FastQCFastQC Report
Wed 25 May 2016
SRR2088424_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088424_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1025116
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT151491.4777839776181427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133061.2979994459163646No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT79410.7746440402842215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54750.5340858985714787No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG29760.2903086089769353No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA28410.27713936764229613No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG26940.2627995270779112No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA21760.2122686603272215No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21660.2112931609691001No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT17660.17227318664424318No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG17610.17178543696518248No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14690.14330085570803694No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11230.10954857791703573No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC603.7257814E-521.5833323
CCGTATA705.101034E-621.1428592
GGTATCA45950.021.0163231
GGACTAT806.958617E-720.81256
GTAAGCG555.142573E-420.1818183
GTAAGAT1403.6379788E-1219.821433
GGGTAAG1950.018.974361
GGTAAGA1501.2732926E-1118.52
TAGTACA1201.0408439E-716.9583324
TATGCGG1851.8189894E-1116.05
TAAGGTG957.058486E-515.5789485
TGTACAC2500.015.543
GTATCAA62650.015.4437352
TAGAACT1201.9349063E-615.4166664
ATCCGTT750.00410400414.812
GTACAGC1252.957804E-614.7999996
ACTGTCC1252.957804E-614.7999998
TTATGCG1651.6438207E-814.5757574
GTAATAC1405.9924787E-714.5357153
TAAGATT2201.8189894E-1114.2954544
Produced by FastQC (version 0.11.5)