##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088425_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1315552
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.654219673566686	31.0	30.0	31.0	28.0	34.0
2	30.87347896548369	31.0	31.0	33.0	27.0	34.0
3	30.89693907956508	31.0	31.0	34.0	27.0	34.0
4	34.641143793631876	35.0	35.0	37.0	32.0	37.0
5	34.440649248376346	35.0	35.0	37.0	30.0	37.0
6	34.64546745396609	35.0	35.0	37.0	31.0	37.0
7	34.45307597115127	35.0	35.0	37.0	30.0	37.0
8	34.572360499622974	35.0	35.0	37.0	30.0	37.0
9	35.95990884434823	37.0	35.0	39.0	30.0	39.0
10	35.71426443044441	37.0	35.0	39.0	30.0	39.0
11	35.84227609398944	37.0	35.0	39.0	30.0	39.0
12	35.62774105470555	37.0	35.0	39.0	30.0	39.0
13	35.727319786675096	37.0	35.0	39.0	30.0	39.0
14	36.430824475201284	38.0	35.0	40.0	30.0	41.0
15	36.50142525723042	38.0	35.0	40.0	30.0	41.0
16	36.294371488166185	38.0	35.0	40.0	30.0	41.0
17	36.4505333122522	38.0	35.0	40.0	30.0	41.0
18	36.319473498577025	38.0	35.0	40.0	30.0	41.0
19	36.44929656904478	38.0	35.0	40.0	30.0	41.0
20	36.35282603804335	38.0	35.0	40.0	30.0	41.0
21	36.18355184743743	38.0	34.0	40.0	29.0	41.0
22	36.08198839726594	38.0	34.0	40.0	29.0	41.0
23	35.8553192880251	38.0	34.0	40.0	27.0	41.0
24	36.01558205224879	38.0	34.0	40.0	29.0	41.0
25	35.98086734693877	38.0	34.0	40.0	29.0	41.0
26	35.71198097832697	38.0	34.0	40.0	27.0	41.0
27	35.62985195568096	38.0	34.0	40.0	27.0	41.0
28	35.38646514923013	38.0	34.0	40.0	27.0	41.0
29	35.20615756731775	38.0	33.0	40.0	27.0	41.0
30	34.98951162705845	37.0	33.0	40.0	26.0	41.0
31	34.663511590571865	37.0	33.0	40.0	25.0	41.0
32	34.47594393836199	37.0	33.0	40.0	25.0	41.0
33	34.23562504560823	37.0	33.0	40.0	24.0	41.0
34	34.084500650677434	37.0	33.0	40.0	24.0	41.0
35	33.77451214395174	36.0	32.0	39.0	23.0	40.0
36	33.635838796185936	36.0	32.0	39.0	22.0	40.0
37	33.42385857799616	36.0	31.0	39.0	21.0	40.0
38	33.290009820972486	36.0	31.0	39.0	21.0	40.0
39	33.136459068132616	36.0	31.0	39.0	19.0	40.0
40	32.977070461676924	36.0	31.0	39.0	18.0	40.0
41	32.70406719004646	36.0	31.0	39.0	16.0	40.0
42	32.41847300600813	36.0	30.0	39.0	15.0	40.0
43	32.0566317408966	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	6.0
14	11.0
15	25.0
16	72.0
17	123.0
18	314.0
19	719.0
20	1378.0
21	2579.0
22	4497.0
23	7150.0
24	10794.0
25	15754.0
26	21328.0
27	28529.0
28	36933.0
29	45664.0
30	55338.0
31	65705.0
32	77021.0
33	91008.0
34	106683.0
35	126245.0
36	155222.0
37	187000.0
38	194800.0
39	80650.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.800214662742334	24.926722774926418	15.744797621074651	20.528264941256598
2	21.902440952543113	25.820188027535206	31.691563693415386	20.585807326506288
3	22.841134367930724	25.496597625939533	32.20952117438155	19.452746831748193
4	16.83559448809321	18.57509243268225	33.67993055386636	30.909382525358176
5	11.97641750383109	41.0021040597407	33.90189061321787	13.119587823210333
6	32.06524713580307	37.947036681180215	12.803978862105033	17.18373732091168
7	24.872448979591837	34.079154605823256	21.337278952105276	19.711117462479628
8	27.87461080975894	34.936057259614216	18.393267616939504	18.796064313687335
9	24.320133297657563	14.025215270852085	21.926157232857385	39.728494198632966
10	16.65407372722629	28.889774026416287	32.89463282333195	21.56151942302547
11	33.270444649850404	22.443886672666682	23.29600046216341	20.9896682153195
12	20.336102259735835	28.46972221546545	31.274552431222784	19.91962309357593
13	32.45124480066162	20.698915740312813	26.87024154119336	19.97959791783221
14	19.862384763202062	23.25708143805794	28.9153146359855	27.965219162754494
15	22.63703753253387	29.929641701734326	26.312528885213204	21.120791880518595
16	20.837412736250638	28.24487363479361	28.061300503514875	22.85641312544088
17	21.611156381503736	26.834743134440902	28.087677263992607	23.466423220062758
18	22.422906886234827	25.14267775048041	30.63436489017538	21.800050473109387
