##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088423_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	679665
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59018339917459	31.0	30.0	31.0	28.0	34.0
2	30.80704758962136	31.0	31.0	33.0	27.0	34.0
3	30.806058867236064	31.0	30.0	34.0	27.0	34.0
4	34.540735509405366	35.0	35.0	37.0	32.0	37.0
5	34.329378443792166	35.0	35.0	37.0	30.0	37.0
6	34.56469584280491	35.0	35.0	37.0	31.0	37.0
7	34.356793420287936	35.0	35.0	37.0	30.0	37.0
8	34.49225574363841	35.0	35.0	37.0	30.0	37.0
9	35.84247827974075	37.0	35.0	39.0	30.0	39.0
10	35.59884943317664	37.0	35.0	39.0	30.0	39.0
11	35.74790080407259	37.0	35.0	39.0	30.0	39.0
12	35.515476006562054	37.0	35.0	39.0	30.0	39.0
13	35.63489513216069	37.0	35.0	39.0	30.0	39.0
14	36.26635916223434	38.0	34.0	40.0	30.0	41.0
15	36.37890578446735	38.0	35.0	40.0	30.0	41.0
16	36.150759565374116	38.0	34.0	40.0	29.0	41.0
17	36.32300030161918	38.0	35.0	40.0	30.0	41.0
18	36.21792942111187	38.0	35.0	40.0	30.0	41.0
19	36.33897287634349	38.0	35.0	40.0	30.0	41.0
20	36.25729587370249	38.0	34.0	40.0	30.0	41.0
21	36.093741769842495	38.0	34.0	40.0	29.0	41.0
22	35.97827164853273	38.0	34.0	40.0	29.0	41.0
23	35.74268499922756	38.0	34.0	40.0	27.0	41.0
24	35.902334238190875	38.0	34.0	40.0	29.0	41.0
25	35.852773057315	38.0	34.0	40.0	28.0	41.0
26	35.56156341727174	38.0	34.0	40.0	27.0	41.0
27	35.4582007312426	38.0	34.0	40.0	27.0	41.0
28	35.21048163433456	38.0	33.0	40.0	27.0	41.0
29	35.00926927236212	38.0	33.0	40.0	26.0	41.0
30	34.77498620643994	37.0	33.0	40.0	25.0	41.0
31	34.42175630641566	37.0	33.0	40.0	25.0	41.0
32	34.23456997197149	37.0	33.0	39.0	24.0	41.0
33	33.99047324785004	36.0	33.0	39.0	24.0	40.0
34	33.80575136280373	36.0	32.0	39.0	23.0	40.0
35	33.465280689751566	36.0	31.0	39.0	22.0	40.0
36	33.30169568831704	36.0	31.0	39.0	21.0	40.0
37	33.0717059139429	36.0	31.0	39.0	20.0	40.0
38	32.90821654785814	35.0	31.0	39.0	19.0	40.0
39	32.724676127209726	35.0	31.0	39.0	18.0	40.0
40	32.52039902010549	35.0	30.0	39.0	16.0	40.0
41	32.21221042719575	35.0	30.0	39.0	15.0	40.0
42	31.874960458461153	35.0	30.0	39.0	14.0	40.0
43	31.496734420633693	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	3.0
14	6.0
15	16.0
16	31.0
17	72.0
18	199.0
19	421.0
20	859.0
21	1502.0
22	2598.0
23	4176.0
24	6003.0
25	8538.0
26	11908.0
27	15834.0
28	20201.0
29	25341.0
30	30022.0
31	35678.0
32	41084.0
33	48145.0
34	56644.0
35	66029.0
36	81925.0
37	95168.0
38	91276.0
39	35985.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.783518350952306	25.305407811201107	16.0155370660546	19.895536771791985
2	22.126341653608765	26.501438208529205	31.007481626977995	20.364738510884038
3	23.66474660310594	25.246555288267015	32.32106993886694	18.767628169760105
4	17.39371602186371	18.578417308526994	32.781296668211546	31.24657000139775
5	11.803903393583603	41.712461286074756	33.45368674273355	13.029948577608085
6	32.32283551455497	37.70254463596036	12.486298397004408	17.488321452480264
7	24.427622431639115	34.45785791529651	21.123936056733832	19.990583596330545
8	28.444012859276263	34.87747640381659	18.296219461058023	18.38229127584913
9	24.149838523390198	14.1809567948916	22.262879506815857	39.40632517490234
10	16.876255213965706	29.355638439525357	32.35226177602201	21.415844570486932
11	32.921365672794686	22.544783091670162	23.274112982130905	21.25973825340425
12	19.972633576835648	28.983249100659886	31.387963187746905	19.656154134757564
13	32.968741953756634	20.58028587613015	26.977407987758674	19.47356418235454
14	19.424128063089903	23.779361891520086	29.193646870149266	27.602863175240742
15	22.30039799018634	30.527539302450474	26.53969234843636	20.632370358926824
16	20.440805396776355	28.42885833462073	28.26112864425857	22.869207624344345
17	21.31932643287502	27.175446727431897	28.46358132315185	23.041645516541237
18	22.09191292769232	25.20535852221315	31.102234188901885	21.600494361192645
