FastQCFastQC Report
Wed 25 May 2016
SRR2088423_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088423_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences679665
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT135481.993334951777714No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT110631.627713653049664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66160.9734207293298904No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49330.7257987390846962No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG27530.40505248909389185No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG24540.36106022820065764No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA22840.33604790595366835No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA22330.32854420927957156No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21020.30927000801865623No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14810.2179014661634776No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG14480.21304613302141495No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13590.19995144666857936No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC10350.15228090309196443No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC9900.145659994261879No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9880.14536573164720853No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG9380.138009166280447No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA9250.13609645928508898No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA8290.12197185378090677No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA7690.11314397534079289No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA7500.1103484805014235No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT7440.10946569265741211No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.10681732912537795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7170.10549314735936086No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT6890.10137347075397439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGTT451.3224348E-424.66666624
CAGAACG705.096863E-621.14285722
GGTATCA36450.020.606311
CTGACTG450.003824223320.5555559
ACGTTTA555.140121E-420.18181826
ATCCTAC609.2315197E-418.51
TGTATAC609.2315197E-418.53
ATAACAC500.00703199618.53
TATACTT1501.2732926E-1118.55
TAAGCCG609.2315197E-418.536
TAAGCCC609.2315197E-418.54
TAAGAGG500.00703199618.54
CGAGGCT1552.0008883E-1117.90322733
TGCACTG1156.393384E-817.6956525
TAATACT1705.456968E-1217.4117644
CTTAACC752.0657007E-417.2666663
GTACACG752.0657007E-417.2666664
ACGGGTT752.0657007E-417.2666665
GTATACT752.0657007E-417.2666664
GCCCGGT752.0657007E-417.26666622
Produced by FastQC (version 0.11.5)