19	22.48348982024276	24.237506385152393	32.30871907761913	20.97028471698572
20	23.0664390309163	24.01706660018	32.29161599085403	20.624878378049672
21	22.03660516650045	24.935920434920096	33.0866434774148	19.94083092116465
22	20.52461628274671	25.314392741602003	32.49480066162341	21.666190314027876
23	21.651519666269365	25.620499987837803	33.40483690496461	19.32314344092822
24	21.52237235776313	25.697273843983364	30.75089392133492	22.029459876918587
25	21.560379217241127	26.065788353482038	31.60969691809978	20.764135511177056
26	21.269094646201747	26.78715854637445	31.793422076816423	20.15032473060738
27	20.4382646980127	26.640680109946242	31.35809150835543	21.56296368368563
28	20.658020359514484	26.618788158886915	31.66792342682007	21.055268054778526
29	21.083393130792246	25.82186032935224	31.0205905961908	22.074155943664714
30	21.082252925007904	26.200256622315194	32.54960655299068	20.167883899686213
31	21.688842402276762	26.170611271922358	32.20191980248595	19.93862652331493
32	21.41526902775413	25.933448468779645	31.260717934372796	21.390564569093428
33	21.050023108170564	26.188246454720147	31.678261292598087	21.083469144511202
34	20.348644523363575	26.92824000875678	32.13419157889616	20.588923888983484
35	19.809707255965556	28.825618447617423	31.141072340736052	20.223601955680962
36	22.37653851767167	26.327275546690664	30.838157670696408	20.458028264941255
37	21.21474483714821	27.24780168324779	30.646831140084164	20.890622339519837
38	21.194449174186957	25.56774646688234	31.982544209578943	21.255260149351756
39	20.451339057673128	25.579148524725742	32.6639311863005	21.305581231300625
40	19.519562890710514	26.426777504804065	31.784300430541705	22.269359173943716
41	21.109845904988934	25.374975675609935	31.80353190143757	21.711646517963562
42	20.114446255260148	27.277218992483764	30.889238889834836	21.719095862421252
43	20.632327722507355	27.015579771837224	30.118231738464157	22.233860767191263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	94.0
1	156.5
2	219.0
3	401.0
4	583.0
5	583.0
6	1181.5
7	1780.0
8	2274.0
9	2768.0
10	4319.5
11	5871.0
12	5871.0
13	12566.0
14	19261.0
15	31528.0
16	43795.0
17	38075.5
18	32356.0
19	32356.0
20	36418.0
21	40480.0
22	31013.0
23	21546.0
24	22384.5
25	23223.0
26	23223.0
27	25290.5
28	27358.0
29	29736.5
30	32115.0
31	35304.0
32	38493.0
33	38493.0
34	42978.0
35	47463.0
36	52036.5
37	56610.0
38	60331.0
39	64052.0
40	64052.0
41	66642.0
42	69232.0
43	72945.5
44	76659.0
45	82913.5
46	89168.0
47	89168.0
48	116702.5
49	144237.0
50	133309.0
51	122381.0
52	110396.0
53	98411.0
54	98411.0
55	85868.5
56	73326.0
57	61830.5
58	50335.0
59	44360.5
60	38386.0
61	38386.0
62	33901.5
63	29417.0
64	25552.0
65	21687.0
66	18493.5
67	15300.0
68	15300.0
69	13081.0
70	10862.0
71	8982.5
72	7103.0
73	5959.5
74	4816.0
75	4816.0
76	3817.5
77	2819.0
78	2198.5
79	1578.0
80	1220.0
81	862.0
82	862.0
83	650.5
84	439.0
85	336.0
86	233.0
87	176.5
88	120.0
89	120.0
90	96.5
91	73.0
92	53.0
93	33.0
94	19.0
95	5.0
96	5.0
97	3.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1315552.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.68370243596593
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66154371808005	45.4494512073093
2	8.696752215019876	9.337477161409092
3	2.4975249193704743	4.02229153793683
4	1.12742742205196	2.4209791297434236
5	0.6236288988875994	1.6739354119175487
6	0.42453397967973183	1.3674333503449878
7	0.28922464385669566	1.086865480256575
8	0.22291936401543183	0.9573709444015372
9	0.1783900762384464	0.8618975793282629
>10	1.0996779731545665	11.19656620659411
>50	0.10151582989015631	3.815439012714345
>100	0.06691592780928605	6.778222409059727
>500	0.005540803512604234	2.0930624602452528
>1k	0.003693869008402822	3.804396333472778
>5k	2.8414376987714017E-4	1.0214767164692151
>10k+	4.2621565481571025E-4	4.113135058797006