19	21.846497907057152	24.53090860938845	32.971537448596	20.651056034958398
20	22.556406464949642	24.522080730948336	32.86780987692467	20.05370292717736
21	21.745860092839855	25.359110738378465	33.55667865786822	19.338350510913465
22	20.106523066510707	25.74032795568405	32.84412173644369	21.30902724136155
23	21.236050112923277	26.059308629986834	33.73559032758786	18.969050929502036
24	21.134970904783977	25.98162329971383	31.34352953293166	21.53987626257053
25	21.03448022187401	26.57927067010954	32.053732353438825	20.33251675457762
26	20.942670286096828	27.164853273303756	32.26192315331818	19.630553287281234
27	20.0104463228208	26.991973987184863	32.0256302737378	20.97194941625654
28	20.24776912155253	27.192072565160778	32.074624999080434	20.485533314206265
29	20.523493191498755	26.191432543973868	31.591298654484195	21.693775610043183
30	20.50260054585715	26.85440621482642	33.18958604606681	19.453407193249618
31	21.12467171327051	26.77554383409474	32.745838023143754	19.353946429490996
32	20.797451685756954	26.551315721715845	31.735340204365386	20.915892388161815
33	20.526582948952793	26.96151780656647	31.81508537294108	20.696813871539653
34	19.77415344324042	27.749111694731965	32.37344868427828	20.103286177749332
35	19.169590901399953	29.753628625867158	31.55628140333841	19.520499069394482
36	21.823692554420195	26.934887039938793	31.34426518946834	19.897155216172674
37	20.57734324998345	28.11179036731331	31.00130211206992	20.309564270633327
38	20.660472438627853	26.141260768172554	32.51248776971008	20.685779023489516
39	19.97925448566573	26.178779251543038	33.32259274789786	20.519373514893367
40	18.782341300493627	27.175888121353903	32.270898163065624	21.770872415086846
41	20.570280947231357	26.121103779067628	32.20969153921417	21.098923734486842
42	19.450317435795576	28.00718000779796	31.339998381555617	21.202504174850844
43	19.755467767208845	27.8787343764943	30.514444616097634	21.851353240199213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	85.5
2	113.0
3	232.0
4	351.0
5	351.0
6	731.0
7	1111.0
8	1341.5
9	1572.0
10	2538.0
11	3504.0
12	3504.0
13	7723.5
14	11943.0
15	19621.5
16	27300.0
17	23364.5
18	19429.0
19	19429.0
20	21535.0
21	23641.0
22	18137.5
23	12634.0
24	13240.5
25	13847.0
26	13847.0
27	14638.0
28	15429.0
29	16300.5
30	17172.0
31	18495.5
32	19819.0
33	19819.0
34	21752.0
35	23685.0
36	25464.0
37	27243.0
38	28790.5
39	30338.0
40	30338.0
41	31359.5
42	32381.0
43	34219.5
44	36058.0
45	39495.0
46	42932.0
47	42932.0
48	58698.0
49	74464.0
50	68846.5
51	63229.0
52	57361.5
53	51494.0
54	51494.0
55	44824.0
56	38154.0
57	32084.5
58	26015.0
59	22726.0
60	19437.0
61	19437.0
62	16866.0
63	14295.0
64	12437.5
65	10580.0
66	9023.0
67	7466.0
68	7466.0
69	6299.5
70	5133.0
71	4268.0
72	3403.0
73	2845.0
74	2287.0
75	2287.0
76	1847.0
77	1407.0
78	1112.0
79	817.0
80	629.0
81	441.0
82	441.0
83	343.0
84	245.0
85	187.0
86	129.0
87	92.0
88	55.0
89	55.0
90	43.5
91	32.0
92	23.5
93	15.0
94	10.0
95	5.0
96	5.0
97	3.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	679665.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.25855217079988
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.30816781244836	50.86447449438474
2	7.2934006543954295	8.498059250532842
3	1.946232970761751	3.4015414519096296
4	0.8785503361635113	2.047322823762228
5	0.5135950229003944	1.4960651218152892
6	0.34924149428133755	1.2207782288878448
7	0.26905954177590163	1.0972513546122014
8	0.1873737156811757	0.873289711235872
9	0.13825826417094023	0.7249253666622258
>10	0.9760412141676278	10.976800848799769
>50	0.08359145690423612	3.3007703807397877
>100	0.047115148165829346	5.162846140109866
>500	0.006079373956869573	2.4688985543503477
>1k	0.0025330724820289887	3.258709974725904
>5k	2.5330724820289886E-4	0.9763438634537898
>10k+	5.066144964057977E-4	3.6319224340177176
