198636986702
>100	0.05550547009812389	5.695393740033547
>500	0.005610474899910834	2.011327665762452
>1k	0.005726154793753574	6.101796876322656
>5k	4.0487963188156587E-4	1.396023406267388
>10k+	4.627195792932181E-4	4.034007042526121
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	25662	0.7580130145948963	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	24773	0.7317534257095849	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	19302	0.570149139104929	No Hit
ACTTAGAGCTGCATTCCCAAACAACTCGACTCTTCGAAGGCAC	17210	0.5083549209406191	No Hit
GCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGGC	13969	0.4126211441382631	No Hit
CTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTA	12468	0.36828408798882273	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12274	0.3625536490194746	No Hit
TCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCT	10447	0.3085870923339133	No Hit
CGCCAATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAG	7909	0.23361877220914332	No Hit
TTCCATCACTGTACTTGTTCGCTATCGGTCTCTCGCCAATATT	7738	0.22856771517946023	No Hit
GTACTTGTTCGCTATCGGTCTCTCGCCAATATTTAGCTTTAGA	7630	0.22537757389755514	No Hit
ATATTTAGCTTTAGATGGAATTTACCACCCACTTAGAGCTGCA	6861	0.20266258643658266	No Hit
CTATCGGTCTCTCGCCAATATTTAGCTTTAGATGGAATTTACC	6491	0.19173339871153738	No Hit
GAATTTACCACCCACTTAGAGCTGCATTCCCAAACAACTCGAC	5298	0.1564941528845671	No Hit
TTCCCAAACAACTCGACTCTTCGAAGGCACTTTACAAAGAACC	5174	0.1528313980794168	No Hit
GGTAAATTCCATCTAAAGCTAAATATTGGCGAGAGACCGATAG	4915	0.1451809666718851	No Hit
GTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAA	4876	0.14402897120897493	No Hit
GTCATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTGCGGTTCT	4146	0.12246597921091265	No Hit
CTTTGGGGCCGTTCCTTGTCTATGTTCCTTGGAACAGGACGTC	4073	0.12030968001110642	No Hit
CTTTACAAAGAACCGCACTCCTCGCCACACGGGATTCTCACCC	3948	0.11661738686075329	No Hit
AATTTGGAGAGGGCAACTTTGGGGCCGTTCCTTGTCTATGTTC	3527	0.10418174353036395	No Hit
ATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATA	3444	0.10173006087852945	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	2.9538345202825046E-5	0.0	0.0	0.0	0.0
9	2.9538345202825046E-5	0.0	0.0	2.9538345202825046E-5	0.0
10	2.9538345202825046E-5	0.0	0.0	2.9538345202825046E-5	0.0
11	2.9538345202825046E-5	0.0	0.0	5.907669040565009E-5	0.0
12	2.9538345202825046E-5	0.0	0.0	1.1815338081130018E-4	0.0
13	2.9538345202825046E-5	0.0	0.0	1.4769172601412524E-4	0.0
14	5.907669040565009E-5	0.0	0.0	1.4769172601412524E-4	0.0
15	5.907669040565009E-5	0.0	0.0	2.9538345202825047E-4	0.0
16	5.907669040565009E-5	2.9538345202825046E-5	0.0	4.135368328395507E-4	0.0
17	5.907669040565009E-5	2.9538345202825046E-5	0.0	7.384586300706262E-4	0.0
18	5.907669040565009E-5	2.9538345202825046E-5	0.0	8.861503560847515E-4	0.0
19	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.0010338420820988766	0.0
20	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.001890454092980803	0.0
21	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.003662754805150306	0.0
22	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.005435055517319809	0.0
23	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.006911972777461061	0.0
24	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.00959996219091814	0.0
25	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.012022106497549794	0.0
26	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.015300862815063375	0.0
27	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.036066319492649386	0.0
28	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.08010799219006152	0.0
29	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.13537423606454718	0.0
30	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.20289889319820525	0.0
31	5.907669040565009E-5	2.9538345202825046E-5	0.0	0.29733298281163695	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6570	0.0	27.482494	1
GTATCAA	10540	0.0	17.183586	2
TCCATCA	2210	0.0	15.988688	2
CCATCAC	2275	0.0	15.531868	3
ATCACTG	2400	0.0	15.2625	5
GTACCGA	110	1.4534491E-5	15.136364	6
GTATTAG	630	0.0	14.68254	1
ACCGCCT	450	0.0	14.388889	12
CATCACT	2550	0.0	14.292157	4
CGACTCT	5570	0.0	14.21544	27
CTCTTCG	5515	0.0	14.155939	30
TCGACTC	5705	0.0	14.041192	26
CTCGACT	5755	0.0	13.790617	25
ACTGTAC	4125	0.0	13.768485	3
CACTGTA	4080	0.0	13.557598	2
TCTTCGA	5795	0.0	13.535807	31
GACTCTT	5825	0.0	13.497853	28
CGAAGGC	5580	0.0	13.42742	35
TCGAAGG	5665	0.0	13.323918	34
TGCATTC	6330	0.0	13.29779	10
>>END_MODULE