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22164	1.6847680669407215	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	19681	1.4960260027729804	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12084	0.91854977986427	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8185	0.622172289654837	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5208	0.3958794483228333	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4815	0.3660060567731264	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4270	0.3245785799421079	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3838	0.29174065335311716	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3430	0.2607270560190703	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3244	0.24658850429325482	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2531	0.19239072267762886	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2127	0.161681180219406	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1815	0.13796489990513489	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1726	0.13119967891805112	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1726	0.13119967891805112	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1696	0.12891926734937123	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1662	0.12633480090486732	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1464	0.11128408455157988	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1363	0.10360669893702415	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1359	0.10330264406120016	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	7.601371895599717E-5	0.0
3	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
4	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
5	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
6	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
7	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
8	7.601371895599717E-5	0.0	0.0	7.601371895599717E-5	0.0
9	1.5202743791199435E-4	0.0	0.0	2.2804115686799153E-4	0.0
10	1.5202743791199435E-4	0.0	0.0	2.2804115686799153E-4	0.0
11	1.5202743791199435E-4	0.0	0.0	5.320960326919802E-4	0.0
12	1.5202743791199435E-4	0.0	0.0	5.320960326919802E-4	0.0
13	1.5202743791199435E-4	0.0	0.0	6.081097516479774E-4	0.0
14	2.2804115686799153E-4	0.0	0.0	6.081097516479774E-4	0.0
15	3.040548758239887E-4	0.0	0.0	7.601371895599718E-4	0.0
16	3.040548758239887E-4	0.0	0.0	9.881783464279633E-4	0.0
17	3.040548758239887E-4	0.0	0.0	0.0015202743791199436	0.0
18	3.040548758239887E-4	0.0	0.0	0.0018243292549439323	0.0
19	3.800685947799859E-4	0.0	0.0	0.0027364938824158986	0.0
20	3.800685947799859E-4	0.0	0.0	0.0052449466079638055	0.0
21	3.800685947799859E-4	0.0	0.0	0.010185838340103621	0.0
22	4.5608231373598306E-4	0.0	0.0	0.017559169078835347	0.0
23	4.5608231373598306E-4	0.0	0.0	0.022728101967843157	0.0
24	4.5608231373598306E-4	0.0	0.0	0.02911325436014692	0.0
25	4.5608231373598306E-4	0.0	0.0	0.03337002262168276	0.0
26	5.320960326919802E-4	0.0	0.0	0.04119943567415047	0.0
27	5.320960326919802E-4	0.0	0.0	0.08232285762934495	0.0
28	5.320960326919802E-4	0.0	0.0	0.161757193938362	0.0
29	5.320960326919802E-4	0.0	0.0	0.2639956459341782	0.0
30	5.320960326919802E-4	0.0	0.0	0.404849067159641	0.0
31	5.320960326919802E-4	0.0	0.0	0.5587768480455353	7.601371895599717E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5870	0.0	24.36201	1
TATACCG	40	0.0019313352	23.125	5
GCGTGTC	45	0.003826097	20.555557	11
TAGACTC	50	0.007035409	18.5	5
GTATCAA	8475	0.0	16.851917	2
GTATTGG	530	0.0	16.754717	1
CGAACTA	265	0.0	16.056604	24
TACCCGG	70	0.0025927608	15.857142	5
TCCTCGA	70	0.0025927608	15.857142	23
TAACCCG	70	0.0025927608	15.857142	5
TACACAG	235	0.0	15.744681	5
GACGTAT	145	5.3467375E-8	15.310345	28
TTAACGG	160	1.0966687E-8	15.03125	35
TTTACAC	185	3.0559022E-10	15.0	3
ACGTATA	150	8.102688E-8	14.8	29
TTTTTAC	870	0.0	14.672413	1
GGTAAGA	240	0.0	14.645833	2
GGGTAAG	205	9.276846E-11	14.439024	1
TCCGACG	90	8.275133E-4	14.388889	23
GGTTACA	90	8.275133E-4	14.388889	1
>>END_MODULE