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13548	1.993334951777714	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11063	1.627713653049664	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6616	0.9734207293298904	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4933	0.7257987390846962	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2753	0.40505248909389185	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	2454	0.36106022820065764	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2284	0.33604790595366835	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2233	0.32854420927957156	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2102	0.30927000801865623	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1481	0.2179014661634776	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	1448	0.21304613302141495	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1359	0.19995144666857936	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1035	0.15228090309196443	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	990	0.145659994261879	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	988	0.14536573164720853	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	938	0.138009166280447	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	925	0.13609645928508898	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	829	0.12197185378090677	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	769	0.11314397534079289	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	750	0.1103484805014235	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	744	0.10946569265741211	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.10681732912537795	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	717	0.10549314735936086	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	689	0.10137347075397439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.4713130733523132E-4	0.0
8	0.0	0.0	0.0	1.4713130733523132E-4	0.0
9	0.0	0.0	0.0	1.4713130733523132E-4	0.0
10	1.4713130733523132E-4	0.0	0.0	1.4713130733523132E-4	0.0
11	1.4713130733523132E-4	0.0	0.0	2.9426261467046264E-4	0.0
12	4.4139392200569395E-4	0.0	0.0	4.4139392200569395E-4	0.0
13	4.4139392200569395E-4	0.0	0.0	4.4139392200569395E-4	0.0
14	4.4139392200569395E-4	0.0	0.0	8.827878440113879E-4	0.0
15	4.4139392200569395E-4	0.0	0.0	0.0010299191513466193	0.0
16	4.4139392200569395E-4	0.0	0.0	0.0014713130733523133	0.0
17	4.4139392200569395E-4	0.0	0.0	0.0019127069953580073	0.0
18	4.4139392200569395E-4	0.0	0.0	0.0025012322246989326	0.0
19	4.4139392200569395E-4	0.0	0.0	0.0035311513760455516	0.0
20	4.4139392200569395E-4	0.0	0.0	0.007503696674096797	0.0
21	4.4139392200569395E-4	0.0	0.0	0.013683211582176514	0.0
22	4.4139392200569395E-4	0.0	0.0	0.023541009173637013	0.0
23	4.4139392200569395E-4	0.0	0.0	0.030603311925728117	0.0
24	4.4139392200569395E-4	0.0	0.0	0.04016684690251815	0.0
25	4.4139392200569395E-4	0.0	0.0	0.04767054357661495	0.0
26	4.4139392200569395E-4	0.0	0.0	0.05664555332406406	0.0
27	5.885252293409253E-4	0.0	0.0	0.0976951880705936	0.0
28	5.885252293409253E-4	0.0	0.0	0.1818542958663459	0.0
29	5.885252293409253E-4	0.0	0.0	0.2901429380650762	0.0
30	5.885252293409253E-4	0.0	0.0	0.44301236638638153	0.0
31	5.885252293409253E-4	0.0	0.0	0.6132432889732442	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGTT	45	1.3224348E-4	24.666666	24
CAGAACG	70	5.096863E-6	21.142857	22
GGTATCA	3645	0.0	20.60631	1
CTGACTG	45	0.0038242233	20.555555	9
ACGTTTA	55	5.140121E-4	20.181818	26
ATCCTAC	60	9.2315197E-4	18.5	1
TGTATAC	60	9.2315197E-4	18.5	3
ATAACAC	50	0.007031996	18.5	3
TATACTT	150	1.2732926E-11	18.5	5
TAAGCCG	60	9.2315197E-4	18.5	36
TAAGCCC	60	9.2315197E-4	18.5	4
TAAGAGG	50	0.007031996	18.5	4
CGAGGCT	155	2.0008883E-11	17.903227	33
TGCACTG	115	6.393384E-8	17.695652	5
TAATACT	170	5.456968E-12	17.411764	4
CTTAACC	75	2.0657007E-4	17.266666	3
GTACACG	75	2.0657007E-4	17.266666	4
ACGGGTT	75	2.0657007E-4	17.266666	5
GTATACT	75	2.0657007E-4	17.266666	4
GCCCGGT	75	2.0657007E-4	17.266666	22
>>END_MODULE