FastQCFastQC Report
Wed 25 May 2016
SRR2088425_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088425_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315552
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT221641.6847680669407215No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196811.4960260027729804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120840.91854977986427No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81850.622172289654837No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG52080.3958794483228333No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG48150.3660060567731264No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA42700.3245785799421079No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA38380.29174065335311716No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34300.2607270560190703No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG32440.24658850429325482No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25310.19239072267762886No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21270.161681180219406No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC18150.13796489990513489No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17260.13119967891805112No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17260.13119967891805112No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16960.12891926734937123No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG16620.12633480090486732No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA14640.11128408455157988No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT13630.10360669893702415No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA13590.10330264406120016No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58700.024.362011
TATACCG400.001931335223.1255
GCGTGTC450.00382609720.55555711
TAGACTC500.00703540918.55
GTATCAA84750.016.8519172
GTATTGG5300.016.7547171
CGAACTA2650.016.05660424
TACCCGG700.002592760815.8571425
TCCTCGA700.002592760815.85714223
TAACCCG700.002592760815.8571425
TACACAG2350.015.7446815
GACGTAT1455.3467375E-815.31034528
TTAACGG1601.0966687E-815.0312535
TTTACAC1853.0559022E-1015.03
ACGTATA1508.102688E-814.829
TTTTTAC8700.014.6724131
GGTAAGA2400.014.6458332
GGGTAAG2059.276846E-1114.4390241
TCCGACG908.275133E-414.38888923
GGTTACA908.275133E-414.3888891
Produced by FastQC (version 0.11.5)